Cloned (Comment) | Organism |
---|---|
CURS1, DNA and amino acid sequence determination and analysis, expression analysis by quantitative real time PCR | Curcuma longa |
CURS2, DNA and amino acid sequence determination and analysis, expression analysis by quantitative real time PCR | Curcuma longa |
CURS3, DNA and amino acid sequence determination and analysis, expression analysis by quantitative real time PCR | Curcuma longa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0034 | - |
4-coumaroyl-CoA | pH 7.5, 37°C, CURS2 | Curcuma longa | |
0.089 | - |
4-coumaroyl-CoA | pH 7.5, 37°C, CURS2 | Curcuma longa | |
0.189 | - |
4-coumaroyl-CoA | pH 7.5, 37°C, CURS2 | Curcuma longa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4-coumaroyl)acetyl-CoA + 4-coumaroyl-CoA + H2O | Curcuma longa | preferred activity of CURS3 | bisdemethoxycurcumin + 2 CO2 + CoA | - |
? | |
4-coumaroyl-CoA + feruloylacetyl-CoA | Curcuma longa | preferred activity of CURS3 | demethoxycurcumin + CO2 + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Curcuma longa | - |
- |
- |
Curcuma longa | C0SVZ6 | CURS1 | - |
Curcuma longa | C6L7V8 | CURS2 | - |
Curcuma longa | C6L7V9 | CURS3 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4-coumaroyl-CoA + (4-coumaroyl)acetyl-CoA + H2O = 2 CoA + bisdemethoxycurcumin + CO2 | (2) | Curcuma longa | |
4-coumaroyl-CoA + feruloylacetyl-CoA + H2O = 2 CoA + demethoxycurcumin + CO2 | (1) | Curcuma longa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Curcuma longa | - |
rhizome | - |
Curcuma longa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4-coumaroyl)acetyl-CoA + 4-coumaroyl-CoA + H2O | preferred activity of CURS3 | Curcuma longa | bisdemethoxycurcumin + 2 CO2 + CoA | - |
? | |
4-coumaroyl-CoA + feruloylacetyl-CoA | preferred activity of CURS3 | Curcuma longa | demethoxycurcumin + CO2 + CoA | - |
? | |
additional information | substrate specificity, overview. CURS2 prefers feruloyl-CoA as a starter substrate, cf. EC 2.3.1.217, while CURS3 is equally active with both feruloyl-CoA and 4-coumaroyl-CoA. Thus, CURS2 synthesizes curcumin or demethoxycurcumin and CURS3 synthesizes curcumin, bisdemethoxycurcumin and demethoxycurcumin | Curcuma longa | ? | - |
? | |
additional information | substrate specificity, overview. CURS2 prefers feruloyl-CoA as a starter substrate, while CURS3 is equally active with both feruloyl-CoA and 4-coumaroyl-CoA, cf. EC 2.3.1.217. Thus, CURS2 synthesizes curcumin or demethoxycurcumin and CURS3 synthesizes curcumin, bisdemethoxycurcumin and demethoxycurcumin | Curcuma longa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CURS1 | - |
Curcuma longa |
CURS2 | - |
Curcuma longa |
CURS3 | - |
Curcuma longa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Curcuma longa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
4-coumaroyl-CoA | pH 7.5, 37°C, CURS2 | Curcuma longa | |
0.0142 | - |
4-coumaroyl-CoA | pH 7.5, 37°C, CURS2 | Curcuma longa | |
0.0157 | - |
4-coumaroyl-CoA | pH 7.5, 37°C, CURS2 | Curcuma longa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Curcuma longa |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the Type III polyketide synthase family. A Cys-His-Asn catalytic triad is conserved in all known type III PKSs | Curcuma longa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.075 | - |
4-coumaroyl-CoA | pH 7.5, 37°C, CURS2 | Curcuma longa | |
0.176 | - |
4-coumaroyl-CoA | pH 7.5, 37°C, CURS2 | Curcuma longa | |
1.742 | - |
4-coumaroyl-CoA | pH 7.5, 37°C, CURS2 | Curcuma longa |