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Literature summary for 2.3.1.246 extracted from
- Role of the active site cysteine of DpgA, a bacterial type III polyketide synthase (2004), Biochemistry, 43, 970-980.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C160A | kcat/Km for malonyl-CoA is 0.16% compared to the kcat/Km-value of the wild-type enzyme. The mutant shows an increased partition ratio for malonyl-CoA decarboxylation to acetyl-CoA vs condensation to 3,5-dihydroxyphenylacetyl-CoA, reflecting more uncoupling in the mutant enzyme | Amycolatopsis mediterranei |
| C160S | kcat/Km for malonyl-CoA is 2.3% compared to the kcat/Km-value of the wild-type enzyme | Amycolatopsis mediterranei |
| C190A | mutant retains wild-type activity | Amycolatopsis mediterranei |
| H296A | mutant has a 6fold drop in kcat | Amycolatopsis mediterranei |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
malonyl-CoA | pH 7.5, 24°C, wild-type enzyme | Amycolatopsis mediterranei |  |
| 0.13 | - |
malonyl-CoA | pH 7.5, 24°C, mutant enzyme C160S | Amycolatopsis mediterranei | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4 malonyl-CoA | Amycolatopsis mediterranei | the enzyme is involved in biosynthesis of 3,5-dihydroxyphenylglycine, a key nonproteinogenic residue in the vancomycin family of antibiotics | 3,5-dihydroxyphenylacetyl-CoA + 3 CoA + 4 CO2 + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Amycolatopsis mediterranei | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Amycolatopsis mediterranei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4 malonyl-CoA | - |
Amycolatopsis mediterranei | 3,5-dihydroxyphenylacetyl-CoA + 3 CoA + 4 CO2 + H2O | - |
? | |
| 4 malonyl-CoA | the enzyme is involved in biosynthesis of 3,5-dihydroxyphenylglycine, a key nonproteinogenic residue in the vancomycin family of antibiotics | Amycolatopsis mediterranei | 3,5-dihydroxyphenylacetyl-CoA + 3 CoA + 4 CO2 + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dpgA | - |
Amycolatopsis mediterranei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 24 | - |
assay at | Amycolatopsis mediterranei |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0029 | - |
malonyl-CoA | pH 7.5, 24°C, mutant enzyme C160S | Amycolatopsis mediterranei | |
| 0.0135 | - |
malonyl-CoA | pH 7.5, 24°C, wild-type enzyme | Amycolatopsis mediterranei | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Amycolatopsis mediterranei |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
malonyl-CoA | pH 7.5, 24°C, mutant enzyme C160S | Amycolatopsis mediterranei | |
| 0.9 | - |
malonyl-CoA | pH 7.5, 24°C, wild-type enzyme | Amycolatopsis mediterranei | |
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Release 2023.1 (January 2023)
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