Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of Naa25-/- MEF cells and of NAA25-knockout mice | Mus musculus |
additional information | generation yeast naa20DELTA cells, W303 naa20DELTA, from wild-type strain W303-1B | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Saccharomyces cerevisiae | 5737 | - |
cytoplasm | - |
Mus musculus | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + an N-terminal L-methionyl-L-asparaginyl-[protein] | Saccharomyces cerevisiae | - |
an N-terminal Nalpha-acetyl-L-methionyl-L-asparginyl-[protein] + CoA | - |
? | |
acetyl-CoA + an N-terminal L-methionyl-L-asparaginyl-[protein] | Mus musculus | - |
an N-terminal Nalpha-acetyl-L-methionyl-L-asparginyl-[protein] + CoA | - |
? | |
acetyl-CoA + an N-terminal L-methionyl-L-asparaginyl-[protein] | Mus musculus C57BL/6 | - |
an N-terminal Nalpha-acetyl-L-methionyl-L-asparginyl-[protein] + CoA | - |
? | |
acetyl-CoA + an N-terminal L-methionyl-L-asparaginyl-[protein] | Saccharomyces cerevisiae ATCC 204508 | - |
an N-terminal Nalpha-acetyl-L-methionyl-L-asparginyl-[protein] + CoA | - |
? | |
acetyl-CoA + an N-terminal L-methionyl-L-aspartyl-[protein] | Saccharomyces cerevisiae | - |
an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[protein] + CoA | - |
? | |
acetyl-CoA + an N-terminal L-methionyl-L-aspartyl-[protein] | Mus musculus | - |
an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[protein] + CoA | - |
? | |
acetyl-CoA + an N-terminal L-methionyl-L-aspartyl-[protein] | Mus musculus C57BL/6 | - |
an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[protein] + CoA | - |
? | |
acetyl-CoA + an N-terminal L-methionyl-L-aspartyl-[protein] | Saccharomyces cerevisiae ATCC 204508 | - |
an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[protein] + CoA | - |
? | |
acetyl-CoA + an N-terminal L-methionyl-L-glutaminyl-[protein] | Saccharomyces cerevisiae | - |
an N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[protein] + CoA | - |
? | |
acetyl-CoA + an N-terminal L-methionyl-L-glutaminyl-[protein] | Mus musculus | - |
an N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[protein] + CoA | - |
? | |
acetyl-CoA + an N-terminal L-methionyl-L-glutaminyl-[protein] | Mus musculus C57BL/6 | - |
an N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[protein] + CoA | - |
? | |
acetyl-CoA + an N-terminal L-methionyl-L-glutaminyl-[protein] | Saccharomyces cerevisiae ATCC 204508 | - |
an N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[protein] + CoA | - |
? | |
acetyl-CoA + an N-terminal L-methionyl-L-glutamyl-[protein] | Saccharomyces cerevisiae | - |
an N-terminal Nalpha-acetyl-L-methionyl-L-glutamyl-[protein] + CoA | - |
? | |
acetyl-CoA + an N-terminal L-methionyl-L-glutamyl-[protein] | Mus musculus | - |
an N-terminal Nalpha-acetyl-L-methionyl-L-glutamyl-[protein] + CoA | - |
? | |
acetyl-CoA + an N-terminal L-methionyl-L-glutamyl-[protein] | Mus musculus C57BL/6 | - |
an N-terminal Nalpha-acetyl-L-methionyl-L-glutamyl-[protein] + CoA | - |
? | |
acetyl-CoA + an N-terminal L-methionyl-L-glutamyl-[protein] | Saccharomyces cerevisiae ATCC 204508 | - |
an N-terminal Nalpha-acetyl-L-methionyl-L-glutamyl-[protein] + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P61600 AND Q8BWZ3 | subunits Naa20 and Naa25 of enzyme complex NatB | - |
Mus musculus C57BL/6 | P61600 AND Q8BWZ3 | subunits Naa20 and Naa25 of enzyme complex NatB | - |
Saccharomyces cerevisiae | Q06504 AND Q12387 | subunits Nat3p and Mdm20p of enzyme complex NatB | - |
Saccharomyces cerevisiae ATCC 204508 | Q06504 AND Q12387 | subunits Nat3p and Mdm20p of enzyme complex NatB | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
