Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.222 extracted from

  • Erbilgin, O.; Sutter, M.; Kerfeld, C.A.
    The structural basis of coenzyme A recycling in a bacterial organelle (2016), PLoS Biol., 14, e1002399 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.222

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Rhodopseudomonas palustris
expression in Escherichia coli BL21(DE3) Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals are obtained from sitting drop experiments at 22°C. High-resolution PduL crystal structures with bound substrates. The PduL fold is unrelated to that of Pta. It contains a dimetal active site involved in a catalytic mechanism distinct from that of the housekeeping phosphotransacylase (PTAC) Rhodopseudomonas palustris

Organism

Organism UniProt Comment Textmining
Rhodopseudomonas palustris Q21A54
-
-
Rhodopseudomonas palustris BisB18 Q21A54
-
-
Salmonella enterica subsp. enterica serovar Typhimurium Q9XDN5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodopseudomonas palustris
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
propanoyl-CoA + phosphate
-
 Rhodopseudomonas palustris CoA + propanoyl phosphate
-
 ?
propanoyl-CoA + phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium CoA + propanoyl phosphate
-
 ?
propanoyl-CoA + phosphate
-
 Rhodopseudomonas palustris BisB18 CoA + propanoyl phosphate
-
 ?

Synonyms

Synonyms Comment Organism
PduL
-
 Rhodopseudomonas palustris
PduL
-
 Salmonella enterica subsp. enterica serovar Typhimurium

General Information

General Information Comment Organism
evolution the phosphotransacylase (PTAC) predominantly associated with metabolosomes (PduL) has no sequence homology to the PTAC ubiquitous among fermentative bacteria (Pta). PduL and Pta exemplify functional, but not structural, convergent evolution Rhodopseudomonas palustris
evolution the phosphotransacylase (PTAC) predominantly associated with metabolosomes (PduL) has no sequence homology to the PTAC ubiquitous among fermentative bacteria (Pta). PduL and Pta exemplify functional, but not structural, convergent evolution Salmonella enterica subsp. enterica serovar Typhimurium
metabolism the fact that PduL is confined almost exclusively to metabolosomes can be used to develop an inhibitor that blocks only PduL and not Pta as a way to selectively disrupt bacterial microcompartments(BMC)-based metabolism, while not affecting most commensal organisms that require phosphotransacylase (PTAC) activity Rhodopseudomonas palustris
metabolism the fact that PduL is confined almost exclusively to metabolosomes can be used to develop an inhibitor that blocks only PduL and not Pta as a way to selectively disrupt bacterial microcompartments(BMC)-based metabolism, while not affecting most commensal organisms that require phosphotransacylase (PTAC) activity Salmonella enterica subsp. enterica serovar Typhimurium