Cloned (Comment) | Organism |
---|---|
gene At1g78690, recombinant expression of His-tagged enzyme in Escherichia coli strain BLR(DE3)pLysS | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1-acyl-lysophosphatidylethanolamine | Arabidopsis thaliana | - |
CoA + 1,2-diacyl-lysophosphatidylethanolamine | - |
? | |
acyl-CoA + 1-acyl-lysophosphatidylglycerol | Arabidopsis thaliana | - |
CoA + 1,2-diacyl-lysophosphatidylglycerol | - |
? | |
acyl-CoA + 1-acyl-lysophosphatidylinositol | Arabidopsis thaliana | - |
CoA + 1,2-diacyl-lysophosphatidylinositol | - |
? | |
acyl-CoA + 1-acyl-lysophosphatidylserine | Arabidopsis thaliana | - |
CoA + 1,2-diacyl-lysophosphatidylserine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9ZV87 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1-acyl-lysophosphatidylethanolamine | - |
Arabidopsis thaliana | CoA + 1,2-diacyl-lysophosphatidylethanolamine | - |
? | |
acyl-CoA + 1-acyl-lysophosphatidylglycerol | - |
Arabidopsis thaliana | CoA + 1,2-diacyl-lysophosphatidylglycerol | - |
? | |
acyl-CoA + 1-acyl-lysophosphatidylinositol | - |
Arabidopsis thaliana | CoA + 1,2-diacyl-lysophosphatidylinositol | - |
? | |
acyl-CoA + 1-acyl-lysophosphatidylserine | - |
Arabidopsis thaliana | CoA + 1,2-diacyl-lysophosphatidylserine | - |
? | |
additional information | Arabidopsis thaliana lysophospholipid acyltransferase utilizes both mono- and dilysocardiolipin as an acyl acceptor. The enzyme robustly acylates 1-acyl-lysophosphatidylethanolamine (PE), 1-acyl-lysophosphatidylglycerol (PG), 1-acyl lysophosphatidylserine (PS), and 1-acyl-phosphatidylinositol (PI) using acyl-CoA as the acyl donor to produce PE, PG, PS, and PI, respectively. Di-linoleoylphosphatidylcholine is used as the acyl donor and mono-lysocardiolipin is used as the acyl acceptor in a reaction. No transfer of the linoleoyl chains is detected in an At1g78690-dependent manner suggesting that, despite the strong homology, the cardiolipin remodeling enzyme tafazzin and the lysophospholipid acyltransferase catalyze unique reactions. No 18:2 acyl chains are detected in other phospholipid pools in an At1g78690-dependent manner. Enzyme At1g78690 does not function as a transacylase using phosphatidyylcholine as an acyl donor. Mass spectrometric identification of in vitro products | Arabidopsis thaliana | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
At1g78690 | - |
Arabidopsis thaliana |
lysophospholipid acyltransferase | - |
Arabidopsis thaliana |
N-acylphosphatidylethanolamine synthase | UniProt | Arabidopsis thaliana |
NAPE synthase | UniProt | Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
evolution | enzyme At1g78690 shares high homology (about 40%) with the cardiolipin remodeling enzyme tafazzin, but the cardiolipin remodeling enzyme tafazzin and the lysophospholipid acyltransferase catalyze unique reactions | Arabidopsis thaliana |
malfunction | despite di-acylate phosphatidylglycerol being a substrate, overexpression of At1g78690 in Escherichia coli leads to the accumulation of acyl-PG. Cardiolipin also accumulates in cells overexpressing At1g78690, cardiolipin is found in the inner mitochondrial membrane in eukaryotes and is critical for mitochondrial function. It serves as a necessary proton trap for oxidative phosphorylation and is a trigger for apoptosis | Arabidopsis thaliana |