MEF cell | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + an N-terminal L-methionyl-L-asparaginyl-[protein] | - |
Saccharomyces cerevisiae | an N-terminal Nalpha-acetyl-L-methionyl-L-asparginyl-[protein] + CoA | - |
? | |
acetyl-CoA + an N-terminal L-methionyl-L-asparaginyl-[protein] | - |
Mus musculus | an N-terminal Nalpha-acetyl-L-methionyl-L-asparginyl-[protein] + CoA | - |
? | |
acetyl-CoA + an N-terminal L-methionyl-L-asparaginyl-[protein] | - |
Mus musculus C57BL/6 | an N-terminal Nalpha-acetyl-L-methionyl-L-asparginyl-[protein] + CoA | - |
? | |
acetyl-CoA + an N-terminal L-methionyl-L-asparaginyl-[protein] | - |
Saccharomyces cerevisiae ATCC 204508 | an N-terminal Nalpha-acetyl-L-methionyl-L-asparginyl-[protein] + CoA | - |
? | |
acetyl-CoA + an N-terminal L-methionyl-L-aspartyl-[protein] | - |
Saccharomyces cerevisiae | an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[protein] + CoA | - |
? | |
acetyl-CoA + an N-terminal L-methionyl-L-aspartyl-[protein] | - |
Mus musculus | an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[protein] + CoA | - |
? | |
acetyl-CoA + an N-terminal L-methionyl-L-aspartyl-[protein] | - |
Mus musculus C57BL/6 | an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[protein] + CoA | - |
? | |
acetyl-CoA + an N-terminal L-methionyl-L-aspartyl-[protein] | - |
Saccharomyces cerevisiae ATCC 204508 | an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[protein] + CoA | - |
? | |
acetyl-CoA + an N-terminal L-methionyl-L-glutaminyl-[protein] | - |
Saccharomyces cerevisiae | an N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[protein] + CoA | - |
? | |
acetyl-CoA + an N-terminal L-methionyl-L-glutaminyl-[protein] | - |
Mus musculus | an N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[protein] + CoA | - |
? | |
acetyl-CoA + an N-terminal L-methionyl-L-glutaminyl-[protein] | - |
Mus musculus C57BL/6 | an N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[protein] + CoA | - |
? | |
acetyl-CoA + an N-terminal L-methionyl-L-glutaminyl-[protein] | - |
Saccharomyces cerevisiae ATCC 204508 | an N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[protein] + CoA | - |
? | |
acetyl-CoA + an N-terminal L-methionyl-L-glutamyl-[protein] | - |
Saccharomyces cerevisiae | an N-terminal Nalpha-acetyl-L-methionyl-L-glutamyl-[protein] + CoA | - |
? | |
acetyl-CoA + an N-terminal L-methionyl-L-glutamyl-[protein] | - |
Mus musculus | an N-terminal Nalpha-acetyl-L-methionyl-L-glutamyl-[protein] + CoA | - |
? | |
acetyl-CoA + an N-terminal L-methionyl-L-glutamyl-[protein] | - |
Mus musculus C57BL/6 | an N-terminal Nalpha-acetyl-L-methionyl-L-glutamyl-[protein] + CoA | - |
? | |
acetyl-CoA + an N-terminal L-methionyl-L-glutamyl-[protein] | - |
Saccharomyces cerevisiae ATCC 204508 | an N-terminal Nalpha-acetyl-L-methionyl-L-glutamyl-[protein] + CoA | - |
? | |
acetyl-CoA + N-terminal L-methionyl-L-aspartyl-[Bax] | heterologous expression of human Bax in mouse embryonic fibroblasts (MEFs). Recombinant human Bax is N-terminally (Nt-)acetylated by Naa20 and that Nt-acetylation of Bax is essential to maintain Bax in an inactive conformation in the cytosol of MEF cells. Human Bax displays the N-terminal Met-Asp amino-acid sequence | Mus musculus | an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[Bax] + CoA | - |
? | |
acetyl-CoA + N-terminal L-methionyl-L-aspartyl-[Bax] | heterologous expression of human Bax in Saccharomyces cerevisiae. Recombinant human Bax is N-terminal (Nt-)acetylated by yNaa20p and that Nt-acetylation of Bax is essential to maintain Bax in an inactive conformation in the cytosol of yeast. Human Bax displays the N-terminal Met-Asp amino-acid sequence | Saccharomyces cerevisiae | an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[Bax] + CoA | - |
