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Literature summary for 2.3.1.22 extracted from

  • Stoveken, T.; Kalscheuer, R.; Malkus, U.; Reichelt, R.; Steinbuchel, A.
    The wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase from Acinetobacter sp. strain ADP1: characterization of a novel type of acyltransferase (2005), J. Bacteriol., 187, 1369-1376.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.22

Cloned(Commentary)

Cloned (Comment) Organism
gene atfA, expression of the soluble enzyme in Escherichia coli strain BL21(DE3) Acinetobacter sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
lipid particle mainly Acinetobacter sp.
-
-
membrane small enzyme amout Acinetobacter sp. 16020
-
additional information determination of subcellular distribution, overview Acinetobacter sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 recombinant enzyme, gel filtration, monomeric form Acinetobacter sp.
53000
-
 1 * 53000, recombinant enzyme, SDS-PAGE Acinetobacter sp.
53000
-
 2 * 53000, recombinant enzyme, SDS-PAGE Acinetobacter sp.
94100
-
 recombinant enzyme, gel filtration, dimeric form Acinetobacter sp.

Organism

Organism UniProt Comment Textmining
Acinetobacter sp.
-
 gene atfA
-
Acinetobacter sp. ADP1
-
 gene atfA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant soluble enzyme from Escherichia coli strain BL21(DE3) by cation exchange, hydrophobic interaction, and anion exchange chromatography, 18.7fold Acinetobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.118
-
 purified recombinant enzyme, substrates palmitoyl-CoA and 1,3-dipalmitoylglycerol Acinetobacter sp.
0.211
-
 purified recombinant enzyme, substrates palmitoyl-CoA and 1,2-dipalmitoylglycerol Acinetobacter sp.

Storage Stability

Storage Stability Organism
-70°C, purified recombinant enzyme, no significant loss after several months Acinetobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the bifunctional enzyme catalyzes the reaction of EC 2.3.1.22, acyl coenzyme A:diacylglycerol acyltransferase, and of EC 2.3.1.75, wax ester synthase, overview, linear acyl acceptor specificity, C14-C18 acceptor substrates in the wax ester synthase reaction are preferred, overview Acinetobacter sp. ?
-
 ?
additional information the bifunctional enzyme catalyzes the reaction of EC 2.3.1.22, acyl coenzyme A:diacylglycerol acyltransferase, and of EC 2.3.1.75, wax ester synthase, overview, linear acyl acceptor specificity, C14-C18 acceptor substrates in the wax ester synthase reaction are preferred, overview Acinetobacter sp. ADP1 ?
-
 ?
palmitoyl-CoA + 1,2-dipalmitoylglycerol i.e. 1,2-dipalmitin Acinetobacter sp. CoA + tripalmitoylglycerol
-
 ?
palmitoyl-CoA + 1,2-dipalmitoylglycerol i.e. 1,2-dipalmitin Acinetobacter sp. ADP1 CoA + tripalmitoylglycerol
-
 ?
palmitoyl-CoA + 1,3-dipalmitoylglycerol i.e. 1,2-dipalmitin Acinetobacter sp. CoA + tripalmitoylglycerol
-
 ?
palmitoyl-CoA + 1,3-dipalmitoylglycerol i.e. 1,2-dipalmitin Acinetobacter sp. ADP1 CoA + tripalmitoylglycerol
-
 ?
palmitoyl-CoA + 2,3-dipalmitoylglycerol i.e. 1,2-dipalmitin Acinetobacter sp. CoA + tripalmitoylglycerol
-
 ?
palmitoyl-CoA + 2,3-dipalmitoylglycerol i.e. 1,2-dipalmitin Acinetobacter sp. ADP1 CoA + tripalmitoylglycerol
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 53000, recombinant enzyme, SDS-PAGE Acinetobacter sp.
monomer 1 * 53000, recombinant enzyme, SDS-PAGE Acinetobacter sp.

Synonyms

Synonyms Comment Organism
acyl coenzyme A:diacylglycerol acyltransferase
-
 Acinetobacter sp.
More cf. EC 2.3.1.75 Acinetobacter sp.
wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase
-
 Acinetobacter sp.
WS/DGAT
-
 Acinetobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 assay at Acinetobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 in phosphate buffer Acinetobacter sp.