Any feedback?
Literature summary for 2.3.1.26 extracted from
- Purification of recombinant acyl-coenzyme A:cholesterol acyltransferase 1 (ACAT1) from H293 cells and binding studies between the enzyme and substrates using difference intrinsic fluorescence spectroscopy (2010), Biochemistry, 49, 9957-9963.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cholesterol | - |
Homo sapiens |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant hACAT1 (HisACAT1/Flag) is expressed in HEK293 cells | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0013 | - |
oleoyl-CoA | pH and temperature not specified in the publication | Homo sapiens | |
| 0.0064 | - |
stearoyl-CoA | pH and temperature not specified in the publication | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the enzyme activity of a stable clone of H293 cells expressing HisACAT1/Flag is about 9-10times higher than that of a stable clone of CHO cells either expressing hACAT1 or expressing HisACAT1 | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oleoyl-CoA + cholesterol | intrinsic fluorescence spectroscopy is used to study the binding between the enzyme and its substrates in CHAPS/phospholipid mixed micelles. Results show that oleoyl-CoA binds to ACAT1 with Kd=1.9 µM and elicits significant structural changes of the protein as manifested by the significantly positive changes in its fluorescence spectrum | Homo sapiens | CoA + cholesteryl oleate | - |
? | |
| stearoyl-CoA + cholesterol | - |
Homo sapiens | CoA + cholesteryl stearate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ACAT1 | - |
Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
