Cloned (Comment) | Organism |
---|---|
construction of chimeric L-CPT I with deletions and exchanged C-terminal sequences between rat and pig enzymes, expression in Pichia pastoris | Rattus norvegicus |
construction of chimeric L-CPT I with deletions and exchanged C-terminal sequences between rat and pig enzymes, expression in Pichia pastoris | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
malonyl-CoA | sensitivity to inhibition of deletion and chimeric L-CPT I mutants | Rattus norvegicus | |
malonyl-CoA | sensitivity to inhibition of deletion and chimeric L-CPT I mutants | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km values of deletion and chimeric L-CPT I mutants | Rattus norvegicus | |
additional information | - |
additional information | Km values of deletion and chimeric L-CPT I mutants | Sus scrofa | |
0.035 | - |
palmitoyl-CoA | recombinant wild-type L-CPT I | Sus scrofa | |
0.043 | - |
palmitoyl-CoA | recombinant wild-type L-CPT I | Rattus norvegicus | |
0.1 | - |
L-carnitine | recombinant wild-type L-CPT I | Rattus norvegicus | |
0.126 | - |
L-carnitine | recombinant wild-type L-CPT I | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + L-carnitine | Rattus norvegicus | - |
CoA + L-acylcarnitine | - |
? | |
acyl-CoA + L-carnitine | Sus scrofa | - |
CoA + L-acylcarnitine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Sus scrofa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activities of deletion and chimeric L-CPT I mutants | Rattus norvegicus |
additional information | - |
activities of deletion and chimeric L-CPT I mutants | Sus scrofa |
0.0052 | - |
recombinant wild-type L-CPT I | Sus scrofa |
0.0078 | - |
recombinant wild-type L-CPT I | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + L-carnitine | - |
Rattus norvegicus | CoA + L-acylcarnitine | - |
? | |
acyl-CoA + L-carnitine | - |
Sus scrofa | CoA + L-acylcarnitine | - |
? | |
palmitoyl-CoA + L-carnitine | chimeric L-CPT I with exchanged C-terminals between pig and rat enzymes | Rattus norvegicus | CoA + L-palmitoylcarnitine | - |
? | |
palmitoyl-CoA + L-carnitine | chimeric L-CPT I with exchanged C-terminals between pig and rat enzymes | Sus scrofa | CoA + L-palmitoylcarnitine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rattus norvegicus |
30 | - |
assay at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Rattus norvegicus |
7 | - |
assay at | Sus scrofa |