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Literature summary for 2.3.1.24 extracted from

  • Veret, J.; Coant, N.; Berdyshev, E.V.; Skobeleva, A.; Therville, N.; Bailbe, D.; Gorshkova, I.; Natarajan, V.; Portha, B.; Le Stunff, H.
    Ceramide synthase 4 and de novo production of ceramides with specific N-acyl chain lengths are involved in glucolipotoxicity-induced apoptosis of INS-1 beta-cells (2011), Biochem. J., 438, 177-189 .
    View publication on PubMed
Search for more literature for 2.3.1.24

Cloned(Commentary)

Cloned (Comment) Organism
gene CERS4, quantitative real-time PCR enzyme expression analysis, transient overexpression of HA-tagged CerS4 in INS-1 cells increasing the levels of ceramides C18:0 and C22:0 by 20%, but it has no effect on the accumulation of ceramides C16:0 and C24:0 Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sphingosine + behenoyl-CoA Rattus norvegicus
-
 N-behenoylsphingosine + CoA
-
 ?
sphingosine + stearoyl-CoA Rattus norvegicus
-
 N-stearoylsphingosine + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus D3ZU86
-
-

Source Tissue

Source Tissue Comment Organism Textmining
INS-1 cell
-
 Rattus norvegicus
-
insulinoma cell
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sphingosine + behenoyl-CoA
-
 Rattus norvegicus N-behenoylsphingosine + CoA
-
 ?
sphingosine + stearoyl-CoA
-
 Rattus norvegicus N-stearoylsphingosine + CoA
-
 ?

Synonyms

Synonyms Comment Organism
ceramide synthase 4
-
 Rattus norvegicus
CerS4
-
 Rattus norvegicus

Expression

Organism Comment Expression
Rattus norvegicus palmitate increases the levels of CerS4 mRNA 3fold in the presence of 30 mM glucose, overview up

General Information

General Information Comment Organism
physiological function CerS4 plays a critical role in beta-cells apoptosis induced by glucolipotoxicity through the synthesis of specific ceramide species such as C18:0. Glucolipotoxicity activates caspase 3/7 through ceramide accumulation in INS-1 cells. Transient overexpression of HA-tagged CerS4 increases the levels of ceramides C18:0 and C22:0 by 20%, but has no effect on the accumulation of ceramides C16:0 and C24:0. CerS4 potentiates palmitate-induced beta-cell apoptosis. Overexpression of HA-tagged CerS4 potentiates ceramide accumulation induced by stearate, which is particularly toxic for beta-cells Rattus norvegicus