Cloned (Comment) | Organism |
---|---|
gene AtLPCAT2, recombinant overexpression in Saccharomyces cerevisiae haploid ALE1-knockout strain (BY4742, Matalpa, his3DELTA1, leu2DELTA0, lys2DELTA0, ura3DELTA0, YOR175c::kanMX4) | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | recombinant expression of Arabidopsis thaliana LPCAT2, differences in incorporation of [14C]oleoyl-CoA into PtdCho in microsomal preparations of ale1 yeast expressing AtLPCAT2 with and without DTNB, overview | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DTNB | complete inhibition of LPCAT in wild-type and in recombinant heterologous yeast microsomes | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Saccharomyces cerevisiae | - |
- |
microsome | - |
Arabidopsis thaliana | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oleoyl-CoA + 1-oleoyl-lysophosphatidic acid | Arabidopsis thaliana | - |
CoA + 1,2-dioleoyl-sn-lysophosphatidic acid | - |
r | |
oleoyl-CoA + 1-oleoyl-sn-lysophosphatidylcholine | Saccharomyces cerevisiae | - |
CoA + 1,2-dioleoyl-sn-lysophosphatidylcholine | - |
r | |
oleoyl-CoA + 1-oleoyl-sn-lysophosphatidylcholine | Arabidopsis thaliana | - |
CoA + 1,2-dioleoyl-sn-lysophosphatidylcholine | - |
r | |
oleoyl-CoA + 1-oleoyl-sn-lysophosphatidylethanolamine | Saccharomyces cerevisiae | - |
CoA + 1,2-dioleoyl-sn-lysophosphatidylethanolamine | - |
r | |
oleoyl-CoA + 1-oleoyl-sn-lysophosphatidylethanolamine | Arabidopsis thaliana | - |
CoA + 1,2-dioleoyl-sn-lysophosphatidylethanolamine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9CAN8 | - |
- |
Saccharomyces cerevisiae | Q08548 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity (towards different acyl acceptors) of the lysophospholipid:acyl-CoA acyltransferase in the forward reactions with [14C]oleoyl-CoA, assay optimization. Lysophosphatidylcholine is by far the best substrate for AtLPCAT2. Ratio between reverse and forward activities for different LPLATs for different phospholipids, overview. Highest amounts in PtdCho with AtLPCAT2 | Arabidopsis thaliana | ? | - |
- |
|
additional information | substrate specificity (towards different acyl acceptors) of the lysophospholipid:acyl-CoA acyltransferase in the forward reactions with [14C]oleoyl-CoA, assay optimization. Ratio between reverse and forward activities for different LPLATs for different phospholipids, overview. Very high activity in the forward reaction by the Ale1, while its ability to operate reversibly on all tested lipids is very low, highest amounts in PtdCho | Saccharomyces cerevisiae | ? | - |
- |
|
oleoyl-CoA + 1-oleoyl-lysophosphatidic acid | - |
Arabidopsis thaliana | CoA + 1,2-dioleoyl-sn-lysophosphatidic acid | - |
r | |
oleoyl-CoA + 1-oleoyl-lysophosphatidic acid | low activity, cf. EC 2.3.1.51 | Saccharomyces cerevisiae | CoA + 1,2-dioleoyl-sn-lysophosphatidic acid | - |
r | |
oleoyl-CoA + 1-oleoyl-lysophosphatidic acid | very low activity, cf. EC 2.3.1.51 | Arabidopsis thaliana | CoA + 1,2-dioleoyl-sn-lysophosphatidic acid | - |
r | |
oleoyl-CoA + 1-oleoyl-sn-lysophosphatidylcholine | - |
Saccharomyces cerevisiae | CoA + 1,2-dioleoyl-sn-lysophosphatidylcholine | - |
r | |
oleoyl-CoA + 1-oleoyl-sn-lysophosphatidylcholine | - |
Arabidopsis thaliana | CoA + 1,2-dioleoyl-sn-lysophosphatidylcholine | - |
r | |
oleoyl-CoA + 1-oleoyl-sn-lysophosphatidylcholine | preferred substrate, high activity | Saccharomyces cerevisiae | CoA + 1,2-dioleoyl-sn-lysophosphatidylcholine | - |
r | |
oleoyl-CoA + 1-oleoyl-sn-lysophosphatidylcholine | preferred substrate, high activity | Arabidopsis thaliana | CoA + 1,2-dioleoyl-sn-lysophosphatidylcholine | - |
r | |
oleoyl-CoA + 1-oleoyl-sn-lysophosphatidylethanolamine | - |
Saccharomyces cerevisiae | CoA + 1,2-dioleoyl-sn-lysophosphatidylethanolamine | - |
r | |
oleoyl-CoA + 1-oleoyl-sn-lysophosphatidylethanolamine | - |
Arabidopsis thaliana | CoA + 1,2-dioleoyl-sn-lysophosphatidylethanolamine | - |
r | |
oleoyl-CoA + 1-oleoyl-sn-lysophosphatidylethanolamine | low activity, cf. EC 2.3.1.121 | Saccharomyces cerevisiae | CoA + 1,2-dioleoyl-sn-lysophosphatidylethanolamine | - |
r | |
oleoyl-CoA + 1-oleoyl-sn-lysophosphatidylethanolamine | very low activity, cf. EC 2.3.1.121 | Arabidopsis thaliana | CoA + 1,2-dioleoyl-sn-lysophosphatidylethanolamine | - |
r |
Synonyms | Comment | Organism |
---|---|---|
ALE1 | - |
Saccharomyces cerevisiae |
AtLPLAT2 | - |
Arabidopsis thaliana |
LPCAT2 | - |
Arabidopsis thaliana |
LPLAT | - |
Saccharomyces cerevisiae |
LPLAT | - |
Arabidopsis thaliana |
lysophospholipid acyltransferase | - |
Saccharomyces cerevisiae |
lysophospholipid acyltransferase | - |
Arabidopsis thaliana |
lysophospholipid:acyl-CoA acyltransferase | - |
Saccharomyces cerevisiae |
lysophospholipid:acyl-CoA acyltransferase | - |
Arabidopsis thaliana |
More | see also EC 2.3.1.121 and EC 2.3.1.51 | Saccharomyces cerevisiae |
More | see also EC 2.3.1.121 and EC 2.3.1.51 | Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
30 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Saccharomyces cerevisiae |
7.2 | - |
assay at | Arabidopsis thaliana |