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Literature summary for 2.3.1.23 extracted from

  • Du, X.; Hu, J.; Zhang, Q.; Liu, Q.; Xiang, X.; Dong, J.; Lou, B.; He, S.; Gu, X.; Cao, Y.; Li, Y.; Ding, T.
    A novel assay for measuring recombinant human lysophosphatidylcholine acyltransferase 3 activity (2019), FEBS open bio, 9, 1734-1743 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine LPCAT3 may have potential as a therapeutic target for diseases, including atherosclerosis, non-alcoholic steatohepatitis, and carcinoma Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
gene LPCAT3, recombinant expression of C-terminally Twin-Strep-tagged enzyme in Spodoptera frugiperda Sf9 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
TSI-10 an LPCAT3 inhibitor Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km of NBD-lyso-PC and arachidonoyl CoA by the model of Bi-Bi reaction under optimal conditions. The theoretical Km values calculated by Bi-Bi kinetic equations are more accurate than that calculated by the Michaelis-Menten equation because the apparent Km of one substrate calculated by the Michaelis-Menten equation may be influenced by the concentration of another, whereas Bi-Bi kinetic equations could overcome this disadvantage. From the Lineweaver-Burk plots, a sequential kinetic mechanism can be proposed, but only a sequential Bi-Bi kinetic mechanism can be infered Homo sapiens
0.011
-
Arachidonoyl-CoA pH 6.0, 30°C, recombinant enzyme Homo sapiens
0.2668
-
NBD-labelled lysophosphatidylcholine pH 6.0, 30°C, recombinant enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Homo sapiens 16020
-

Organism

Organism UniProt Comment Textmining
Homo sapiens Q6P1A2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Twin-Strep-tagged enzyme from Sf9 insect cells by membrane preparation, affinity chromatography, and gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonoyl-CoA + NBD-labelled lysophosphatidylcholine
-
Homo sapiens CoA + NBD-2-arachidonoyl-phosphatidylcholine
-
-

Synonyms

Synonyms Comment Organism
LPCAT3
-
Homo sapiens
LPLAT5
-
Homo sapiens
lysophosphatidylcholine acyltransferase 3
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.174
-
pH 6.0, 30°C, recombinant enzyme Homo sapiens TSI-10

General Information

General Information Comment Organism
physiological function lysophosphatidylcholine acyltransferase 3 (LPCAT3) is an important enzyme in phospholipid remodeling, a process that influences the biophysical properties of cell membranes and thus cell function. LPCAT3 is involved in several diseases, including atherosclerosis, non-alcoholic steatohepatitis, and carcinoma Homo sapiens