General Stability | Organism |
---|---|
incubation of microsomes with 0.150 mM palmitoyl CoA affects microsomal integrity since a significant reduction in the latency of mannose-6-phosphatase is observed, addition of 0.02 mM bovine serum albumin (BSA) together with palmitoyl-CoA prevents this effect. The optimum ratio of BSA to palmitoyl-CoA is determined to be 1:3-4 | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fumonisin B1 | enzyme inhibition in vivo and in vitro | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum membrane | cytosolic surface | Rattus norvegicus | 5789 | - |
microsome | topology of dlhydroceramide synthesis in microsomes, overview | Rattus norvegicus | - |
- |
additional information | no endogenous activity is detected in the Golgi apparatus or plasma membrane fractions. Transport of (dihydro)-ceramide from the endoplasmic reticulum to sites of further metabolism in a pre-Golgi apparatus compartment and in the cis and medial cisternae of the Golgi apparatus | Rattus norvegicus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 1.5fold activation at 2 mM | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Rattus norvegicus Wistar | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-erythro-sphingosine + arachidonoyl-CoA | i.e. trans-D-erythro-2-amino-4-octadecene-1,3-diol | Rattus norvegicus | N-arachidonoyl-DL-sphingosine + CoA | - |
? | |
DL-erythro-sphingosine + arachidonoyl-CoA | i.e. trans-D-erythro-2-amino-4-octadecene-1,3-diol | Rattus norvegicus Wistar | N-arachidonoyl-DL-sphingosine + CoA | - |
? | |
DL-erythro-sphingosine + palmitoyl-CoA | i.e. trans-D-erythro-2-amino-4-octadecene-1,3-diol | Rattus norvegicus | N-palmitoyl-DL-sphingosine + CoA | - |
? | |
DL-erythro-sphingosine + palmitoyl-CoA | i.e. trans-D-erythro-2-amino-4-octadecene-1,3-diol | Rattus norvegicus Wistar | N-palmitoyl-DL-sphingosine + CoA | - |
? | |
DL-erythro-sphingosine + stearoyl-CoA | i.e. trans-D-erythro-2-amino-4-octadecene-1,3-diol | Rattus norvegicus | N-stearoyl-DL-sphingosine + CoA | - |
? | |
DL-erythro-sphingosine + stearoyl-CoA | i.e. trans-D-erythro-2-amino-4-octadecene-1,3-diol | Rattus norvegicus Wistar | N-stearoyl-DL-sphingosine + CoA | - |
? | |
sphinganine + arachidonoyl-CoA | i.e. DL-erythro-2-amino-1,3-octadecanediol | Rattus norvegicus | N-arachidonoyl-DL-dihydrosphingosine + CoA | - |
? | |
sphinganine + arachidonoyl-CoA | i.e. DL-erythro-2-amino-1,3-octadecanediol | Rattus norvegicus Wistar | N-arachidonoyl-DL-dihydrosphingosine + CoA | - |
? | |
sphinganine + palmitoyl-CoA | i.e. DL-erythro-2-amino-1,3-octadecanediol | Rattus norvegicus | N-palmitoyl-DL-dihydrosphingosine + CoA | - |
? | |
sphinganine + palmitoyl-CoA | i.e. DL-erythro-2-amino-1,3-octadecanediol | Rattus norvegicus Wistar | N-palmitoyl-DL-dihydrosphingosine + CoA | - |
? | |
sphinganine + stearoyl-CoA | i.e. DL-erythro-2-amino-1,3-octadecanediol | Rattus norvegicus | N-stearoyl-DL-dihydrosphingosine + CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
sphinganine N-acyltransferase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
metabolism | ceramide, a key intermediate in sphingolipid metabolism, is synthesized by acylation of sphinganine followed by dehydrogenation of dihydroceramide to ceramide | Rattus norvegicus |