Cloned (Comment) | Organism |
---|---|
gene At2g25150, quantitative real-time RT-PCR expression analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 Rosetta (DE3) pLysS | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0106 | - |
4-coumaroyl-CoA | pH 7.3, 30°C, recombinant His6-tagged enzyme | Arabidopsis thaliana | |
0.0527 | - |
spermidine | pH 7.3, 30°C, recombinant His6-tagged enzyme | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 4-coumaroyl-CoA + spermidine | Arabidopsis thaliana | the enzyme shows spermidine coumaroyl CoA acyltransferase activity both in vitro and in vivo | 2 CoA + N1,N8-bis(4-coumaroyl)-spermidine | - |
? | |
2 4-coumaroyl-CoA + spermidine | Arabidopsis thaliana Col-0 | the enzyme shows spermidine coumaroyl CoA acyltransferase activity both in vitro and in vivo | 2 CoA + N1,N8-bis(4-coumaroyl)-spermidine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q8GYW8 | gene At2g25150 | - |
Arabidopsis thaliana Col-0 | Q8GYW8 | gene At2g25150 | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21 Rosetta (DE3) pLysS by nickel affinity chromatography and desalting gel filtration | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | the enzyme is expressed specifically in roots | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 4-coumaroyl-CoA + spermidine | the enzyme shows spermidine coumaroyl CoA acyltransferase activity both in vitro and in vivo | Arabidopsis thaliana | 2 CoA + N1,N8-bis(4-coumaroyl)-spermidine | - |
? | |
2 4-coumaroyl-CoA + spermidine | the enzyme shows spermidine coumaroyl CoA acyltransferase activity both in vitro and in vivo and is absolutely specific for 4-coumaroyl-CoA and spermidine | Arabidopsis thaliana | 2 CoA + N1,N8-bis(4-coumaroyl)-spermidine | - |
? | |
2 4-coumaroyl-CoA + spermidine | the enzyme shows spermidine coumaroyl CoA acyltransferase activity both in vitro and in vivo | Arabidopsis thaliana Col-0 | 2 CoA + N1,N8-bis(4-coumaroyl)-spermidine | - |
? | |
2 4-coumaroyl-CoA + spermidine | the enzyme shows spermidine coumaroyl CoA acyltransferase activity both in vitro and in vivo and is absolutely specific for 4-coumaroyl-CoA and spermidine | Arabidopsis thaliana Col-0 | 2 CoA + N1,N8-bis(4-coumaroyl)-spermidine | - |
? | |
additional information | no activity with acyl donors sinapoyl-CoA, caffeoyl-CoA, and feruloyl-CoA, and also no activity with acyl acceptors putrescine, cadaverine, spermine, agmatine, and tyramine | Arabidopsis thaliana | ? | - |
? | |
additional information | no activity with acyl donors sinapoyl-CoA, caffeoyl-CoA, and feruloyl-CoA, and also no activity with acyl acceptors putrescine, cadaverine, spermine, agmatine, and tyramine | Arabidopsis thaliana Col-0 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BAHD acyl transferase | - |
Arabidopsis thaliana |
SCT | - |
Arabidopsis thaliana |
spermidine coumaroyl CoA acyltransferase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.1 | - |
spermidine | pH 7.3, 30°C, recombinant His6-tagged enzyme | Arabidopsis thaliana | |
3.4 | - |
4-coumaroyl-CoA | pH 7.3, 30°C, recombinant His6-tagged enzyme | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
assay at | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the BAHD acyltransferase family | Arabidopsis thaliana |