Cloned (Comment) | Organism |
---|---|
gene LPCAT3, real-time PCR enzyme expression analysis | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme LPCAT3 gene silencing by siRNA | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
T0901317 | a liver X recpeotr agonist | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-bound | Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine | Homo sapiens | - |
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q6P1A2 | gene LPCAT3; gene LPCAT3 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293 cell | - |
Homo sapiens | - |
liver | - |
Homo sapiens | - |
macrophage | primary peripheral blood macrophages | Homo sapiens | - |
THP-1 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine | - |
Homo sapiens | CoA + 1,2-diacyl-sn-glycero-3-phosphocholine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LPCAT3 | - |
Homo sapiens |
lysophosphatidylcholine acyltransferase 3 | - |
Homo sapiens |
membrane-bound O-acyltransferase 5 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | enzyme LPCAT3 is upregulated by liver X receptor LXR agonists, e.g. T0901317, in human macrophages | up |
General Information | Comment | Organism |
---|---|---|
malfunction | the liver X receptor-mediated effects on arachidonic acid distribution are abolished by LPCAT3 silencing, and a redistribution of arachidonic acid toward the neutral lipid fraction is observed | Homo sapiens |
metabolism | the liver X receptor-mediated induction of enzyme LPCAT3 specifically regulates the metabolism of arachidonic acid, the precursor of key inflammatory mediators, such as eicosanoids. Liver X receptor and lysophosphatidylcholine acyltransferase 3 modulate the fatty acid composition of human macrophages, overview | Homo sapiens |
physiological function | the enzyme is involved in the Lands cycle, it controls the fatty acid composition at the sn-2 position of glycerophospholipids and the availability of fatty acids, such as arachidonic acid, used for eicosanoid synthesis. LPCAT3 gene expression is regulated by liver X receptor in human primary macrophages and monocytes. Liver X receptor stimulation significantly enhances the lysophospholipid acyltransferase activity of LPCAT3 increasing the arachidonic acid content in the polar lipid fraction, specifically in phosphatidylcholines. Preconditioning of human macrophages by LXR agonist treatment increases the release of arachidonate-derived eicosanoids, such as prostaglandin E2 and thromboxane after lipopolysaccharide stimulation, with a significant attenuation by LPCAT3 silencing | Homo sapiens |