Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.21 extracted from

  • De Vries, Y.; Arvidson, D.N.; Waterham, H.R.; Cregg, J.M.; Woldegiorgis, G.
    Functional characterization of mitochondrial carnitine palmitoyltransferases I and II expressed in the yeast Pichia pastoris (1997), Biochemistry, 36, 5285-5292.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
clonig of CPT I and II from liver, expression in Pichia pastoris Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
malonyl-CoA CPT I; CPT I catalytic activity and malonyl-CoA sensitivity is located on 1 single polypeptide; CPT II not inhibited; recombinant CPT I is highly sensitive, recombinant CPT II not Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.105
-
L-carnitine recombinant CPT II Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial outer membrane CPT I is located in the outer membrane Rattus norvegicus 5741
-
mitochondrion CPT II Rattus norvegicus 5739
-
mitochondrion CPT I Rattus norvegicus 5739
-
additional information forward reaction by CPT I at outer mitochondrial membrane, transport of acylcarnitine through mitochondrial membrane, reverse reaction at inner face of inner membrane by CPT II, overview Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
x * 70000, recombinant CPT II, SDS-PAGE Rattus norvegicus
88000
-
x * 88000, recombinant CPT I, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + L-carnitine Rattus norvegicus CPT I is the rate-limiting enzyme in beta-oxidation of long-chain fatty acids CoA + L-acylcarnitine
-
?
palmitoyl-CoA + L-carnitine Rattus norvegicus forward reaction by CPT I and reverse reaction by CPT II L-palmitoylcarnitine + CoA
-
r

Organism

Organism UniProt Comment Textmining
no activity in Pichia pastoris
-
-
-
Rattus norvegicus
-
enzyme form CPT I
-
Rattus norvegicus
-
enzyme form CPT II
-

Purification (Commentary)

Purification (Comment) Organism
partial, recombinant CPT I and II from Pichia pastoris Rattus norvegicus

Renatured (Commentary)

Renatured (Comment) Organism
reconstitution of detergent-inactivated recombinant M-CPT I, purified from Pichia pastoris, by removal of detergents in presence of phospholipids Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver CPT II Rattus norvegicus
-
liver CPT I Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0032
-
mitochondrial fraction of recombinant Pichia pastoris expressing CPT I Rattus norvegicus
0.0069
-
mitochondrial fraction of recombinant Pichia pastoris expressing CPT II Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + L-carnitine
-
Rattus norvegicus CoA + L-acylcarnitine
-
?
acyl-CoA + L-carnitine CPT I is the rate-limiting enzyme in beta-oxidation of long-chain fatty acids Rattus norvegicus CoA + L-acylcarnitine
-
?
palmitoyl-CoA + L-carnitine
-
Rattus norvegicus CoA + L-palmitoylcarnitine
-
r
palmitoyl-CoA + L-carnitine forward reaction by CPT I and reverse reaction by CPT II Rattus norvegicus L-palmitoylcarnitine + CoA
-
r

Subunits

Subunits Comment Organism
? x * 88000, recombinant CPT I, SDS-PAGE Rattus norvegicus
? x * 70000, recombinant CPT II, SDS-PAGE Rattus norvegicus