BRENDA - Enzyme Database
show all sequences of 2.3.1.219

Curcuminoid biosynthesis by two type III polyketide synthases in the herb Curcuma longa

Katsuyama, Y.; Kita, T.; Funa, N.; Horinouchi, S.; J. Biol. Chem. 284, 11160-11170 (2009)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
DNA and amino acid sequence determination and analysis, expression analysis by quantitative real-time PCR, recombinant expression as N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
Curcuma longa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic analysis of CURS, overview
Curcuma longa
0.085
-
4-coumaroyl-CoA
pH 8.0, 37C, with cinnamoyl-diketide-N-acetylcysteaminyl-CoA
Curcuma longa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(4-coumaroyl)acetyl-CoA + 4-coumaroyl-CoA + H2O
Curcuma longa
-
bisdemethoxycurcumin + CO2 + CoA
-
-
?
(4-coumaroyl)acetyl-CoA + feruloyl-CoA + H2O
Curcuma longa
-
demethoxycurcumin + CO2 + CoA
-
-
?
4-coumaroyl-CoA + feruloylacetyl-CoA
Curcuma longa
-
demethoxycurcumin + CO2 + CoA
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Curcuma longa
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Curcuma longa
-
rhizome
-
Curcuma longa
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(4-coumaroyl)acetyl-CoA + 4-coumaroyl-CoA + H2O
-
719850
Curcuma longa
bisdemethoxycurcumin + CO2 + CoA
-
-
-
?
(4-coumaroyl)acetyl-CoA + feruloyl-CoA + H2O
-
719850
Curcuma longa
demethoxycurcumin + CO2 + CoA
-
-
-
?
4-coumaroyl-CoA + cinnamoyl-diketide-N-acetylcysteamine + H2O
-
719850
Curcuma longa
CoA + cinnamoyl-4-coumaroylmethane + N-acetylcysteamine
-
-
-
?
4-coumaroyl-CoA + feruloylacetyl-CoA
-
719850
Curcuma longa
demethoxycurcumin + CO2 + CoA
-
-
-
?
cinnamoyl-CoA + cinnamoyl-diketide-N-acetylcysteamine + H2O
low activity
719850
Curcuma longa
CoA + dicinnamoylmethane + N-acetylcysteamine
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
curcumin synthase
-
Curcuma longa
CURS
-
Curcuma longa
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Curcuma longa
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.15
-
4-coumaroyl-CoA
pH 8.0, 37C, with cinnamoyl-diketide-N-acetylcysteaminyl-CoA
Curcuma longa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Curcuma longa
Cloned(Commentary) (protein specific)
Commentary
Organism
DNA and amino acid sequence determination and analysis, expression analysis by quantitative real-time PCR, recombinant expression as N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
Curcuma longa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic analysis of CURS, overview
Curcuma longa
0.085
-
4-coumaroyl-CoA
pH 8.0, 37C, with cinnamoyl-diketide-N-acetylcysteaminyl-CoA
Curcuma longa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(4-coumaroyl)acetyl-CoA + 4-coumaroyl-CoA + H2O
Curcuma longa
-
bisdemethoxycurcumin + CO2 + CoA
-
-
?
(4-coumaroyl)acetyl-CoA + feruloyl-CoA + H2O
Curcuma longa
-
demethoxycurcumin + CO2 + CoA
-
-
?
4-coumaroyl-CoA + feruloylacetyl-CoA
Curcuma longa
-
demethoxycurcumin + CO2 + CoA
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Curcuma longa
-
rhizome
-
Curcuma longa
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(4-coumaroyl)acetyl-CoA + 4-coumaroyl-CoA + H2O
-
719850
Curcuma longa
bisdemethoxycurcumin + CO2 + CoA
-
-
-
?
(4-coumaroyl)acetyl-CoA + feruloyl-CoA + H2O
-
719850
Curcuma longa
demethoxycurcumin + CO2 + CoA
-
-
-
?
4-coumaroyl-CoA + cinnamoyl-diketide-N-acetylcysteamine + H2O
-
719850
Curcuma longa
CoA + cinnamoyl-4-coumaroylmethane + N-acetylcysteamine
-
-
-
?
4-coumaroyl-CoA + feruloylacetyl-CoA
-
719850
Curcuma longa
demethoxycurcumin + CO2 + CoA
-
-
-
?
cinnamoyl-CoA + cinnamoyl-diketide-N-acetylcysteamine + H2O
low activity
719850
Curcuma longa
CoA + dicinnamoylmethane + N-acetylcysteamine
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Curcuma longa
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.15
-
4-coumaroyl-CoA
pH 8.0, 37C, with cinnamoyl-diketide-N-acetylcysteaminyl-CoA
Curcuma longa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Curcuma longa
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to the Type III polyketide synthase family
Curcuma longa
metabolism
the enzyme catalyzes a step in the curcuminoid biosynthesis, pathway overview
Curcuma longa
additional information
CURS can collaborate with curcumin synthase, DCS, EC 2.3.1.218, which provides the substrate feruloylacetyl-CoA from condensation of feruloyl-CoA and malonyl-CoA, overview
Curcuma longa
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to the Type III polyketide synthase family
Curcuma longa
metabolism
the enzyme catalyzes a step in the curcuminoid biosynthesis, pathway overview
Curcuma longa
additional information
CURS can collaborate with curcumin synthase, DCS, EC 2.3.1.218, which provides the substrate feruloylacetyl-CoA from condensation of feruloyl-CoA and malonyl-CoA, overview
Curcuma longa
Other publictions for EC 2.3.1.219
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
719493
Katsuyama
Identification and characteriz ...
Curcuma longa
FEBS Lett.
583
2799-2803
2009
-
-
1
-
-
-
-
3
-
-
-
6
-
4
-
-
-
2
-
2
-
-
9
-
4
1
-
-
3
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
3
-
-
-
6
-
-
-
-
-
6
-
-
9
-
3
-
-
3
3
-
-
-
-
1
3
-
3
3
719850
Katsuyama
Curcuminoid biosynthesis by tw ...
Curcuma longa
J. Biol. Chem.
284
11160-11170
2009
-
-
1
-
-
-
-
2
-
-
-
3
-
1
-
-
-
-
-
2
-
-
5
-
2
1
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
3
-
-
-
-
-
2
-
-
5
-
1
-
-
1
1
-
-
-
-
3
3
-
-
-