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Literature summary for 2.3.1.24 extracted from

  • White-Gilbertson, S.; Mullen, T.; Senkal, C.; Lu, P.; Ogretmen, B.; Obeid, L.; Voelkel-Johnson, C.
    Ceramide synthase 6 modulates TRAIL sensitivity and nuclear translocation of active caspase-3 in colon cancer cells (2009), Oncogene, 28, 1132-1141 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.24

Cloned(Commentary)

Cloned (Comment) Organism
gene CERS6, recombinant expression of N-terminally FLAG-tagged CerS6 and EGFP-tagged CerS6 in SW-480 cells and localization in the perinuclear region. When cells transfected with pCerS6-IRES2-EGFP are treated with TRAIL, GFP positive cells appear to be undergoing apoptosis. Cells stably transfected with CerS6 but not with the empty plasmid acquire a TRAIL sensitive phenotype Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum membrane
-
 Homo sapiens 5789
-
perinuclear space recombinant FLAG-tagged CerS6 localizes primarily to the perinuclear region Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sphinganine + palmitoyl-CoA Homo sapiens
-
 N-palmitoylsphinganine + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q6ZMG9
-
-

Source Tissue

Source Tissue Comment Organism Textmining
colonic cancer cell
-
 Homo sapiens
-
intestine high expression level of CerS6 Homo sapiens
-
SW-480 cell
-
 Homo sapiens
-
SW-620 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sphinganine + palmitoyl-CoA
-
 Homo sapiens N-palmitoylsphinganine + CoA
-
 ?

Synonyms

Synonyms Comment Organism
ceramide synthase 6
-
 Homo sapiens
CerS6
-
 Homo sapiens
Lass6
-
 Homo sapiens
longevity assurance homolog 6
-
 Homo sapiens

General Information

General Information Comment Organism
malfunction RNAi against CerS6 results in a specific decrease in intracellular C16-ceramide and protects SW-480 cells against TRAIL-mediated apoptosis while increasing CerS6 expression sensitizes SW-620 cells to TRAIL. Downregulation of CerS6 does not interfere with caspase activation but appears to inhibit translocation of activated caspase-3 into the nucleus Homo sapiens
additional information recombinant FLAG-tagged CerS6 localizes primarily to the perinuclear region Homo sapiens
physiological function ceramide synthase 6 modulates TRAIL sensitivity and nuclear translocation of active caspase-3 in colon cancer cells. Ceramide synthase 6 (CerS6 also known as longevity assurance homolog 6/LASS6) preferentially generates C16-ceramide and can influence TRAIL susceptibility. CerS6 may regulate events at the nuclear membrane and allow late stage apoptotic signaling Homo sapiens