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Literature summary for 2.3.1.216 extracted from

  • Morita, H.; Kondo, S.; Abe, T.; Noguchi, H.; Sugio, S.; Abe, I.; Kohno, T.
    Crystallization and preliminary crystallographic analysis of a novel plant type III polyketide synthase that produces pentaketide chromone (2006), Acta Crystallogr. Sect. F, 62, 899-901.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.216

Cloned(Commentary)

Cloned (Comment) Organism
expression of N-terminally GST-tagged pentaketide chromone synthase in Escherichia coli strain BL21(DE3) Aloe arborescens

Crystallization (Commentary)

Crystallization (Comment) Organism
purified detagged recombinant pentaketide chromone synthase, hanging drop vapour diffusion method, mixing of 500 nl of 10 mg/ml protein in 20 mM HEPES-NaOH, pH 7.0, containing 100 mM NaCl, and 2 mM DTT, with 500 nl reservoir solution containing 100 mM Tris-HCl, pH 8.5, 14% w/v PEG 8000, and 350 mM KF, 20°C, 2 days, X-ray diffraction structure determination and analysis at 1.6 A resolution Aloe arborescens

Protein Variants

Protein Variants Comment Organism
M207G site-directed mutagenesis, the mutant enzyme efficiently catalyzes the successive condensation of eight molecules of malonyl-CoA to produce 2,7-dihydroxy-5-[(4-hydroxy-2-oxo-2H-pyran-6-yl)methyl]-2-methyl-2,3-dihydro-4H-chromen-4-one and 2,7-dihydroxy-5-[(4-hydroxy-2-oxo-2H-pyran-6-yl)methyl]-5-methyl-2,3-dihydro-4H-chromen-4-one, i.e. SEK4 and SEK4b. The pentaketide-forming pentaketide chromone synthase is thus functionally transformed into an octaketide-producing enzyme by the single amino-acid substitution Aloe arborescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5 malonyl-CoA Aloe arborescens
-
 5 CoA + 5,7-dihydroxy-2-methyl-4H-chromen-4-one + 5 CO2 + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Aloe arborescens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally GST-tagged pentaketide chromone synthase from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, removal of the GST-tag, anion exchange chromatography, and gel filtration to homogeneity Aloe arborescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5 malonyl-CoA
-
 Aloe arborescens 5 CoA + 5,7-dihydroxy-2-methyl-4H-chromen-4-one + 5 CO2 + H2O
-
 ?

Synonyms

Synonyms Comment Organism
PCS
-
 Aloe arborescens
pentaketide chromone synthase
-
 Aloe arborescens

General Information

General Information Comment Organism
evolution pentaketide chromone synthase is a plant-specific type III polyketide synthase that belongs to the chalcone synthase superfamily of type III polyketide synthases Aloe arborescens
physiological function the plant-specific type III polyketide synthase that produces 5,7-dihydroxy-2-methylchromone from five molecules of malonyl-CoA. The aromatic pentaketide is a biosynthetic precursor of the anti-asthmatic furochromones kehellin and visnagin Aloe arborescens