Literature summary for 2.3.1.217 extracted from

Rodrigues, J.L.; Araujo, R.G.; Prather, K.L.; Kluskens, L.D.; Rodrigues, L.R.
Production of curcuminoids from tyrosine by a metabolically engineered Escherichia coli using caffeic acid as an intermediate (2015), Biotechnol. J., 10, 599-609.

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Application

Application	Comment	Organism
synthesis	production of curcuminoids using an engineered artificial pathway in Escherichia coli. Expression of Arabidopsis thaliana 4-coumaroyl-CoA ligase and Curcuma longa diketide-CoA synthase (DCS) and curcumin synthase (CURS1) leads to synthesis of 70 mg/l of curcumin from ferulic acid. Bisdemethoxycurcumin and demethoxycurcumin are produced, but in lower concentrations, by feeding 4-coumaric acid or a mixture of 4-coumaric acid and ferulic acid, respectively. To produce caffeic acid, tyrosine ammonia lyase from Rhodotorula glutinis and 4-coumarate 3-hydroxylase from Saccharothrix espanaensis are used. Caffeoyl-CoA 3-O-methyltransferase from Medicago sativa converts caffeoyl-CoA to feruloyl-CoA. Using caffeic acid, 4-coumaric acid or tyrosine as a substrate, 3.9, 0.3, and 0.2 mg/l of curcumin are produced, respectively	Curcuma longa

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Curcuma longa

Organism

Organism	UniProt	Comment	Textmining
Curcuma longa	C0SVZ6	-	-

Synonyms

Synonyms	Comment	Organism
CURS1	-	Curcuma longa

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Release 2023.1 (January 2023)