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Literature summary for 2.3.1.26 extracted from

  • Becker, A.; Böttcher, A.; Lackner, K.J.; Fehringer, P.; Notka, F.; Aslanidis, C.; Schmitz, G.
    Purification, cloning, and expression of a human enzyme with acyl coenzyme A:cholesterol acyltransferase activity, which is identical to liver carboxylesterase (1994), Arterioscler. Thromb., 14, 1346-1355.
    View publication on PubMed
Search for more literature for 2.3.1.26

Cloned(Commentary)

Cloned (Comment) Organism
expression in enzyme-deficient mutant chinese hamster ovary cells Homo sapiens
expression of an antisense cDNA in HepG2 cells Homo sapiens
sequence analysis and human cDNA clones indicate homology to liver carboxylesterase Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Homo sapiens
-
-
microsome
-
 Sus scrofa
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 x * 58000, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
long-chain fatty acyl-CoA + cholesterol Sus scrofa
-
 CoA + cholesteryl long-chain fatty acyl ester
-
 ?
long-chain fatty acyl-CoA + cholesterol Homo sapiens the enzyme is relevant for cellular cholesterol esterification in vivo, the regulation in human mononuclear phagocytes indicates that the enzyme is also involved in foam cell formation during early atherogenesis CoA + cholesteryl long-chain fatty acyl ester
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
based on solubilization in CHAPS, preparative free-flow isotachophoresis, and further separation of the protein showing enzyme activity by preparative free-flow IEF, followed by removal of nonglycosylated proteins by ConA-affinity chromatography and preparative SDS-PAGE Sus scrofa

Renatured (Commentary)

Renatured (Comment) Organism
reconstitution with liposomes Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-
liver
-
 Sus scrofa
-
macrophage
-
 Homo sapiens
-
monocyte
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
long-chain fatty acyl-CoA + cholesterol
-
 Sus scrofa CoA + cholesteryl long-chain fatty acyl ester
-
 ?
long-chain fatty acyl-CoA + cholesterol the enzyme is relevant for cellular cholesterol esterification in vivo, the regulation in human mononuclear phagocytes indicates that the enzyme is also involved in foam cell formation during early atherogenesis Homo sapiens CoA + cholesteryl long-chain fatty acyl ester
-
 ?
oleoyl-CoA + cholesterol
-
 Homo sapiens CoA + cholesteryl oleate
-
 ?
oleoyl-CoA + cholesterol
-
 Sus scrofa CoA + cholesteryl oleate
-
 ?

Subunits

Subunits Comment Organism
? x * 58000, SDS-PAGE Sus scrofa