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Literature summary for 2.3.1.20 extracted from
- Multiple mechanisms contribute to increased neutral lipid accumulation in yeast producing recombinant variants of plant diacylglycerol acyltransferase 1 (2017), J. Biol. Chem., 292, 17819-17831 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain delta4 | Brassica napus |
| phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain delta4 | Camelina sativa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F449C | site-directed mutagenesis | Brassica napus |
| I144F | site-directed mutagenesis | Camelina sativa |
| I447F | site-directed mutagenesis | Brassica napus |
| I466F | site-directed mutagenesis | Camelina sativa |
| K289N | site-directed mutagenesis | Brassica napus |
| L441P | site-directed mutagenesis | Brassica napus |
| L460P | site-directed mutagenesis | Camelina sativa |
| L460P/I466F | site-directed mutagenesis | Camelina sativa |
| additional information | directed evolution of Brassica napus DGAT1 (BnaDGAT1) shows that one of the regions where amino acid residue substitutions lead to higher performance in BnaDGAT1 is in the ninth predicted transmembrane domain (PTMD9), generation of analogous mutant variants of Camelina sativa with amino acid residue substitutions in PTMD9, knock-in phenotypes, overview | Camelina sativa |
| additional information | directed evolution of Brassica napus DGAT1 (BnaDGAT1) shows that one of the regions where amino acid residue substitutions lead to higher performance in BnaDGAT1 is in the ninth predicted transmembrane domain (PTMD9), generation of several BnaDGAT1 variants with amino acid residue substitutions in PTMD9, knock-in phenotypes, overview | Brassica napus |
| V125F | site-directed mutagenesis | Brassica napus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| oleoyl-CoA | substrate inhibition | Brassica napus |  |
| oleoyl-CoA | substrate inhibition | Camelina sativa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Brassica napus | |
| additional information | - |
additional information | Michaelis-Menten kinetics | Camelina sativa | |
| 0.0005 | - |
oleoyl-CoA | pH 7.4, 30°C, recombinant mutant L460P/I466F | Camelina sativa | |
| 0.00071 | - |
oleoyl-CoA | pH 7.4, 30°C, recombinant mutant L460P | Camelina sativa | |
| 0.00076 | - |
oleoyl-CoA | pH 7.4, 30°C, recombinant wild-type enzyme | Camelina sativa | |
| 0.00099 | - |
oleoyl-CoA | pH 7.4, 30°C, recombinant mutant I466F | Camelina sativa | |
| 0.0013 | - |
oleoyl-CoA | pH 7.4, 30°C, recombinant wild-type enzyme | Brassica napus | |
| 0.00131 | - |
oleoyl-CoA | pH 7.4, 30°C, recombinant mutant L441P | Brassica napus | |
| 0.00173 | - |
oleoyl-CoA | pH 7.4, 30°C, recombinant mutant V125F | Brassica napus | |
| 0.00178 | - |
oleoyl-CoA | pH 7.4, 30°C, recombinant mutant I447F | Brassica napus | |
| 0.00223 | - |
oleoyl-CoA | pH 7.4, 30°C, recombinant mutant F449C | Brassica napus | |
| 0.00224 | - |
oleoyl-CoA | pH 7.4, 30°C, recombinant mutant I144F | Camelina sativa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum membrane | - |
Brassica napus | 5789 | - |
| endoplasmic reticulum membrane | - |
Camelina sativa | 5789 | - |
| microsome | - |
Brassica napus | - |
- |
| microsome | - |
Camelina sativa | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Brassica napus | |
| Mg2+ | required | Camelina sativa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + 1,2-diacyl-sn-glycerol | Brassica napus | - |
CoA + 1,2,3-triacylglycerol | - |
? | |
| acyl-CoA + 1,2-diacyl-sn-glycerol | Camelina sativa | - |
CoA + 1,2,3-triacylglycerol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica napus | Q9M4V2 | - |
- |
| Camelina sativa | A0A1S6PTV7 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + 1,2-diacyl-sn-glycerol | - |
Brassica napus | CoA + 1,2,3-triacylglycerol | - |
? | |
| acyl-CoA + 1,2-diacyl-sn-glycerol | - |
Camelina sativa | CoA + 1,2,3-triacylglycerol | - |
? | |
| oleoyl-CoA + 1,2-oleoyl-sn-glycerol | - |
Brassica napus | CoA + 1,2,3-trioleoylglycerol | - |
? | |
| oleoyl-CoA + 1,2-oleoyl-sn-glycerol | - |
Camelina sativa | CoA + 1,2,3-trioleoylglycerol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BnaDGAT1 | - |
Brassica napus |
| CsDGAT1B | - |
Camelina sativa |
| DGAT1 | - |
Brassica napus |
| DGAT1 | - |
Camelina sativa |
| DGAT1-4 | - |
Brassica napus |
| DGAT1.a | - |
Brassica napus |
| diacylglycerol acyltransferase 1 | - |
Brassica napus |
| diacylglycerol acyltransferase 1 | - |
Camelina sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Brassica napus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Brassica napus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00288 | - |
oleoyl-CoA | pH 7.4, 30°C, recombinant mutant I144F | Camelina sativa | |
| 0.00722 | - |
oleoyl-CoA | pH 7.4, 30°C, recombinant wild-type enzyme | Camelina sativa | |
| 0.00751 | - |
oleoyl-CoA | pH 7.4, 30°C, recombinant mutant I466F | Camelina sativa | |
| 0.01115 | - |
oleoyl-CoA | pH 7.4, 30°C, recombinant mutant V125F | Brassica napus | |
| 0.01161 | - |
oleoyl-CoA | pH 7.4, 30°C, recombinant mutant L460P/I466F | Camelina sativa | |
| 0.01342 | - |
oleoyl-CoA | pH 7.4, 30°C, recombinant wild-type enzyme | Brassica napus | |
| 0.015 | - |
oleoyl-CoA | pH 7.4, 30°C, recombinant mutant I447F | Brassica napus | |
| 0.01825 | - |
oleoyl-CoA | pH 7.4, 30°C, recombinant mutant F449C | Brassica napus | |
| 0.04864 | - |
oleoyl-CoA | pH 7.4, 30°C, recombinant mutant L460P | Camelina sativa | |
| 0.08937 | - |
oleoyl-CoA | pH 7.4, 30°C, recombinant mutant L441P | Brassica napus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | sequence alignment of DGAT1 reveals that the PTMD9 is conserved in many plant species | Brassica napus |
| additional information | sequence alignment of DGAT1 reveals that the PTMD9 is conserved in many plant species | Camelina sativa |
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Release 2023.1 (January 2023)
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