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Literature summary for 2.3.1.23 extracted from
- Lysophosphatidylcholine acyltransferase 1 (LPCAT1) specifically interacts with phospholipid transfer protein starD10 to facilitate surfactant phospholipid trafficking in alveolar type II cells (2015), J. Biol. Chem., 290, 18559-18574.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene LPCAT1, recombinant expression of FLAG- and HA-tagged full-length and truncated enzyme in HEK-293 cell microsomes, recombinant coexpression of His-tagged or GST-tagged enzyme with His-tagged StarD10 protein in Escherichia coli | Mus musculus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Mus musculus | 5783 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine | Mus musculus | - |
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine | - |
? |  |
| acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine | Mus musculus FVB/N | - |
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine | - |
? | |
| additional information | Mus musculus | enzyme LPCAT1 directly interacts with StarD10 protein, amino acids 79-271 of LPCAT1 and the steroidogenic acute regulatory protein-related lipid transfer (START) domain of START domain-containing protein 10 (StarD10) are sufficient for this interaction. The enzyme also interacts with StarD7-I but not StarD7-II or StarD2/PCTP transfer protein | ? | - |
? | |
| additional information | Mus musculus FVB/N | enzyme LPCAT1 directly interacts with StarD10 protein, amino acids 79-271 of LPCAT1 and the steroidogenic acute regulatory protein-related lipid transfer (START) domain of START domain-containing protein 10 (StarD10) are sufficient for this interaction. The enzyme also interacts with StarD7-I but not StarD7-II or StarD2/PCTP transfer protein | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | Q3TFD2 | - |
- |
| Mus musculus FVB/N | Q3TFD2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| copurification of His-tagged or GST-tagged enzyme with His-tagged StarD10 protein from Escherichia coli by affinity chromatography | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| alveolar type II cell | - |
Mus musculus | - |
| epithelial cell | - |
Mus musculus | - |
| liver | - |
Mus musculus | - |
| lung | - |
Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine | - |
Mus musculus | CoA + 1,2-diacyl-sn-glycero-3-phosphocholine | - |
? | |
| acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine | specificity of enzyme LPCAT1 for lyso-PC as an acyl acceptor | Mus musculus | CoA + 1,2-diacyl-sn-glycero-3-phosphocholine | - |
? | |
| acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine | - |
Mus musculus FVB/N | CoA + 1,2-diacyl-sn-glycero-3-phosphocholine | - |
? | |
| acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine | specificity of enzyme LPCAT1 for lyso-PC as an acyl acceptor | Mus musculus FVB/N | CoA + 1,2-diacyl-sn-glycero-3-phosphocholine | - |
? | |
| additional information | enzyme LPCAT1 directly interacts with StarD10 protein, amino acids 79-271 of LPCAT1 and the steroidogenic acute regulatory protein-related lipid transfer (START) domain of START domain-containing protein 10 (StarD10) are sufficient for this interaction. The enzyme also interacts with StarD7-I but not StarD7-II or StarD2/PCTP transfer protein | Mus musculus | ? | - |
? | |
| additional information | enzyme LPCAT1 directly interacts with StarD10 protein, amino acids 79-271 of LPCAT1 and the steroidogenic acute regulatory protein-related lipid transfer (START) domain of START domain-containing protein 10 (StarD10) are sufficient for this interaction. The enzyme also interacts with StarD7-I but not StarD7-II or StarD2/PCTP transfer protein | Mus musculus FVB/N | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LPCAT1 | - |
Mus musculus |
| lyso-PC acyltransferase 1 | - |
Mus musculus |
| lysophosphatidylcholine acyltransferase 1 | - |
Mus musculus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | pulmonary surfactant, a mixture of proteins and phospholipids, plays an important role in facilitating gas exchange by maintaining alveolar stability. Saturated phosphatidylcholine, the major component of surfactant, is synthesized both de novo and by the remodeling of unsaturated phosphatidylcholine by lyso-PC acyltransferase 1 (LPCAT1). After synthesis in the endoplasmic reticulum, saturated phosphatidylcholine is routed to lamellar bodies for storage prior to secretion. The enzyme forms a transient complex with saturated phosphatidylcholine and specific phospholipid transport protein(s) to initiate trafficking of saturated phosphatidylcholine from the endoplasmic reticulum to the lamellar bodies | Mus musculus |
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