Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.24 extracted from

  • Hirschberg, K.; Rodger, J.; Futerman, A.H.
    The long-chain sphingoid base of sphingolipids is acylated at the cytosolic surface of the endoplasmic reticulum in rat liver (1993), Biochem. J., 290, 751-757 .
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
incubation of microsomes with 0.150 mM palmitoyl CoA affects microsomal integrity since a significant reduction in the latency of mannose-6-phosphatase is observed, addition of 0.02 mM bovine serum albumin (BSA) together with palmitoyl-CoA prevents this effect. The optimum ratio of BSA to palmitoyl-CoA is determined to be 1:3-4 Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
fumonisin B1 enzyme inhibition in vivo and in vitro Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum membrane cytosolic surface Rattus norvegicus 5789
-
microsome topology of dlhydroceramide synthesis in microsomes, overview Rattus norvegicus
-
-
additional information no endogenous activity is detected in the Golgi apparatus or plasma membrane fractions. Transport of (dihydro)-ceramide from the endoplasmic reticulum to sites of further metabolism in a pre-Golgi apparatus compartment and in the cis and medial cisternae of the Golgi apparatus Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 1.5fold activation at 2 mM Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-
Rattus norvegicus Wistar
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-erythro-sphingosine + arachidonoyl-CoA i.e. trans-D-erythro-2-amino-4-octadecene-1,3-diol Rattus norvegicus N-arachidonoyl-DL-sphingosine + CoA
-
?
DL-erythro-sphingosine + arachidonoyl-CoA i.e. trans-D-erythro-2-amino-4-octadecene-1,3-diol Rattus norvegicus Wistar N-arachidonoyl-DL-sphingosine + CoA
-
?
DL-erythro-sphingosine + palmitoyl-CoA i.e. trans-D-erythro-2-amino-4-octadecene-1,3-diol Rattus norvegicus N-palmitoyl-DL-sphingosine + CoA
-
?
DL-erythro-sphingosine + palmitoyl-CoA i.e. trans-D-erythro-2-amino-4-octadecene-1,3-diol Rattus norvegicus Wistar N-palmitoyl-DL-sphingosine + CoA
-
?
DL-erythro-sphingosine + stearoyl-CoA i.e. trans-D-erythro-2-amino-4-octadecene-1,3-diol Rattus norvegicus N-stearoyl-DL-sphingosine + CoA
-
?
DL-erythro-sphingosine + stearoyl-CoA i.e. trans-D-erythro-2-amino-4-octadecene-1,3-diol Rattus norvegicus Wistar N-stearoyl-DL-sphingosine + CoA
-
?
sphinganine + arachidonoyl-CoA i.e. DL-erythro-2-amino-1,3-octadecanediol Rattus norvegicus N-arachidonoyl-DL-dihydrosphingosine + CoA
-
?
sphinganine + arachidonoyl-CoA i.e. DL-erythro-2-amino-1,3-octadecanediol Rattus norvegicus Wistar N-arachidonoyl-DL-dihydrosphingosine + CoA
-
?
sphinganine + palmitoyl-CoA i.e. DL-erythro-2-amino-1,3-octadecanediol Rattus norvegicus N-palmitoyl-DL-dihydrosphingosine + CoA
-
?
sphinganine + palmitoyl-CoA i.e. DL-erythro-2-amino-1,3-octadecanediol Rattus norvegicus Wistar N-palmitoyl-DL-dihydrosphingosine + CoA
-
?
sphinganine + stearoyl-CoA i.e. DL-erythro-2-amino-1,3-octadecanediol Rattus norvegicus N-stearoyl-DL-dihydrosphingosine + CoA
-
?

Synonyms

Synonyms Comment Organism
sphinganine N-acyltransferase
-
Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Rattus norvegicus

General Information

General Information Comment Organism
metabolism ceramide, a key intermediate in sphingolipid metabolism, is synthesized by acylation of sphinganine followed by dehydrogenation of dihydroceramide to ceramide Rattus norvegicus