C333A |
expressed in H5 cells, the mutant retains a significant amount of activity, Cys-333 is not essential for ACAT1 catalysis, the mutant is expressed as a single, undegraded 50-kDA band |
Homo sapiens |
C333A/C345A/C365A |
expressed in H5 cells, the mutant contains essentially the same activity as the wild-type ACAT1. Sensitive to p-chloromercuribenzene sulfonic acid inhibition |
Homo sapiens |
C345A |
expressed in H5 cells, the mutant retains a significant amount of activity, Cys-345 is not essential for ACAT1 catalysis, the mutant is expressed as a single, undegraded 50-kDA band |
Homo sapiens |
C345A/C467A |
insensitive to p-chloromercuribenzene sulfonic acid |
Homo sapiens |
C365A |
expressed in H5 cells, the mutant retains a significant amount of activity, Cys-365 is not essential for ACAT1 catalysis, the mutant is expressed as a single, undegraded 50-kDA band |
Homo sapiens |
C387A |
expressed in H5 cells, the mutant retains a significant amount of activity, Cys-387 is not essential for ACAT1 catalysis, the mutant is expressed as a single, undegraded 50-kDA band |
Homo sapiens |
C467A |
expressed in H5 cells, the mutant retains a significant amount of activity, Cys-467 is not essential for ACAT1 catalysis, the mutant is expressed as a single, undegraded 50-kDA band |
Homo sapiens |
C516A |
expressed in H5 cells, the mutant retains a significant amount of activity, Cys-516 is not essential for ACAT1 catalysis, the mutant is expressed as a single, undegraded 50-kDA band |
Homo sapiens |
C528A |
expressed in H5 cells, the mutant retains a significant amount of activity, Cys-528 is not essential for ACAT1 catalysis, the mutant is expressed as a single, undegraded 50-kDA band |
Homo sapiens |
C546A |
expressed in H5 cells, the mutant retains a significant amount of activity , Cys-546 is not essential for ACAT1 catalysis, the mutant is expressed as a single, undegraded 50-kDA band |
Homo sapiens |
C92A |
expressed in H5 cells, the mutant retains a significant amount of activity, Cys-92 is not essential for ACAT1 catalysis, the mutant is expressed as a single, undegraded 50-kDA band |
Homo sapiens |
C92A/C333A/C345A/C365A/C387A/C467A/C516A |
resistant to p-chloromercuribenzene sulfonic acid inhibition |
Homo sapiens |
C92A/C333A/C345A/C365A/C387A/C467A/C516A/C528A/C54 |
expressed in H5 cells or in ACAT-deficient Chinese hamster ovary cells: both mutants retain partial enzyme activity |
Homo sapiens |
C92A/C333A/C345A/C365A/C387A/C467A/C516A/C528A/C54 |
this mutant is localized in endoplasmic reticulum in Chinese hamster ovary cells, homotetramer. Insensitive to p-chloromercuribenzene sulfonic acid |
Homo sapiens |
C92A/C333A/C345A/C365A/C387A/C467A/C516A/C546A |
this mutant is localized in endoplasmic reticulum in Chinese hamster ovary cells |
Homo sapiens |
C92A/C333A/C345A/C365A/C387A/C467A/C516A/C546A |
expressed in H5 cells or in ACAT-deficient Chinese hamster ovary cells: both mutants retain partial enzyme activity. Resistant to p-chloromercuribenzene sulfonic acid inhibition |
Homo sapiens |
C92A/C333A/C345A/C365A/C387A/C516A/C528A/C546A |
sensitive to p-chloromercuribenzene sulfonic acid inhibition |
Homo sapiens |
C92A/C333A/C345A/C365A/C467A/C516A/C546A |
expressed in H5 cells or in ACAT-deficient Chinese hamster ovary cells: both mutants retain partial enzyme activity. Resistant to p-chloromercuribenzene sulfonic acid inhibition |
Homo sapiens |
C92A/C333A/C365A/C387A/C467A/C516A/C528A/C546A |
insensitive to p-chloromercuribenzene sulfonic acid |
Homo sapiens |
C92A/C467A/C516A/C546A |
expressed in H5 cells or in ACAT-deficient Chinese hamster ovary cells: both mutants retain partial enzyme activity |
Homo sapiens |
C92A/C467A/C516A/C546A |
this mutant is localized in endoplasmic reticulum in Chinese hamster ovary cells |
Homo sapiens |