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Literature summary for 2.3.1.219 extracted from

  • Katsuyama, Y.; Kita, T.; Horinouchi, S.
    Identification and characterization of multiple curcumin synthases from the herb Curcuma longa (2009), FEBS Lett., 583, 2799-2803.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
CURS1, DNA and amino acid sequence determination and analysis, expression analysis by quantitative real time PCR Curcuma longa
CURS2, DNA and amino acid sequence determination and analysis, expression analysis by quantitative real time PCR Curcuma longa
CURS3, DNA and amino acid sequence determination and analysis, expression analysis by quantitative real time PCR Curcuma longa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0034
-
4-coumaroyl-CoA pH 7.5, 37°C, CURS2 Curcuma longa
0.089
-
4-coumaroyl-CoA pH 7.5, 37°C, CURS2 Curcuma longa
0.189
-
4-coumaroyl-CoA pH 7.5, 37°C, CURS2 Curcuma longa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(4-coumaroyl)acetyl-CoA + 4-coumaroyl-CoA + H2O Curcuma longa preferred activity of CURS3 bisdemethoxycurcumin + 2 CO2 + CoA
-
?
4-coumaroyl-CoA + feruloylacetyl-CoA Curcuma longa preferred activity of CURS3 demethoxycurcumin + CO2 + CoA
-
?

Organism

Organism UniProt Comment Textmining
Curcuma longa
-
-
-
Curcuma longa C0SVZ6 CURS1
-
Curcuma longa C6L7V8 CURS2
-
Curcuma longa C6L7V9 CURS3
-

Reaction

Reaction Comment Organism Reaction ID
4-coumaroyl-CoA + (4-coumaroyl)acetyl-CoA + H2O = 2 CoA + bisdemethoxycurcumin + CO2 (2) Curcuma longa
4-coumaroyl-CoA + feruloylacetyl-CoA + H2O = 2 CoA + demethoxycurcumin + CO2 (1) Curcuma longa

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Curcuma longa
-
rhizome
-
Curcuma longa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(4-coumaroyl)acetyl-CoA + 4-coumaroyl-CoA + H2O preferred activity of CURS3 Curcuma longa bisdemethoxycurcumin + 2 CO2 + CoA
-
?
4-coumaroyl-CoA + feruloylacetyl-CoA preferred activity of CURS3 Curcuma longa demethoxycurcumin + CO2 + CoA
-
?
additional information substrate specificity, overview. CURS2 prefers feruloyl-CoA as a starter substrate, cf. EC 2.3.1.217, while CURS3 is equally active with both feruloyl-CoA and 4-coumaroyl-CoA. Thus, CURS2 synthesizes curcumin or demethoxycurcumin and CURS3 synthesizes curcumin, bisdemethoxycurcumin and demethoxycurcumin Curcuma longa ?
-
?
additional information substrate specificity, overview. CURS2 prefers feruloyl-CoA as a starter substrate, while CURS3 is equally active with both feruloyl-CoA and 4-coumaroyl-CoA, cf. EC 2.3.1.217. Thus, CURS2 synthesizes curcumin or demethoxycurcumin and CURS3 synthesizes curcumin, bisdemethoxycurcumin and demethoxycurcumin Curcuma longa ?
-
?

Synonyms

Synonyms Comment Organism
CURS1
-
Curcuma longa
CURS2
-
Curcuma longa
CURS3
-
Curcuma longa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Curcuma longa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.006
-
4-coumaroyl-CoA pH 7.5, 37°C, CURS2 Curcuma longa
0.0142
-
4-coumaroyl-CoA pH 7.5, 37°C, CURS2 Curcuma longa
0.0157
-
4-coumaroyl-CoA pH 7.5, 37°C, CURS2 Curcuma longa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Curcuma longa

General Information

General Information Comment Organism
evolution the enzyme belongs to the Type III polyketide synthase family. A Cys-His-Asn catalytic triad is conserved in all known type III PKSs Curcuma longa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.075
-
4-coumaroyl-CoA pH 7.5, 37°C, CURS2 Curcuma longa
0.176
-
4-coumaroyl-CoA pH 7.5, 37°C, CURS2 Curcuma longa
1.742
-
4-coumaroyl-CoA pH 7.5, 37°C, CURS2 Curcuma longa