Any feedback?
Literature summary for 2.3.1.22 extracted from
- Novel acyl-coenzyme A:monoacylglycerol acyltransferase (MGAT) plays an important role in hepatic triacylglycerol secretion (2010), J. Lipid Res., 51, 1424-1431.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| PCR-amplification, transfection and expression of CHO cells with FLAG-tagged enzyme cDNA plasmid | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | knockdown of hepatic lysophosphatidylglycerol acyltransferase 1 gene expression in transfected CHO cells by an adenovirus construct of short hairpin RNA (shRNA) | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| shRNA-adenovirus | short hairpin RNA adenovirus construct to knockdown mouse lysophosphatidylglycerol acyltransferase 1, injected via tail vein | Mus musculus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
sequence analysis, 370 amino acids | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-acyl-sn-glycerol + acetyl-CoA | Mus musculus | - |
diacylglycerol + CoA | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
7 weeks old male diabetic (db/db) and control (db/m) mice, 7 days adjusted | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Mus musculus | - |
| intestine | - |
Mus musculus | - |
| kidney | - |
Mus musculus | - |
| liver | most highly expressed, used for experiments | Mus musculus | - |
| skin | - |
Mus musculus | - |
| thymus | - |
Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-acyl-sn-glycerol + acetyl-CoA | - |
Mus musculus | diacylglycerol + CoA | - |
? | |
| 2-acyl-sn-glycerol + palmitoyl-CoA | in vitro assay in lysate of transfected CHO cells or of microsome preparation of mouse liver, 2-monoacylglycerol is preferred over 1-monoacylglycerol, 25°C, pH 6.4 | Mus musculus | diacylglycerol + CoA | - |
? | |
| additional information | no significant production of phosphatidic acid (substrate lysophosphatidic acid), triacylglycerol (diacylglycerol), or cholesterol ester (substrate cholesterol), 25°C, pH 6.4 | Mus musculus | ? | - |
? | |
| rac-1-monoacylglycerol + palmitoyl-CoA | in vitro assay in lysate of transfected CHO cells, 25°C, pH 6.4 | Mus musculus | diacylglycerol + CoA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acyl-coenzyme A:monoacylglycerol acyltransferase | - |
Mus musculus |
| LPGAT1 | two splice isoforms | Mus musculus |
| lysophosphatidylglycerol acyltransferase 1 | - |
Mus musculus |
| MGAT | - |
Mus musculus |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| additional information | - |
infection with 60000000000 particles per animal (dose limit of no effect of control particles) leads to 83% decreased mRNA levels on day 5, 92% decrease on day 16 post-injection, associated with reduces hepatic MGAT and LPGAT activities (pH 6.4, 25°C), slight body weight reduction despite normal food consumption throughout most of the experimental treatment period (only 15% reduction on day 2), reduced serum triacylglycerols and cholesterol but increased total cholesterol accumulation in the liver | Mus musculus | shRNA-adenovirus |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Mus musculus | the infection with a short hairpin RNA-adenovirus construct lowers the lysophosphatidylglycerol acyltransferase 1 expression in diabetic (db/db) mice compared to a control virus infection in a dose-dependent manner, causing a marked reduction in serum triacylglycerol and cholesterol levels, and a significant increase in hepatic cholesterol levels | down |
| Mus musculus | hepatic LPGAT1 expression in diabetic (db/db) mice is 2fold higher than in control (db/m), consistent with 1.5fold higher hepatic monoacylglycerol acyltransferase activity | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | significant role in hepatic triacylglycerol synthesis and secretion in diabetic mice (db/db) | Mus musculus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
