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Literature summary for 2.3.1.225 extracted from

  • Veit, M.; Sachs, K.; Heckelmann, M.; Maretzki, D.; Hofmann, K. P.; Schmidt, M. F. G.
    Palmitoylation of rhodopsin with S-protein acyltransferase: enzyme catalyzed reaction versus autocatalytic acylation (1998), Biochim. Biophys. Acta, 1394, 90-98.
    View publication on PubMed
Search for more literature for 2.3.1.225

Inhibitors

Inhibitors Comment Organism Structure
myristoyl-CoA
-
 Bos taurus
stearoyl-CoA
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics and substrate specificity of PAT, overview Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
palmitoyl-CoA + [protein]-L-cysteine Bos taurus
-
 [protein]-S-palmitoyl-L-cysteine + CoA
-
 r
palmitoyl-CoA + [rhodopsin]-L-cysteine Bos taurus PAT incorporates fatty acid into rhodopsin with higher efficiency, 10times higher initial rate, as compared to autoacylation, presence of deacylated, free cysteine residues in dark-adapted rhodopsin increases palmitoylation via PAT [rhodopsin]-S-palmitoyl-L-cysteine + CoA
-
 r

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially, from placental membranes by anion exchange chromatography Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
placenta membrane Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Kinetics and substrate specificity of PAT, overview Bos taurus ?
-
 ?
palmitoyl-CoA + [protein]-L-cysteine
-
 Bos taurus [protein]-S-palmitoyl-L-cysteine + CoA
-
 r
palmitoyl-CoA + [rhodopsin]-L-cysteine PAT incorporates fatty acid into rhodopsin with higher efficiency, 10times higher initial rate, as compared to autoacylation, presence of deacylated, free cysteine residues in dark-adapted rhodopsin increases palmitoylation via PAT Bos taurus [rhodopsin]-S-palmitoyl-L-cysteine + CoA
-
 r
palmitoyl-CoA + [rhodopsin]-L-cysteine PAT incorporates fatty acid into rhodopsin with higher efficiency, 10times higher initial rate, as compared to autoacylation, presence of deacylated, free cysteine residues in dark-adapted rhodopsin increases palmitoylation via PAT. Rhodopsin containing rod outer segment membranes prepared from fresh, dark-adapted bovine retinae Bos taurus [rhodopsin]-S-palmitoyl-L-cysteine + CoA
-
 r
stearoyl-CoA + [rhodopsin]-L-cysteine Rhodopsin containing rod outer segment membranes prepared from fresh, dark-adapted bovine retinae Bos taurus [rhodopsin]-S-stearoyl-L-cysteine + CoA
-
 r

Synonyms

Synonyms Comment Organism
Pat
-
 Bos taurus
S-protein acyltransferase
-
 Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 purified enzyme, 30 min, stable up to Bos taurus
35
-
 about, purufued enzyme, 30 min, 50% activity remaining Bos taurus
60
-
 purified enzyme, 30 min, inactivation Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Bos taurus