KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state enzyme kinetics, overview | Rheum palmatum | |
0.01 | - |
4-coumaroyl-CoA | pH 8.0, 30°C, recombinant wild-type enzyme | Rheum palmatum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + malonyl-CoA + H2O | Rheum palmatum | - |
2 CoA + 4-hydroxybenzalacetone + 2 CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rheum palmatum | Q94FV7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + malonyl-CoA + H2O | - |
Rheum palmatum | 2 CoA + 4-hydroxybenzalacetone + 2 CO2 | - |
? | |
4-coumaroyl-CoA + malonyl-CoA + H2O | one-step condensation | Rheum palmatum | 2 CoA + 4-hydroxybenzalacetone + 2 CO2 | - |
? | |
anthraniloyl-CoA + malonyl-CoA + H2O | - |
Rheum palmatum | 4-hydroxy-1,3-dimethyl-2(1H)-quinolone + ? | - |
? | |
anthraniloyl-CoA + methylmalonyl-CoA + H2O | - |
Rheum palmatum | ? | - |
? | |
additional information | the enzyme reaction with the anthraniloyl-CoA proceeds without the decarboxylation step, and the amide formation immediately follows the condensation reactions of N-methylanthraniloyl-CoA (or anthraniloyl-CoA) and malonyl-CoA (or methylmalonyl-CoA), mechanism, overview | Rheum palmatum | ? | - |
? | |
N-methylanthraniloyl-CoA + 3 malonyl-CoA + H2O | - |
Rheum palmatum | 1,3-dihydroxy-N-methylacridone + ? | - |
? | |
N-methylanthraniloyl-CoA + methylmalonyl-CoA + H2O | - |
Rheum palmatum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
bAS | - |
Rheum palmatum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rheum palmatum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0298 | - |
4-coumaroyl-CoA | pH 8.0, 30°C, recombinant wild-type enzyme | Rheum palmatum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Rheum palmatum |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a plant-specific type III polyketide synthase of the chalcone synthase (CHS) superfamily | Rheum palmatum |
physiological function | benzalacetone synthase from Rheum palmatum efficiently catalyzes condensation of N-methylanthraniloyl-CoA (or anthraniloyl-CoA) with malonyl-CoA (or methylmalonyl-CoA) to produce 4-hydroxy-2(1H)-quinolones, a novel alkaloidal scaffold produced by a type III polyketide synthase, PKS. The quinolone alkaloids act as N-methyl-D-aspartate and serotonin 5-HT3 receptor antagonists. Moreover, they are also important intermediates in the chemical synthesis of alkaloids | Rheum palmatum |