BRENDA - Enzyme Database
show all sequences of 2.3.1.219

Identification and characterization of multiple curcumin synthases from the herb Curcuma longa

Katsuyama, Y.; Kita, T.; Horinouchi, S.; FEBS Lett. 583, 2799-2803 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
CURS1, DNA and amino acid sequence determination and analysis, expression analysis by quantitative real time PCR; CURS2, DNA and amino acid sequence determination and analysis, expression analysis by quantitative real time PCR; CURS3, DNA and amino acid sequence determination and analysis, expression analysis by quantitative real time PCR
Curcuma longa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0034
-
4-Coumaroyl-CoA
pH 7.5, 37°C, CURS2
Curcuma longa
0.089
-
4-Coumaroyl-CoA
pH 7.5, 37°C, CURS2
Curcuma longa
0.189
-
4-Coumaroyl-CoA
pH 7.5, 37°C, CURS2
Curcuma longa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(4-coumaroyl)acetyl-CoA + 4-coumaroyl-CoA + H2O
Curcuma longa
preferred activity of CURS3
bisdemethoxycurcumin + 2 CO2 + CoA
-
-
?
4-coumaroyl-CoA + feruloylacetyl-CoA
Curcuma longa
preferred activity of CURS3
demethoxycurcumin + CO2 + CoA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Curcuma longa
C0SVZ6
CURS1
-
Curcuma longa
C6L7V8
CURS2
-
Curcuma longa
C6L7V9
CURS3
-
Curcuma longa
-
-
-
Reaction
Reaction
Commentary
Organism
4-coumaroyl-CoA + (4-coumaroyl)acetyl-CoA + H2O = 2 CoA + bisdemethoxycurcumin + CO2
(2)
Curcuma longa
4-coumaroyl-CoA + feruloylacetyl-CoA + H2O = 2 CoA + demethoxycurcumin + CO2
(1)
Curcuma longa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Curcuma longa
-
rhizome
-
Curcuma longa
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(4-coumaroyl)acetyl-CoA + 4-coumaroyl-CoA + H2O
preferred activity of CURS3
719493
Curcuma longa
bisdemethoxycurcumin + 2 CO2 + CoA
-
-
-
?
4-coumaroyl-CoA + feruloylacetyl-CoA
preferred activity of CURS3
719493
Curcuma longa
demethoxycurcumin + CO2 + CoA
-
-
-
?
additional information
substrate specificity, overview. CURS2 prefers feruloyl-CoA as a starter substrate, cf. EC 2.3.1.217, while CURS3 is equally active with both feruloyl-CoA and 4-coumaroyl-CoA. Thus, CURS2 synthesizes curcumin or demethoxycurcumin and CURS3 synthesizes curcumin, bisdemethoxycurcumin and demethoxycurcumin
719493
Curcuma longa
?
-
-
-
-
additional information
substrate specificity, overview. CURS2 prefers feruloyl-CoA as a starter substrate, while CURS3 is equally active with both feruloyl-CoA and 4-coumaroyl-CoA, cf. EC 2.3.1.217. Thus, CURS2 synthesizes curcumin or demethoxycurcumin and CURS3 synthesizes curcumin, bisdemethoxycurcumin and demethoxycurcumin
719493
Curcuma longa
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at; assay at; assay at
Curcuma longa
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.006
-
4-Coumaroyl-CoA
pH 7.5, 37°C, CURS2
Curcuma longa
0.0142
-
4-Coumaroyl-CoA
pH 7.5, 37°C, CURS2
Curcuma longa
0.0157
-
4-Coumaroyl-CoA
pH 7.5, 37°C, CURS2
Curcuma longa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at; assay at; assay at
Curcuma longa
Cloned(Commentary) (protein specific)
Commentary
Organism
CURS1, DNA and amino acid sequence determination and analysis, expression analysis by quantitative real time PCR
Curcuma longa
CURS2, DNA and amino acid sequence determination and analysis, expression analysis by quantitative real time PCR
Curcuma longa
CURS3, DNA and amino acid sequence determination and analysis, expression analysis by quantitative real time PCR
Curcuma longa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0034
-
4-Coumaroyl-CoA
pH 7.5, 37°C, CURS2
Curcuma longa
0.089
-
4-Coumaroyl-CoA
pH 7.5, 37°C, CURS2
Curcuma longa
0.189
-
4-Coumaroyl-CoA
pH 7.5, 37°C, CURS2
Curcuma longa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(4-coumaroyl)acetyl-CoA + 4-coumaroyl-CoA + H2O
Curcuma longa
preferred activity of CURS3
bisdemethoxycurcumin + 2 CO2 + CoA
-
-
?
