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Literature summary for 2.3.1.24 extracted from

  • Basnakian, A.G.; Ueda, N.; Hong, X.; Galitovsky, V.E.; Yin, X.; Shah, S.V.
    Ceramide synthase is essential for endonuclease-mediated death of renal tubular epithelial cells induced by hypoxia-reoxygenation (2005), Am. J. Physiol., 288, F308-F314 .
    View publication on PubMed
Search for more literature for 2.3.1.24

Activating Compound

Activating Compound Comment Organism Structure
additional information exposure of cells to hypoxia-reoxygenation results in a significant increase in ceramide synthase activity Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
fumonisin B1 specific ceramide synthase inhibitor, suppresses hypoxia-reoxygenation-induced ceramide generation and provides protection against hypoxia-reoxygenation-induced EndoG release, DNA fragmentation, and cell death at 0.025-0.1 mM Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sphinganine + palmitoyl-CoA Rattus norvegicus
-
 N-palmitoyldihydrosphingosine + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
NRK-52E cell
-
 Rattus norvegicus
-
renal tubule epithelium
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sphinganine + palmitoyl-CoA
-
 Rattus norvegicus N-palmitoyldihydrosphingosine + CoA
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Rattus norvegicus

General Information

General Information Comment Organism
malfunction ceramide synthase-specific inhibitor fumonisin B1 suppresses hypoxia-reoxygenation-induced ceramide generation and provides protection against hypoxia-reoxygenation-induced EndoG release, DNA fragmentation, and cell death Rattus norvegicus
physiological function ceramide plays a role in the cell signaling pathway involved in apoptosis. Ceramide synthase is essential for endonuclease-mediated death of renal tubular epithelial cells induced by hypoxia reoxygenation. Mainly, enhanced ceramide generation is the result of hydrolysis of sphingomyelin by sphingomyelinases. Role of ceramide synthase in enhanced ceramide generation, overview. Exposure of cells to hypoxia-reoxygenation results in a significant increase in ceramide synthase activity without any significant change in acid or neutral sphingomyelinase. Ceramide synthase-dependent ceramide generation is a key modulator of EndoG-mediated cytotoxicity in hypoxia-reoxygenation injury to renal tubular epithelial cells Rattus norvegicus