Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Thermoplasma acidophilum |
expressed in Escherichia coli Rosetta II(DE3)pLysS cells | Thermoplasma acidophilum |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.1 M sodium acetate pH 4.6, 8% (w/v) polyethylene glycol 4000 and 35% (v/v) glycerol | Thermoplasma acidophilum |
hanging drop vapor diffusion method, using 0.1 M trisodium citrate pH 5.6, 20% (v/v) 2-propanol, 20% (w/v) polyethylene glycol 4000 and 0.2 M sodium chloride | Thermoplasma acidophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoplasma acidophilum | Q9HJ33 | - |
- |
Thermoplasma acidophilum | Q9HM13 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography and Superdex 200 gel filtration | Thermoplasma acidophilum |
Synonyms | Comment | Organism |
---|---|---|
Ta0058 | - |
Thermoplasma acidophilum |
Ta1140 | - |
Thermoplasma acidophilum |