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Literature summary for 2.3.1.209 extracted from
- Biochemical characterization of dTDP-D-Qui4N and dTDP-D-Qui4NAc biosynthetic pathways in Shigella dysenteriae type 7 and Escherichia coli O7 (2007), J. Bacteriol., 189, 8626-8635.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | the enzyme might be a valuable source for the production of dTDP-D-Qui4N and dTDP-D-Qui4NAc, which are potentially useful in the pharmaceutical industry for drug development | Shigella dysenteriae |
| drug development | the enzyme might be a valuable source for the production of dTDP-D-Qui4N and dTDP-D-Qui4NAc, which are potentially useful in the pharmaceutical industry for drug development | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis | Shigella dysenteriae |
| from strain G1112, DNA and amino acid sequence determination and analysis, overexpression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | 96% inhibition at 5 mM | Escherichia coli |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.142 | - |
dTDP-4-amino-4,6-dideoxy-alpha-D-glucose | pH 7.4, 37°C, recombinant His-tagged enzyme | Escherichia coli | |
| 0.554 | - |
acetyl-CoA | pH 7.4, 37°C, recombinant His-tagged enzyme | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no effect by presence of Mg2+, Ca2+, Mn2+, and Co2+or EDTA on enzyne activity, the enzyme is divalent cation-independent | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27100 | - |
x * 27100, recombinant His-tagged enzyme, SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-glucose | Shigella dysenteriae | - |
CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-glucose | - |
? | |
| acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-glucose | Escherichia coli | - |
CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-glucose | - |
? | |
| acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-glucose | Escherichia coli O7 | - |
CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | Q9XCW3 | O7 type strain | - |
| Escherichia coli O7 | Q9XCW3 | O7 type strain | - |
| Shigella dysenteriae | - |
serotype 7 type strain, gene vioB | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-glucose | - |
Shigella dysenteriae | CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-glucose | - |
? | |
| acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-glucose | - |
Escherichia coli | CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-glucose | - |
? | |
| acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-glucose | - |
Escherichia coli | CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-glucose | product identificatin by capillary electrophoresis, electrospray, ionization-mass spectrometry, and NMR spectroscopy, overview | ? | |
| acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-glucose | - |
Shigella dysenteriae | CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-glucose | product identification by capillary electrophoresis, electrospray, ionization-mass spectrometry, and NMR spectroscopy, overview | ? | |
| acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-glucose | - |
Escherichia coli O7 | CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-glucose | - |
? | |
| acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-glucose | - |
Escherichia coli O7 | CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-glucose | product identificatin by capillary electrophoresis, electrospray, ionization-mass spectrometry, and NMR spectroscopy, overview | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 27100, recombinant His-tagged enzyme, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| VioB | - |
Shigella dysenteriae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Shigella dysenteriae |
| 37 | - |
assay at | Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 65 | activity range, complete substrate conversion at 25-50°C | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 21.6 | - |
acetyl-CoA | pH 7.4, 37°C, recombinant His-tagged enzyme | Escherichia coli | |
| 155.3 | - |
dTDP-4-amino-4,6-dideoxy-alpha-D-glucose | pH 7.4, 37°C, recombinant His-tagged enzyme | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Shigella dysenteriae |
| 7.4 | - |
assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | - |
Shigella dysenteriae | |
| acetyl-CoA | - |
Escherichia coli | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | dTDP-D-Qui4N is synthesized from alpha-D-glucose-1-phosphate in three reaction steps catalyzed by glucose-1-phosphate thymidyltransferase (RmlA), dTDP-D-glucose 4,6-dehydratase (RmlB), and dTDP-4-keto-6-deoxy-D-glucose aminotransferase (VioA). An additional acetyltransferase (VioB) catalyzes the conversion of dTDP-D-Qui4N into dTDP-D-Qui4NAc, biosynthetic pathway, overview | Shigella dysenteriae |
| metabolism | dTDP-D-Qui4N is synthesized from alpha-D-glucose-1-phosphate in three reaction steps catalyzed by glucose-1-phosphate thymidyltransferase (RmlA), dTDP-D-glucose 4,6-dehydratase (RmlB), and dTDP-4-keto-6-deoxy-D-glucose aminotransferase (VioA). An additional acetyltransferase (VioB) catalyzes the conversion of dTDP-D-Qui4N into dTDP-D-Qui4NAc, biosynthetic pathway, overview | Escherichia coli |
| physiological function | the O antigens of Escherichia coli O7 contains 4-acetamido-4,6-dideoxy-D-glucose, i.e. D-Qui4NAc | Escherichia coli |
| physiological function | the O antigens of Shigella dysenteriae type 7 contains 4-(N-acetylglycyl)amino-4,6-dideoxy-D-glucose, i.e. D-Qui4NGlyAc | Shigella dysenteriae |
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