Cloned (Comment) | Organism |
---|---|
expressed as an N-terminally histidine-tagged fusion protein in Escherichia coli BL21(DE3) pLysS cells | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
K136A | the mutation reduces the stabilizing effects of acetyl-CoA | Pseudomonas aeruginosa |
K136R | the mutation showed no discernible effect the stabilizing effects of acetyl-CoA on the purified mutant protein | Pseudomonas aeruginosa |
General Stability | Organism |
---|---|
incubation of the enzyme with acetyl-CoA significantly enhances the stability of the protein and prevents precipitation over a course of 14 days | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22726 | - |
3 * 22726, His-tagged enzyme, calculated from amino acid sequence | Pseudomonas aeruginosa |
22726 | - |
3 * 22726, MALDI-TOF mass spectrometry | Pseudomonas aeruginosa |
23000 | - |
3 * 23000, His-tagged enzyme, SDS-PAGE | Pseudomonas aeruginosa |
61000 | - |
His-tagged enzyme, gel filtration | Pseudomonas aeruginosa |
68180 | - |
MALDI-TOF mass spectrometry | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
serotype O5 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate | - |
Pseudomonas aeruginosa | CoA + UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 3 * 22726, His-tagged enzyme, calculated from amino acid sequence | Pseudomonas aeruginosa |
homotrimer | 3 * 22726, MALDI-TOF mass spectrometry | Pseudomonas aeruginosa |
homotrimer | 3 * 23000, His-tagged enzyme, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid3-N-acetyltransferase | - |
Pseudomonas aeruginosa |
WbpD | - |
Pseudomonas aeruginosa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas aeruginosa | pI-value of above 8.0, isoelectric focusing | - |
8 |
Pseudomonas aeruginosa | calculated from amino acid sequence | - |
8.2 |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is required for B-band O-antigen biosynthesis in Pseudomonas aeruginosa | Pseudomonas aeruginosa |