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Literature summary for 2.3.1.222 extracted from

  • Breitkopf, R.; Uhlig, R.; Drenckhan, T.; Fischer, R.J.
    Two propanediol utilization-like proteins of Moorella thermoacetica with phosphotransacetylase activity (2016), Extremophiles, 20, 653-661 .
    View publication on PubMed
Search for more literature for 2.3.1.222

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Neomoorella thermoacetica

Inhibitors

Inhibitors Comment Organism Structure
Mn2+ 0.1 microM, 82% residual activity; 0.2 microM, 40% residual activity Neomoorella thermoacetica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 butyryl-CoA pH 7.6, 55°C Neomoorella thermoacetica
0.02
-
 butanoyl-CoA pH 7.6, 55°C Neomoorella thermoacetica
0.04
-
 acetyl-CoA pH 7.6, 55°C Neomoorella thermoacetica
0.04
-
 propanoyl-CoA pH 7.6, 55°C Neomoorella thermoacetica
0.04
-
 propionyl-CoA pH 7.6, 55°C Neomoorella thermoacetica
0.12
-
 propanoyl-CoA pH 7.6, 55°C Neomoorella thermoacetica
0.12
-
 propionyl-CoA pH 7.6, 55°C Neomoorella thermoacetica
0.4
-
 acetyl phosphate pH 7.4, 55°C Neomoorella thermoacetica
0.49
-
 acetyl-CoA pH 7.6, 55°C Neomoorella thermoacetica

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 0.0003 mM, 29% inhibition Neomoorella thermoacetica
Mn2+ 0.0003 mM, 84% inhibition Neomoorella thermoacetica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 SDS-PAGE Neomoorella thermoacetica
90000
-
 gel filtration Neomoorella thermoacetica

Organism

Organism UniProt Comment Textmining
Neomoorella thermoacetica Q2RK58
-
-
Neomoorella thermoacetica Q2RK58 isoform Pdul2
-
Neomoorella thermoacetica Q2RJ94 isoform Pdul1
-
Neomoorella thermoacetica Q2RJ94
-
-
Neomoorella thermoacetica ATCC 39073 Q2RK58
-
-
Neomoorella thermoacetica ATCC 39073 Q2RK58 isoform Pdul2
-
Neomoorella thermoacetica ATCC 39073 Q2RJ94 isoform Pdul1
-
Neomoorella thermoacetica ATCC 39073 Q2RJ94
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Neomoorella thermoacetica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + phosphate
-
 Neomoorella thermoacetica CoA + acetyl phosphate
-
 r
acetyl-CoA + phosphate reaction of EC 2.3.1.8 Neomoorella thermoacetica CoA + acetyl phosphate
-
 r
acetyl-CoA + phosphate no activity with acetyl phosphate in the reverse direction Neomoorella thermoacetica CoA + acetyl phosphate
-
 ir
acetyl-CoA + phosphate reaction of EC 2.3.1.8 Neomoorella thermoacetica CoA + acetyl phosphate
-
 ir
acetyl-CoA + phosphate
-
 Neomoorella thermoacetica ATCC 39073 CoA + acetyl phosphate
-
 r
acetyl-CoA + phosphate reaction of EC 2.3.1.8 Neomoorella thermoacetica ATCC 39073 CoA + acetyl phosphate
-
 r
acetyl-CoA + phosphate no activity with acetyl phosphate in the reverse direction Neomoorella thermoacetica ATCC 39073 CoA + acetyl phosphate
-
 ir
butanoyl-CoA + phosphate reaction of EC 2.3.1.19 Neomoorella thermoacetica CoA + butanoyl phosphate
-
 ?
butanoyl-CoA + phosphate reaction of EC 2.3.1.19 Neomoorella thermoacetica ATCC 39073 CoA + butanoyl phosphate
-
 ?
butyryl-CoA + phosphate
-
 Neomoorella thermoacetica CoA + butyryl phosphate
-
 r
butyryl-CoA + phosphate
-
 Neomoorella thermoacetica ATCC 39073 CoA + butyryl phosphate
-
 r
CoA + acetyl phosphate
-
 Neomoorella thermoacetica acetyl-CoA + phosphate
-
 r
CoA + acetyl phosphate
-
 Neomoorella thermoacetica ATCC 39073 acetyl-CoA + phosphate
-
 r
CoA + butyryl phosphate
-
 Neomoorella thermoacetica butyryl-CoA + phosphate
-
 r
CoA + propanoyl phosphate
-
 Neomoorella thermoacetica propanoyl-CoA + phosphate
-
 r
CoA + propanoyl phosphate
-
 Neomoorella thermoacetica ATCC 39073 propanoyl-CoA + phosphate
-
 r
additional information no activity with butyryl phosphate Neomoorella thermoacetica ?
-
 ?
additional information no substrates: butanoyl-CoA, acetyl phosphate Neomoorella thermoacetica ?
-
 ?
propanoyl-CoA + phosphate
-
 Neomoorella thermoacetica CoA + propanoyl phosphate
-
 r
propanoyl-CoA + phosphate
-
 Neomoorella thermoacetica ATCC 39073 CoA + propanoyl phosphate
-
 r
propionyl-CoA + phosphate
-
 Neomoorella thermoacetica CoA + propionyl phosphate
-
 ?
propionyl-CoA + phosphate
-
 Neomoorella thermoacetica ATCC 39073 CoA + propionyl phosphate
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 23000, SDS-PAGE Neomoorella thermoacetica
tetramer 4 * 23000, SDS-PAGE Neomoorella thermoacetica

