Literature summary for 2.3.1.24 extracted from

Shimeno, H.; Soeda, S.; Yasukouchi, M.; Okamura, N.; Nagamatsu, A.
Fatty acyl-CoA sphingosine acyltransferase in bovine brain mitochondria its solubilization and reconstitution onto the membrane lipid liposomes (1995), Biol. Pharm. Bull., 18, 1335-1339 .

Search for more literature for 2.3.1.24

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	addition of phosphatidylserine-liposomes increases the enzyme activity by 6.7%. Phospholipid-containing liposomes are potent stimulators of the enzyme, best is phosphatidylserine in liposomes, suggesting an important role of the net negative charges, overview	Bos taurus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
microsome	-	Bos taurus	-	-
mitochondrial membrane	-	Bos taurus	31966	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-sphingosine + palmitoyl-CoA	Bos taurus	-	N-palmitoyl-D-sphingosine + CoA	-	?

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	A0A3Q1MJ30	-	-

Purification (Commentary)

Purification (Comment)	Organism
solubilization of membrane-bound enzyme from brain mitochondria by 2% of Trinton X-100, followed by ammonium sulfate fractionation and dialysis	Bos taurus

Renatured (Commentary)

Renatured (Comment)	Organism
reconstitution of solubilized membrane enzyme in membrane lipid liposomes. The ability of phospholipids to enhance the activity of solubilized ceramide synthase is specific and structure-related. The most potent stimulator is phosphatidylserine in liposomes, suggesting an important role of the net negative charges	Bos taurus

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Bos taurus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0011	-	partially purified enzyme, pH 7.4, 37°C	Bos taurus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-sphingosine + palmitoyl-CoA	-	Bos taurus	N-palmitoyl-D-sphingosine + CoA	-	?

Synonyms

Synonyms	Comment	Organism
ceramide synthase	-	Bos taurus
ceramide synthase 6	-	Bos taurus
fatty acyl-CoA:sphingosine acyltransferase	-	Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Bos taurus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Bos taurus

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.5	8	over 50% ox maximal activity at pH 6.5-8.0, inactive at pH 4.5, maximally active at pH 7.5	Bos taurus

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Release 2023.1 (January 2023)