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Literature summary for 2.3.1.20 extracted from

  • Haili, N.; Louap, J.; Canonge, M.; Jagic, F.; Louis-Mondesir, C.; Chardot, T.; Briozzo, P.
    Expression of soluble forms of yeast diacylglycerol acyltransferase 2 that integrate a broad range of saturated fatty acids in triacylglycerols (2016), PLoS ONE, 11, e0165431 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.20

Cloned(Commentary)

Cloned (Comment) Organism
gene YALI0_E32769g, sequence comparisons, recombinant expression of MBP-tagged wild-type enzyme and mutants in Escherichia coli strain BL21(DE3) pLysS Yarrowia lipolytica

Protein Variants

Protein Variants Comment Organism
additional information construction of enzyme mutants Dga1pDELTA19, lacking a region predicted to be disordered, and Dga1pDELTA85, lacking the 85 N-terminal residues predicted to contain a transmembrane domain. Mutant Dga1pDELTA19 shows reduced activity compared to wild-type, while mutant Dga1pDELTA85 is inactive Yarrowia lipolytica

Inhibitors

Inhibitors Comment Organism Structure
niacin a specific noncompetitive DGAT2 inhibitor. Dga1p enzyme mutant versions exhibit DGAT2 specific activity, which is inhibited by niacin. The decrease is 70% for Dga1pDELTA19 and up to 99.5% for Dga1pDELTA85 Yarrowia lipolytica

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane the enzyme contains three putative transmembrane domains Yarrowia lipolytica 16020
-
additional information topology prediction, indicating three putative transmembrane domains, hydrophobicity values Yarrowia lipolytica
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Yarrowia lipolytica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + 1,2-diacyl-sn-glycerol Yarrowia lipolytica
-
 CoA + 1,2,3-triacylglycerol
-
 ?

Organism

Organism UniProt Comment Textmining
Yarrowia lipolytica Q6C3R2 i.e. Candida lipolytica
-
Yarrowia lipolytica CLIB 122 Q6C3R2 i.e. Candida lipolytica
-
Yarrowia lipolytica E 150 Q6C3R2 i.e. Candida lipolytica
-

Purification (Commentary)

Purification (Comment) Organism
recombinant MBP-tagged wild-type enzyme and mutants from Escherichia coli strain BL21(DE3) pLysS by amylose affinity chromatography and gel filtration Yarrowia lipolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Yarrowia lipolytica CoA + 1,2,3-triacylglycerol
-
 ?
lauroyl-CoA + 1,2-dioleoyl-sn-glycerol
-
 Yarrowia lipolytica CoA + 1,2-dioleoyl-3-lauroyl-sn-glycerol
-
 ?
linoleoyl-CoA + 1,2-dioleoyl-sn-glycerol
-
 Yarrowia lipolytica CoA + 1,2-dioleoyl-3-linoleoyl-sn-glycerol
-
 ?
linoleoyl-CoA + 1,2-dioleoyl-sn-glycerol
-
 Yarrowia lipolytica CLIB 122 CoA + 1,2-dioleoyl-3-linoleoyl-sn-glycerol
-
 ?
linoleoyl-CoA + 1,2-dioleoyl-sn-glycerol
-
 Yarrowia lipolytica E 150 CoA + 1,2-dioleoyl-3-linoleoyl-sn-glycerol
-
 ?
additional information acyl-CoA substrate specificity analysis of wild-type and mutant enzymes, overview. Dga1p enzymes can integrate saturated substrates of different length. Dga1p prefers long chain saturated acyl-CoAs as acyl donors in vitro. Also mutant Dga1pDELTA85 is less efficiently with the C12:0-CoA substrate than with longer substrates Yarrowia lipolytica ?
-
-
additional information acyl-CoA substrate specificity analysis of wild-type and mutant enzymes, overview. Dga1p enzymes can integrate saturated substrates of different length. Dga1p prefers long chain saturated acyl-CoAs as acyl donors in vitro. Also mutant Dga1pDELTA85 is less efficiently with the C12:0-CoA substrate than with longer substrates Yarrowia lipolytica CLIB 122 ?
-
-
additional information acyl-CoA substrate specificity analysis of wild-type and mutant enzymes, overview. Dga1p enzymes can integrate saturated substrates of different length. Dga1p prefers long chain saturated acyl-CoAs as acyl donors in vitro. Also mutant Dga1pDELTA85 is less efficiently with the C12:0-CoA substrate than with longer substrates Yarrowia lipolytica E 150 ?
-
-
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
-
 Yarrowia lipolytica CoA + 1,2,3-trioleoyl-sn-glycerol
-
 ?
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
-
 Yarrowia lipolytica CLIB 122 CoA + 1,2,3-trioleoyl-sn-glycerol
-
 ?
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
-
 Yarrowia lipolytica E 150 CoA + 1,2,3-trioleoyl-sn-glycerol
-
 ?
palmitoyl-CoA + 1,2-dioleoyl-sn-glycerol
-
 Yarrowia lipolytica CoA + 1,2-dioleoyl-3-palmitoyl-sn-glycerol
-
 ?
palmitoyl-CoA + 1,2-dioleoyl-sn-glycerol
-
 Yarrowia lipolytica CLIB 122 CoA + 1,2-dioleoyl-3-palmitoyl-sn-glycerol
-
 ?
palmitoyl-CoA + 1,2-dioleoyl-sn-glycerol
-
 Yarrowia lipolytica E 150 CoA + 1,2-dioleoyl-3-palmitoyl-sn-glycerol
-
 ?
stearoyl-CoA + 1,2-dioleoyl-sn-glycerol fluorescent assay method using NBD-tagged diglycerol substrate Yarrowia lipolytica CoA + 1,2-dioleoyl-3-stearoyl-sn-glycerol
-
 ?
stearoyl-CoA + 1,2-dioleoyl-sn-glycerol fluorescent assay method using NBD-tagged diglycerol substrate Yarrowia lipolytica CLIB 122 CoA + 1,2-dioleoyl-3-stearoyl-sn-glycerol
-
 ?
stearoyl-CoA + 1,2-dioleoyl-sn-glycerol fluorescent assay method using NBD-tagged diglycerol substrate Yarrowia lipolytica E 150 CoA + 1,2-dioleoyl-3-stearoyl-sn-glycerol
-
 ?

Subunits

Subunits Comment Organism
? x * 99000, mutant Dga1pDELTA19, sequence calculation, x * 90000, mutant Dga1pDELTA85, sequence calculation Yarrowia lipolytica

Synonyms

Synonyms Comment Organism
acyl-CoA:diacylglycerol acyltransferase
-
 Yarrowia lipolytica
Dga1p
-
 Yarrowia lipolytica
DGAT
-
 Yarrowia lipolytica
DGAT2
-
 Yarrowia lipolytica
diacylglycerol acyltransferase 2
-
 Yarrowia lipolytica
YALI0E32769g
-
 Yarrowia lipolytica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
31
-
 assay at Yarrowia lipolytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at, non-fluorescent assay Yarrowia lipolytica
7.6
-
 assay at, fluorescent assay Yarrowia lipolytica

General Information

General Information Comment Organism
evolution the major triglycerol synthesizing enzyme in Yarrowia lipolytica is Dga1p which belongs to the DGAT2 family Yarrowia lipolytica
additional information the putative N-terminal transmembrane domain of Dga1p appears important, but not necessary for enzyme activity Yarrowia lipolytica