4-coumaroyl-CoA + feruloylacetyl-CoA
Curcuma longa
preferred activity of CURS3
demethoxycurcumin + CO2 + CoA
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Curcuma longa
-
rhizome
-
Curcuma longa
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(4-coumaroyl)acetyl-CoA + 4-coumaroyl-CoA + H2O
preferred activity of CURS3
719493
Curcuma longa
bisdemethoxycurcumin + 2 CO2 + CoA
-
-
-
?
4-coumaroyl-CoA + feruloylacetyl-CoA
preferred activity of CURS3
719493
Curcuma longa
demethoxycurcumin + CO2 + CoA
-
-
-
?
additional information
substrate specificity, overview. CURS2 prefers feruloyl-CoA as a starter substrate, cf. EC 2.3.1.217, while CURS3 is equally active with both feruloyl-CoA and 4-coumaroyl-CoA. Thus, CURS2 synthesizes curcumin or demethoxycurcumin and CURS3 synthesizes curcumin, bisdemethoxycurcumin and demethoxycurcumin
719493
Curcuma longa
?
-
-
-
-
additional information
substrate specificity, overview. CURS2 prefers feruloyl-CoA as a starter substrate, while CURS3 is equally active with both feruloyl-CoA and 4-coumaroyl-CoA, cf. EC 2.3.1.217. Thus, CURS2 synthesizes curcumin or demethoxycurcumin and CURS3 synthesizes curcumin, bisdemethoxycurcumin and demethoxycurcumin
719493
Curcuma longa
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Curcuma longa
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.006
-
4-Coumaroyl-CoA
pH 7.5, 37°C, CURS2
Curcuma longa
0.0142
-
4-Coumaroyl-CoA
pH 7.5, 37°C, CURS2
Curcuma longa
0.0157
-
4-Coumaroyl-CoA
pH 7.5, 37°C, CURS2
Curcuma longa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Curcuma longa
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to the Type III polyketide synthase family. A Cys-His-Asn catalytic triad is conserved in all known type III PKSs; the enzyme belongs to the Type III polyketide synthase family. A Cys-His-Asn catalytic triad is conserved in all known type III PKSs; the enzyme belongs to the Type III polyketide synthase family. A Cys-His-Asn catalytic triad is conserved in all known type III PKSs
Curcuma longa
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to the Type III polyketide synthase family. A Cys-His-Asn catalytic triad is conserved in all known type III PKSs
Curcuma longa
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.075
-
4-Coumaroyl-CoA
pH 7.5, 37°C, CURS2
Curcuma longa
0.176
-
4-Coumaroyl-CoA
pH 7.5, 37°C, CURS2
Curcuma longa
1.742
-
4-Coumaroyl-CoA
pH 7.5, 37°C, CURS2
Curcuma longa
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.075
-
4-Coumaroyl-CoA
pH 7.5, 37°C, CURS2
Curcuma longa
0.176
-
4-Coumaroyl-CoA
pH 7.5, 37°C, CURS2
Curcuma longa
1.742
-
4-Coumaroyl-CoA
pH 7.5, 37°C, CURS2
Curcuma longa
Other publictions for EC 2.3.1.219
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
719493
Katsuyama
Identification and characteriz ...
Curcuma longa
FEBS Lett.
583
2799-2803
2009
-
-
1
-
-
-
-
3
-
-
-
6
-
4
-
-
-
2
-
2
-
-
9
-
1
-
-
3
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
3
-
-
-
6
-
-
-
-
-
6
-
-
9
-
3
-
-
3
3
-
-
-
-
1
3
-
3
3
719850
Katsuyama
Curcuminoid biosynthesis by tw ...
Curcuma longa
J. Biol. Chem.
284
11160-11170
2009
-
-
1
-
-
-
-
2
-
-
-
3
-
1
-
-
-
-
-
2
-
-
5
-
1
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
3
-
-
-
-
-
2
-
-
5
-
1
-
-
1
1
-
-
-
-
3
3
-
-
-