Cloned (Comment) | Organism |
---|---|
gene Rv3371, specific to pathogenic mycobacteria, leads to modifications in colony morphotype, bacterial architecture, cell surface properties, and to elevated triacylglycerol (TAG) levels when expressed in Mycolicibacterium smegmatis (Mycobacterium smegmatis) | Mycobacterium tuberculosis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell membrane | mainly | Mycobacterium tuberculosis | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1,2-diacyl-sn-glycerol | Mycobacterium tuberculosis | - |
CoA + 1,2,3-triacylglycerol | - |
? | |
acyl-CoA + 1,2-diacyl-sn-glycerol | Mycobacterium tuberculosis H37Rv | - |
CoA + 1,2,3-triacylglycerol | - |
? | |
acyl-CoA + 1,2-diacyl-sn-glycerol | Mycobacterium tuberculosis ATCC 25618 | - |
CoA + 1,2,3-triacylglycerol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WKA9 | - |
- |
Mycobacterium tuberculosis ATCC 25618 | P9WKA9 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WKA9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1,2-diacyl-sn-glycerol | - |
Mycobacterium tuberculosis | CoA + 1,2,3-triacylglycerol | - |
? | |
acyl-CoA + 1,2-diacyl-sn-glycerol | - |
Mycobacterium tuberculosis H37Rv | CoA + 1,2,3-triacylglycerol | - |
? | |
acyl-CoA + 1,2-diacyl-sn-glycerol | - |
Mycobacterium tuberculosis ATCC 25618 | CoA + 1,2,3-triacylglycerol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DGAT | - |
Mycobacterium tuberculosis |
diacylglycerol acyltransferase | - |
Mycobacterium tuberculosis |
Rv3371 | - |
Mycobacterium tuberculosis |
Organism | Comment | Expression |
---|---|---|
Mycobacterium tuberculosis | the Rv3371 promoter is minimally active during exponential growth in vitro, but is upregulated under stationary phase, hypoxia, nutrient starvation, nitrosative stress, low iron, in IFN-gamma activated macrophages and infected mice. The low iron-induced expression of Rv3371 is likely due to the de-repression by Rv1404, which is probably activated by ideR | up |
General Information | Comment | Organism |
---|---|---|
malfunction | a Rv3371 deletion mutant of Mtb shows impaired non-replicating persistence in vitro and altered sensitivity to anti-mycobacterial drugs. In low iron medium, the Rv3371 deletion mutant shows reduced formation of TAG containing extracellular vesicles | Mycobacterium tuberculosis |
physiological function | the diacylglycerol acyltransferase Rv3371 of Mycobacterium tuberculosis is required for growth arrest and involved in stress-induced cell wall alterations during persistence. It is involved in the biosynthesis of triacylglycerol (TAG). TAG is important to mycobacteria both as cell envelope component and energy reservoir | Mycobacterium tuberculosis |