Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidylethanolamine | weak stimulatory effect on activity | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphatidylinositol | - |
Sus scrofa | |
phosphatidylserine | - |
Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Sus scrofa | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial, using ammonium acetate fractionation and Sepharose 4B column chromatography | Sus scrofa |
Renatured (Comment) | Organism |
---|---|
solubilization of enzyme activity by deoxycholate, reconstitution of the solubilized activity into liposome of known cholesterol and phospholipid content by employing a cholate dialysis procedure | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00008 | - |
solubilized enzyme | Sus scrofa |
0.015 | - |
partially purified enzyme | Sus scrofa |
Storage Stability | Organism |
---|---|
4°C, Tris buffer, 1 mM EDTA, pH 7.7, 6.0 mg/ml protein, 5.0 mg/ml cholate, 5.5 mg/ml deoxycholate, 0.5 M potassium chloride, solubilized enzyme, stable for at least 18 h | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + cholesterol | - |
Sus scrofa | CoA + cholesterol ester | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Sus scrofa |