Synonyms

Synonyms Comment Organism
Moth_0864
-
 Neomoorella thermoacetica
Moth_1181
-
 Neomoorella thermoacetica
pduL1
-
 Neomoorella thermoacetica
pduL2
-
 Neomoorella thermoacetica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Neomoorella thermoacetica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
 butyryl-CoA pH 7.6, 55°C Neomoorella thermoacetica
0.12
-
 butanoyl-CoA pH 7.6, 55°C Neomoorella thermoacetica
0.2
-
 acetyl-CoA pH 7.6, 55°C Neomoorella thermoacetica
0.21
-
 propanoyl-CoA pH 7.6, 55°C Neomoorella thermoacetica
0.21
-
 propionyl-CoA pH 7.6, 55°C Neomoorella thermoacetica
1.14
-
 propanoyl-CoA pH 7.6, 55°C Neomoorella thermoacetica
1.14
-
 propionyl-CoA pH 7.6, 55°C Neomoorella thermoacetica
2.65
-
 acetyl phosphate pH 7.4, 55°C Neomoorella thermoacetica
5.18
-
 acetyl-CoA pH 7.6, 55°C Neomoorella thermoacetica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
 Neomoorella thermoacetica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.67
-
 acetyl-CoA pH 7.6, 55°C Neomoorella thermoacetica
4.69
-
 acetyl-CoA pH 7.6, 55°C Neomoorella thermoacetica
5.03
-
 propanoyl-CoA pH 7.6, 55°C Neomoorella thermoacetica
5.03
-
 propionyl-CoA pH 7.6, 55°C Neomoorella thermoacetica
6.56
-
 acetyl phosphate pH 7.4, 55°C Neomoorella thermoacetica
6.68
-
 butyryl-CoA pH 7.6, 55°C Neomoorella thermoacetica
6.68
-
 butanoyl-CoA pH 7.6, 55°C Neomoorella thermoacetica
9.26
-
 propanoyl-CoA pH 7.6, 55°C Neomoorella thermoacetica
9.26
-
 propionyl-CoA pH 7.6, 55°C Neomoorella thermoacetica
10.67
-
 acetyl-CoA pH 7.6, 55°C Neomoorella thermoacetica