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Literature summary for 2.3.1.201 extracted from
- Biosynthesis of UDP-GlcNAc(3NAc)A by WbpB, WbpE, and WbpD: enzymes in the Wbp pathway responsible for O-antigen assembly in Pseudomonas aeruginosa PAO1 (2009), Biochemistry, 48, 5446-5455.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21-CodonPlus(DE3) RIL cells | Pseudomonas aeruginosa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.107 | - |
UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate | in 50 mM HEPES (pH 8.0), 2.5 mM dithiothreitol, and 2 mM MgCl2, at 30°C | Pseudomonas aeruginosa |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
serotype O5 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose resin column chromatography, gel filtration | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate | - |
Pseudomonas aeruginosa | CoA + UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate | - |
? | |
| additional information | no activity with UDP-GlcNAcA, UDP-GlcNAc, and UDP-2-acetamido-4-amino-2,4,6-trideoxy-D-glucosamine | Pseudomonas aeruginosa | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDP-2-acetamido-3-amino-2,3-dideoxy-glucuronate N-acetyltransferase | - |
Pseudomonas aeruginosa |
| WbpD | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Pseudomonas aeruginosa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 65 | - |
Pseudomonas aeruginosa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 48.3 | - |
UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate | in 50 mM HEPES (pH 8.0), 2.5 mM dithiothreitol, and 2 mM MgCl2, at 30°C | Pseudomonas aeruginosa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Pseudomonas aeruginosa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 10 | - |
Pseudomonas aeruginosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Pseudomonas aeruginosa | |
| pyridoxal 5'-phosphate | - |
Pseudomonas aeruginosa | |
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