Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star(DE3) cells | Homo sapiens |
recombinant expression of hNaa40p in Escherichia coli strain BL21 StarTM (DE3), and of hNaa40p-V5 in HEK293 cells. Heterologous ectopically expression of hNaa40p in a Saccharomyces cerevisiae naa40-DELTA strain restores Nt-acetylation of yeast histone H4, but not H2A in vivo | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
additional information | endogenous hNaa40p localizes to both the cytoplasm and the nuclei | Homo sapiens | - |
- |
nucleus | - |
Homo sapiens | 5634 | - |
ribosome | - |
Homo sapiens | 5840 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + an N-terminal-L-seryl-[histone H2A] | Homo sapiens | - |
an N-terminal-Nalpha-acetyl-L-seryl-[histone H2A] + CoA | - |
? | |
acetyl-CoA + an N-terminal-L-seryl-[histone H4] | Homo sapiens | - |
an N-terminal-Nalpha-acetyl-L-seryl-[histone H4] + CoA | - |
? | |
acetyl-CoA + peptide | Homo sapiens | hNaa40p preferentially acetylates Ser-Gly-Gly-Gly-Lys- starting peptides | CoA + Nalpha-acetylpeptide | - |
? | |
additional information | Homo sapiens | hNaa40p is a NAT selectively acetylating Ser-Gly or Gly-Gly starting peptides. NatD also is defined as the catalytic unit Naa40p (Nat4) which co-translationally Nt-acetylates histones H2A and H4. Recombinant hNaa40p Nt-acetylates the oligopeptides derived from the N-termini of both histones | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q86UY6 | - |
- |
Homo sapiens | Q86UY6 | NatD | - |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Superdex 75 gel filtration | Homo sapiens |
recombinant hNaa40p-V5 from HEK293 cells by immunoprecipitation | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-431 cell | - |
Homo sapiens | - |
HEK-293 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + an N-terminal-L-seryl-[histone H2A] | - |
Homo sapiens | an N-terminal-Nalpha-acetyl-L-seryl-[histone H2A] + CoA | - |
? | |
acetyl-CoA + an N-terminal-L-seryl-[histone H4] | - |
Homo sapiens | an N-terminal-Nalpha-acetyl-L-seryl-[histone H4] + CoA | - |
? | |
acetyl-CoA + histone H2A | Saccharomyces cerevisiae histone H2A, 2SGGKGGKAGSAAKASQSR19 N-terminal sequence | Homo sapiens | CoA + Nalpha-acetyl-histone H2A | - |
? | |
acetyl-CoA + histone H4 | Saccharomyces cerevisiae histone H4, 2SGRGKGGKGLGKGGAKR18 N-terminal sequence. hNaa40p catalyzes H4 Nalpha-acetylation and not H4 lysine Nepsilon-acetylation, immunoprecipitated hNaa40p specifically Nt-acetylates H4 in vitro | Homo sapiens | CoA + Nalpha-acetyl-histone H4 | - |
? | |
acetyl-CoA + peptide | - |
Homo sapiens | CoA + Nalpha-acetylpeptide | - |
? | |
acetyl-CoA + peptide | hNaa40p preferentially acetylates Ser-Gly-Gly-Gly-Lys- starting peptides | Homo sapiens | CoA + Nalpha-acetylpeptide | - |
? | |
additional information | hNaa40p is a NAT selectively acetylating Ser-Gly or Gly-Gly starting peptides. NatD also is defined as the catalytic unit Naa40p (Nat4) which co-translationally Nt-acetylates histones H2A and H4. Recombinant hNaa40p Nt-acetylates the oligopeptides derived from the N-termini of both histones | Homo sapiens | ? | - |
? | |
additional information | specificity determination using an in vitro proteome-derived peptide library Nt-acetylation assay, in vitro acetylation using synthetic oligopeptides as substrates, overview | Homo sapiens | ? | - |
? | |
additional information | the enzyme does not catalyze H4 lysine Nepsilon-acetylation | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
hNaa40p/hNatD | - |
Homo sapiens |
N-alpha-acetyltransferase 40 | - |
Homo sapiens |
Naa40p | - |
Homo sapiens |
Naa40p | catalytic subunit of NatD, also termed Nat4 | Homo sapiens |
NAT4 | - |
Homo sapiens |
NatD | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Homo sapiens |