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Literature summary for 2.3.1.253 extracted from
- Exploiting genetic diversity by directed evolution: molecular breeding of type III polyketide synthases improves productivity (2008), Mol. Biosyst., 4, 246-248.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Pseudomonas fluorescens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A289R | the mutant shows 1.4fold improved catalytic efficiency compared to the wild type enzyme | Pseudomonas fluorescens |
| K210L | the mutant shows 1.9fold improved catalytic efficiency compared to the wild type enzyme | Pseudomonas fluorescens |
| K210L/Y256R | the mutant shows 0.9fold improved catalytic efficiency compared to the wild type enzyme | Pseudomonas fluorescens |
| Y256R | the mutant shows 1.9fold improved catalytic efficiency compared to the wild type enzyme | Pseudomonas fluorescens |
| Y256R/A289R | the mutant shows 0.8fold improved catalytic efficiency compared to the wild type enzyme | Pseudomonas fluorescens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0101 | - |
malonyl-CoA | mutant enzyme A289R, pH and temperature not specified in the publication | Pseudomonas fluorescens |  |
| 0.0103 | - |
malonyl-CoA | mutant enzyme Y256R/A289R, pH and temperature not specified in the publication | Pseudomonas fluorescens | |
| 0.0112 | - |
malonyl-CoA | mutant enzyme Y256R, pH and temperature not specified in the publication | Pseudomonas fluorescens | |
| 0.0119 | - |
malonyl-CoA | mutant enzyme K210L, pH and temperature not specified in the publication | Pseudomonas fluorescens | |
| 0.0131 | - |
malonyl-CoA | wild type enzyme, pH and temperature not specified in the publication | Pseudomonas fluorescens | |
| 0.0135 | - |
malonyl-CoA | mutant enzyme K210L/Y256R, pH and temperature not specified in the publication | Pseudomonas fluorescens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 77000 | - |
- |
Pseudomonas fluorescens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3 malonyl-CoA | Pseudomonas fluorescens | - |
phloroglucinol + 3 CO2 + 3 CoA | - |
? | |
| 3 malonyl-CoA | Pseudomonas fluorescens Pf-5 | - |
phloroglucinol + 3 CO2 + 3 CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | Q4K418 | - |
- |
| Pseudomonas fluorescens Pf-5 | Q4K418 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3 malonyl-CoA | - |
Pseudomonas fluorescens | phloroglucinol + 3 CO2 + 3 CoA | - |
? | |
| 3 malonyl-CoA | - |
Pseudomonas fluorescens Pf-5 | phloroglucinol + 3 CO2 + 3 CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | - |
Pseudomonas fluorescens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PhlD | - |
Pseudomonas fluorescens |
| phloroglucinol synthase | - |
Pseudomonas fluorescens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
the enzyme has a half-life of 7.2 min at 37°C | Pseudomonas fluorescens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.08 | - |
malonyl-CoA | mutant enzyme Y256R/A289R, pH and temperature not specified in the publication | Pseudomonas fluorescens | |
| 0.11 | - |
malonyl-CoA | mutant enzyme K210L/Y256R, pH and temperature not specified in the publication | Pseudomonas fluorescens | |
| 0.12 | - |
malonyl-CoA | wild type enzyme, pH and temperature not specified in the publication | Pseudomonas fluorescens | |
| 0.13 | - |
malonyl-CoA | mutant enzyme A289R, pH and temperature not specified in the publication | Pseudomonas fluorescens | |
| 0.2 | - |
malonyl-CoA | mutant enzyme Y256R, pH and temperature not specified in the publication | Pseudomonas fluorescens | |
| 0.21 | - |
malonyl-CoA | mutant enzyme K210L, pH and temperature not specified in the publication | Pseudomonas fluorescens | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.5 | - |
malonyl-CoA | mutant enzyme Y256R/A289R, pH and temperature not specified in the publication | Pseudomonas fluorescens | |
| 8.3 | - |
malonyl-CoA | mutant enzyme K210L/Y256R, pH and temperature not specified in the publication | Pseudomonas fluorescens | |
| 9.3 | - |
malonyl-CoA | wild type enzyme, pH and temperature not specified in the publication | Pseudomonas fluorescens | |
| 12.8 | - |
malonyl-CoA | mutant enzyme A289R, pH and temperature not specified in the publication | Pseudomonas fluorescens | |
| 17.5 | - |
malonyl-CoA | mutant enzyme K210L, pH and temperature not specified in the publication | Pseudomonas fluorescens | |
| 17.7 | - |
malonyl-CoA | mutant enzyme Y256R, pH and temperature not specified in the publication | Pseudomonas fluorescens | |
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