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Literature summary for 2.3.1.23 extracted from

  • Ishibashi, M.; Varin, A.; Filomenko, R.; Lopez, T.; Athias, A.; Gambert, P.; Blache, D.; Thomas, C.; Gautier, T.; Lagrost, L.; Masson, D.
    Liver X receptor regulates arachidonic acid distribution and eicosanoid release in human macrophages: a key role for lysophosphatidylcholine acyltransferase 3 (2013), Arterioscler. Thromb. Vasc. Biol., 33, 1171-1179.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene LPCAT3, real-time PCR enzyme expression analysis Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information enzyme LPCAT3 gene silencing by siRNA Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
T0901317 a liver X recpeotr agonist Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane-bound Homo sapiens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine Homo sapiens
-
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q6P1A2 gene LPCAT3; gene LPCAT3
-

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
Homo sapiens
-
liver
-
Homo sapiens
-
macrophage primary peripheral blood macrophages Homo sapiens
-
THP-1 cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
-
Homo sapiens CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
?

Synonyms

Synonyms Comment Organism
LPCAT3
-
Homo sapiens
lysophosphatidylcholine acyltransferase 3
-
Homo sapiens
membrane-bound O-acyltransferase 5
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens

Expression

Organism Comment Expression
Homo sapiens enzyme LPCAT3 is upregulated by liver X receptor LXR agonists, e.g. T0901317, in human macrophages up

General Information

General Information Comment Organism
malfunction the liver X receptor-mediated effects on arachidonic acid distribution are abolished by LPCAT3 silencing, and a redistribution of arachidonic acid toward the neutral lipid fraction is observed Homo sapiens
metabolism the liver X receptor-mediated induction of enzyme LPCAT3 specifically regulates the metabolism of arachidonic acid, the precursor of key inflammatory mediators, such as eicosanoids. Liver X receptor and lysophosphatidylcholine acyltransferase 3 modulate the fatty acid composition of human macrophages, overview Homo sapiens
physiological function the enzyme is involved in the Lands cycle, it controls the fatty acid composition at the sn-2 position of glycerophospholipids and the availability of fatty acids, such as arachidonic acid, used for eicosanoid synthesis. LPCAT3 gene expression is regulated by liver X receptor in human primary macrophages and monocytes. Liver X receptor stimulation significantly enhances the lysophospholipid acyltransferase activity of LPCAT3 increasing the arachidonic acid content in the polar lipid fraction, specifically in phosphatidylcholines. Preconditioning of human macrophages by LXR agonist treatment increases the release of arachidonate-derived eicosanoids, such as prostaglandin E2 and thromboxane after lipopolysaccharide stimulation, with a significant attenuation by LPCAT3 silencing Homo sapiens