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Literature summary for 2.3.1.24 extracted from

  • Sassa, T.; Hirayama, T.; Kihara, A.
    Enzyme activities of the ceramide synthases CERS2-6 are regulated by phosphorylation in the C-terminal region (2016), J. Biol. Chem., 291, 7477-7487 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.24

Protein Variants

Protein Variants Comment Organism
S341A mutation in potential phosphorylation site, about 45% of wild-type activity, substrate tetracosanoyl-CoA Mus musculus
S349A mutation in potential phosphorylation site, about 90% of wild-type activity, substrate tetracosanoyl-CoA Mus musculus
T346A mutation in potential phosphorylation site, about 80% of wild-type activity, substrate tetracosanoyl-CoA Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00107
-
 sphingosine absence of lambda protein phosphatase, pH 7.4, 37°C Mus musculus
0.0077
-
 sphingosine presence of lambda protein phosphatase, pH 7.4, 37°C Mus musculus
0.0546
-
 tetracosanoyl-CoA presence of lambda protein phosphatase, pH 7.4, 37°C Mus musculus
0.0629
-
 tetracosanoyl-CoA absence of lambda protein phosphatase, pH 7.4, 37°C Mus musculus

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8IU89 isoform CerS3
-
Homo sapiens Q8N5B7 isoform CerS5
-
Homo sapiens Q96G23 isoform CerS2
-
Homo sapiens Q9HA82 isoform CerS4
-
Homo sapiens Q9NTG7 isoform CerS6
-
Mus musculus Q924Z4 isoform CerS2
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Mus musculus
glycoprotein
-
 Homo sapiens
no glycoprotein
-
 Homo sapiens
phosphoprotein isoform Cers2 is phosphorylated at the cytoplasmic C-terminal region. Treatment of cells with the casein kinase CK2-specific inhibitor CX-4945 lowers the phosphorylation level. Phosphorylation of CERS2 is important for its catalytic activity, acting mainly by increasing its Vmax value Homo sapiens
phosphoprotein isoform Cers2 is phosphorylated at the cytoplasmic C-terminalregion Mus musculus
phosphoprotein isoform Cers3 is phosphorylated at the cytoplasmic C-terminal region. Treatment of cells with the casein kinase CK2-specific inhibitor CX-4945 does not lower the phosphorylation level. Treatment with lambda protein phosphatase reduces the activity by 28% Homo sapiens
phosphoprotein isoform Cers4 is phosphorylated at the cytoplasmic C-terminal region. Treatment with lambda protein phosphatase reduces the activity by 52% Homo sapiens
phosphoprotein isoform Cers5 is phosphorylated at the cytoplasmic C-terminal region. Treatment with lambda protein phosphatase reduces the activity by 27% Homo sapiens
phosphoprotein isoform Cers6 is phosphorylated by serine kinase CK2 at the cytoplasmic C-terminal region. Treatment with lambda protein phosphatase reduces the activity by 10% Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
docosanoyl-CoA + sphingosine
-
 Homo sapiens CoA + N-docosanoylsphingosine
-
 ?
eicosanoyl-CoA + sphingosine
-
 Homo sapiens CoA + N-eicosanoylsphingosine
-
 ?
hexacosanoyl-CoA + sphingosine
-
 Homo sapiens CoA + N-hexacosanoylsphingosine
-
 ?
additional information expression of CERS3 increases the levels of C18-C22-ceramides, whereas it reduces the levels of C16:0-ceramide and C24-ceramides Homo sapiens ?
-
-
additional information expression of CERS3 increases the levels of C26:0-ceramide as well as those of C18:0-ceramide, C20:0-ceramide, and C24:0-ceramide, whereas it reduces the level of C16:0-ceramide Homo sapiens ?
-
-
additional information expression of CERS5 increases the level of C16:0-ceramide and decreases levels of C18:0-ceramide, C22:0-ceramide, and C24-ceramides Homo sapiens ?
-
-
palmitoyl-CoA + sphingosine
-
 Homo sapiens CoA + N-palmitoylsphingosine
-
 ?
palmitoyl-CoA + sphingosine
-
 Homo sapiens CoA + palmitoylsphingosine
-
 ?
stearoyl-CoA + sphingosine
-
 Homo sapiens CoA + N-stearoylsphingosine
-
 ?
tetracosanoyl-CoA + sphingosine
-
 Mus musculus CoA + N-tetracosanoylsphingosine
-
 ?
tetracosanoyl-CoA + sphingosine
-
 Homo sapiens CoA + N-tetracosanoylsphingosine
-
 ?