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Literature summary for 2.3.1.233 extracted from

  • Funa, N.; Ohnishi, Y.; Ebizuka, Y.; Horinouchi, S.
    Alteration of reaction and substrate specificity of a bacterial type III polyketide synthase by site-directed mutagenesis (2002), Biochem. J., 367, 781-789.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.233

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptomyces griseus

Protein Variants

Protein Variants Comment Organism
A305I the mutant produces only a triketide pyrone from hexanoyl-CoA as starter substrate Streptomyces griseus
A305S the mutant produces a triketide and tetraketide pyrone from hexanoyl-CoA as starter substrate Streptomyces griseus
C138A the mutant loses the ability to decarboxylate malonyl-CoA Streptomyces griseus
C138Q the mutant loses the ability to decarboxylate malonyl-CoA Streptomyces griseus
C138S the mutation does not influence the decarboxylation of malonyl-CoA Streptomyces griseus
F188A inactive Streptomyces griseus
F188S inactive Streptomyces griseus
F188Y the mutant shows reduced catalytic efficiency with hexanoyl-CoA as substrate Streptomyces griseus
H270A inactive Streptomyces griseus
H270N the mutant produces triacetic acid lactone and flaviolin, an auto-oxidized product of 1,3,6,8-tetrahydroxynaphthalene, at a reduced rate Streptomyces griseus
H270Q the mutant retains high activity to catalyse polyketide formation, whereas the derailment products triacetic acid lactone and 6-acetonyl-4-hydroxy-2-pyrone (tetraketide lactone) are observed in increased amounts Streptomyces griseus
N303D inactive Streptomyces griseus
N303H inactive Streptomyces griseus
N303Q inactive Streptomyces griseus
Y224F the mutant is able to accept malonyl-CoA as a starter substrate Streptomyces griseus
Y224G the mutant shows reduced catalytic efficiency with hexanoyl-CoA as substrate Streptomyces griseus
Y224L the mutant shows increased catalytic efficiency with hexanoyl-CoA as substrate Streptomyces griseus
Y224W the mutant is able to accept malonyl-CoA as a starter substrate Streptomyces griseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0087
-
 hexanoyl-CoA mutant enzyme A350I, at pH 7.5 and 30°C Streptomyces griseus
0.0132
-
 hexanoyl-CoA wild type enzyme, at pH 7.5 and 30°C Streptomyces griseus
0.0143
-
 hexanoyl-CoA mutant enzyme Y224L, at pH 7.5 and 30°C Streptomyces griseus
0.0157
-
 hexanoyl-CoA mutant enzyme A350S, at pH 7.5 and 30°C Streptomyces griseus
0.0508
-
 hexanoyl-CoA mutant enzyme Y224G, at pH 7.5 and 30°C Streptomyces griseus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 x * 40000, SDS-PAGE Streptomyces griseus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5 malonyl-CoA Streptomyces griseus
-
 1,3,6,8-tetrahydroxynaphthalene + 5 CoA + 5 CO2 + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces griseus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-binding resin column chromatography Streptomyces griseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5 malonyl-CoA
-
 Streptomyces griseus 1,3,6,8-tetrahydroxynaphthalene + 5 CoA + 5 CO2 + H2O
-
 ?
hexanoyl-CoA
-
 Streptomyces griseus 4-hydroxy-6-pentyl-2-pyrone + 4-hydroxy-6-(2'-oxoheptyl)-2-pyrone + CoA + CO2 + H2O
-
 ?
phenylacetyl-CoA
-
 Streptomyces griseus 6-benzyl-4-hydroxy-2-pyrone + 4-hydroxy-6-(2'-oxo-3'-phenylpropyl)-2-pyrone + CoA + CO2 + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Streptomyces griseus

Synonyms

Synonyms Comment Organism
RppA
-
 Streptomyces griseus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.6
-
 hexanoyl-CoA wild type enzyme, at pH 7.5 and 30°C Streptomyces griseus
0.65
-
 hexanoyl-CoA mutant enzyme Y224G, at pH 7.5 and 30°C Streptomyces griseus
1.45
-
 hexanoyl-CoA mutant enzyme Y224L, at pH 7.5 and 30°C Streptomyces griseus
2.07
-
 hexanoyl-CoA mutant enzyme A350I, at pH 7.5 and 30°C Streptomyces griseus
9
-
 hexanoyl-CoA mutant enzyme A350S, at pH 7.5 and 30°C Streptomyces griseus

General Information

General Information Comment Organism
metabolism the enzyme catalyses the synthesis of 1,3,6,8-tetrahydroxynaphthalene which is the key intermediate of melanin biosynthesis Streptomyces griseus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
12.85
-
 hexanoyl-CoA mutant enzyme Y224G, at pH 7.5 and 30°C Streptomyces griseus
45.41
-
 hexanoyl-CoA wild type enzyme, at pH 7.5 and 30°C Streptomyces griseus
101.7
-
 hexanoyl-CoA mutant enzyme Y224L, at pH 7.5 and 30°C Streptomyces griseus
238.9
-
 hexanoyl-CoA mutant enzyme A350I, at pH 7.5 and 30°C Streptomyces griseus
574.7
-
 hexanoyl-CoA mutant enzyme A350S, at pH 7.5 and 30°C Streptomyces griseus