Cloned (Comment) | Organism |
---|---|
clonig of CPT I and II from liver, expression in Pichia pastoris | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
malonyl-CoA | CPT I; CPT I catalytic activity and malonyl-CoA sensitivity is located on 1 single polypeptide; CPT II not inhibited; recombinant CPT I is highly sensitive, recombinant CPT II not | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.105 | - |
L-carnitine | recombinant CPT II | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial outer membrane | CPT I is located in the outer membrane | Rattus norvegicus | 5741 | - |
mitochondrion | CPT II | Rattus norvegicus | 5739 | - |
mitochondrion | CPT I | Rattus norvegicus | 5739 | - |
additional information | forward reaction by CPT I at outer mitochondrial membrane, transport of acylcarnitine through mitochondrial membrane, reverse reaction at inner face of inner membrane by CPT II, overview | Rattus norvegicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
x * 70000, recombinant CPT II, SDS-PAGE | Rattus norvegicus |
88000 | - |
x * 88000, recombinant CPT I, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + L-carnitine | Rattus norvegicus | CPT I is the rate-limiting enzyme in beta-oxidation of long-chain fatty acids | CoA + L-acylcarnitine | - |
? | |
palmitoyl-CoA + L-carnitine | Rattus norvegicus | forward reaction by CPT I and reverse reaction by CPT II | L-palmitoylcarnitine + CoA | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
no activity in Pichia pastoris | - |
- |
- |
Rattus norvegicus | - |
enzyme form CPT I | - |
Rattus norvegicus | - |
enzyme form CPT II | - |
Purification (Comment) | Organism |
---|---|
partial, recombinant CPT I and II from Pichia pastoris | Rattus norvegicus |
Renatured (Comment) | Organism |
---|---|
reconstitution of detergent-inactivated recombinant M-CPT I, purified from Pichia pastoris, by removal of detergents in presence of phospholipids | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | CPT II | Rattus norvegicus | - |
liver | CPT I | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0032 | - |
mitochondrial fraction of recombinant Pichia pastoris expressing CPT I | Rattus norvegicus |
0.0069 | - |
mitochondrial fraction of recombinant Pichia pastoris expressing CPT II | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + L-carnitine | - |
Rattus norvegicus | CoA + L-acylcarnitine | - |
? | |
acyl-CoA + L-carnitine | CPT I is the rate-limiting enzyme in beta-oxidation of long-chain fatty acids | Rattus norvegicus | CoA + L-acylcarnitine | - |
? | |
palmitoyl-CoA + L-carnitine | - |
Rattus norvegicus | CoA + L-palmitoylcarnitine | - |
r | |
palmitoyl-CoA + L-carnitine | forward reaction by CPT I and reverse reaction by CPT II | Rattus norvegicus | L-palmitoylcarnitine + CoA | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 88000, recombinant CPT I, SDS-PAGE | Rattus norvegicus |
? | x * 70000, recombinant CPT II, SDS-PAGE | Rattus norvegicus |