Inhibitors | Comment | Organism | Structure |
---|---|---|---|
myristoyl-CoA | - |
Bos taurus | |
stearoyl-CoA | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics and substrate specificity of PAT, overview | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + [protein]-L-cysteine | Bos taurus | - |
[protein]-S-palmitoyl-L-cysteine + CoA | - |
r | |
palmitoyl-CoA + [rhodopsin]-L-cysteine | Bos taurus | PAT incorporates fatty acid into rhodopsin with higher efficiency, 10times higher initial rate, as compared to autoacylation, presence of deacylated, free cysteine residues in dark-adapted rhodopsin increases palmitoylation via PAT | [rhodopsin]-S-palmitoyl-L-cysteine + CoA | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partially, from placental membranes by anion exchange chromatography | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
placenta | membrane | Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Kinetics and substrate specificity of PAT, overview | Bos taurus | ? | - |
? | |
palmitoyl-CoA + [protein]-L-cysteine | - |
Bos taurus | [protein]-S-palmitoyl-L-cysteine + CoA | - |
r | |
palmitoyl-CoA + [rhodopsin]-L-cysteine | PAT incorporates fatty acid into rhodopsin with higher efficiency, 10times higher initial rate, as compared to autoacylation, presence of deacylated, free cysteine residues in dark-adapted rhodopsin increases palmitoylation via PAT | Bos taurus | [rhodopsin]-S-palmitoyl-L-cysteine + CoA | - |
r | |
palmitoyl-CoA + [rhodopsin]-L-cysteine | PAT incorporates fatty acid into rhodopsin with higher efficiency, 10times higher initial rate, as compared to autoacylation, presence of deacylated, free cysteine residues in dark-adapted rhodopsin increases palmitoylation via PAT. Rhodopsin containing rod outer segment membranes prepared from fresh, dark-adapted bovine retinae | Bos taurus | [rhodopsin]-S-palmitoyl-L-cysteine + CoA | - |
r | |
stearoyl-CoA + [rhodopsin]-L-cysteine | Rhodopsin containing rod outer segment membranes prepared from fresh, dark-adapted bovine retinae | Bos taurus | [rhodopsin]-S-stearoyl-L-cysteine + CoA | - |
r |
Synonyms | Comment | Organism |
---|---|---|
Pat | - |
Bos taurus |
S-protein acyltransferase | - |
Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
purified enzyme, 30 min, stable up to | Bos taurus |
35 | - |
about, purufued enzyme, 30 min, 50% activity remaining | Bos taurus |
60 | - |
purified enzyme, 30 min, inactivation | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Bos taurus |