? | |
acetyl-CoA + N-terminal L-methionyl-L-aspartyl-[Bax] | heterologous expression of human Bax in mouse embryonic fibroblasts (MEFs). Recombinant human Bax is N-terminally (Nt-)acetylated by Naa20 and that Nt-acetylation of Bax is essential to maintain Bax in an inactive conformation in the cytosol of MEF cells. Human Bax displays the N-terminal Met-Asp amino-acid sequence | Mus musculus C57BL/6 | an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[Bax] + CoA | - |
? | |
acetyl-CoA + N-terminal L-methionyl-L-aspartyl-[Bax] | heterologous expression of human Bax in Saccharomyces cerevisiae. Recombinant human Bax is N-terminal (Nt-)acetylated by yNaa20p and that Nt-acetylation of Bax is essential to maintain Bax in an inactive conformation in the cytosol of yeast. Human Bax displays the N-terminal Met-Asp amino-acid sequence | Saccharomyces cerevisiae ATCC 204508 | an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[Bax] + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | the NatB enzymatic complex is constituted of two subunits, the NAA20 catalytic subunit and the accessory subunit NAA25 | Mus musculus |
heterodimer | the NatB enzymatic complex is constituted of two subunits, the Naa3p catalytic subunit and the accessory subunit MDM20 | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
MDM20 | - |
Saccharomyces cerevisiae |
Mdm20p | - |
Saccharomyces cerevisiae |
N-terminal acetyltransferase B complex catalytic subunit NAA20 | UniProt | Mus musculus |
NAA20 | - |
Mus musculus |
NAA25 | - |
Mus musculus |
NAT3 | - |
Saccharomyces cerevisiae |
Nat3p | - |
Saccharomyces cerevisiae |
natB | - |
Saccharomyces cerevisiae |
natB | - |
Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Saccharomyces cerevisiae | |
acetyl-CoA | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | Naa25 inactivation is embryonic lethal in homozygocity. Recombinant Bax accumulates in the mitochondria of Naa25-/- MEF cells, but does not promote cytochrome c release, suggesting that an additional step is required for full activation of Bax | Mus musculus |
malfunction | recombinant Bax accumulates in the mitochondria of yeast naa20DELTA (W303 naa20DELTA), but does not promote cytochrome c release, suggesting that an additional step is required for full activation of Bax | Saccharomyces cerevisiae |
additional information | the NatB enzymatic complex is constituted of two subunits, the NAA20 catalytic subunit and the accessory subunit NAA25 | Mus musculus |
additional information | the NatB enzymatic complex is constituted of two subunits, the Naa3p catalytic subunit and the accessory subunit MDM20 | Saccharomyces cerevisiae |
physiological function | recombinant human Bax expressed in MEF cells is N-terminal (Nt-)acetylated by Naa20p. Nt-acetylation of Bax is essential to maintain Bax in an inactive conformation in the cytosol of the cells. N-terminal acetylation modulates Bax targeting to mitochondria. Contribution of Bax Nt-acetylation to its regulation | Mus musculus |
physiological function | recombinant human Bax expressed in strain BY4743 is N-terminally (Nt-)acetylated by yNaa20p. Nt-acetylation of Bax is essential to maintain Bax in an inactive conformation in the cytosol of yeast. N-terminal acetylation modulates Bax targeting to mitochondria. Contribution of Bax Nt-acetylation to its regulation | Saccharomyces cerevisiae |