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Literature summary for 2.3.1.24 extracted from

  • Lim, X.Y.; Pickford, R.; Don, A.S.
    Assaying ceramide synthase activity in vitro and in living cells using liquid chromatography-mass spectrometry (2016), Methods Mol. Biol., 1376, 11-22 .
    View publication on PubMed
Search for more literature for 2.3.1.24

Inhibitors

Inhibitors Comment Organism Structure
fumonisin B1
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cell membrane
-
 Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dihydrosphingosine + myristoyl-CoA Homo sapiens
-
 N-myristoyldihydrosphingosine + CoA
-
 ?
dihydrosphingosine + palmitoyl-CoA Homo sapiens
-
 N-palmitoyldihydrosphingosine + CoA
-
 ?
dihydrosphingosine + palmitoyl-CoA Homo sapiens best substrate N-palmitoyldihydrosphingosine + CoA
-
 ?
dihydrosphingosine + stearoyl-CoA Homo sapiens
-
 N-stearoyldihydrosphingosine + CoA
-
 ?
additional information Homo sapiens CERS4 transfers C18 and C20 fatty acids ?
-
 ?
additional information Homo sapiens CERS5 transfers C16 fatty acids, and potentially also C14 and C18 fatty acids ?
-
 ?
additional information Homo sapiens CERS6 transfers C14 and C16 fatty acids ?
-
 ?
sphingosine + eicosanoyl-CoA Homo sapiens
-
 N-eicosanoylsphingosine + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q6ZMG9
-
-
Homo sapiens Q8N5B7
-
-
Homo sapiens Q9HA82
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydrosphingosine + myristoyl-CoA
-
 Homo sapiens N-myristoyldihydrosphingosine + CoA
-
 ?
dihydrosphingosine + palmitoyl-CoA
-
 Homo sapiens N-palmitoyldihydrosphingosine + CoA
-
 ?
dihydrosphingosine + palmitoyl-CoA best substrate Homo sapiens N-palmitoyldihydrosphingosine + CoA
-
 ?
dihydrosphingosine + stearoyl-CoA
-
 Homo sapiens N-stearoyldihydrosphingosine + CoA
-
 ?
additional information CERS4 transfers C18 and C20 fatty acids Homo sapiens ?
-
 ?
additional information CERS5 transfers C16 fatty acids, and potentially also C14 and C18 fatty acids Homo sapiens ?
-
 ?
additional information CERS6 transfers C14 and C16 fatty acids Homo sapiens ?
-
 ?
additional information CERS activity is assayed using LC-MS/MS for product quantification, crude extracts containing cell membranes are firstly prepared from tissues or cultured cells, reactions contain deuterated dihydrosphingosine (or sphingosine) and a fatty acid substrate linked to CoA (C16:0 to C24:0/24:1), detailed method descritpion and evaluation, overview Homo sapiens ?
-
 ?
sphingosine + eicosanoyl-CoA
-
 Homo sapiens N-eicosanoylsphingosine + CoA
-
 ?

Synonyms

Synonyms Comment Organism
ceramide synthase 4
-
 Homo sapiens
ceramide synthase 5
-
 Homo sapiens
ceramide synthase 6
-
 Homo sapiens
CerS4
-
 Homo sapiens
CerS5
-
 Homo sapiens
CerS6
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

General Information

General Information Comment Organism
physiological function ceramides are synthesized by ceramide synthases through the addition of a variable length fatty acid to the amine group of a sphingoid base. In mammalian cells, the sphingoid base used for de novo ceramide synthesis is usually the C18:0 lipid dihydrosphingosine. Ceramide synthesis is catalyzed by a family of six ceramide synthases (CERS1-6), each of which preferentially transfers fatty acids of different lengths to the amine group of dihydrosphingosine Homo sapiens