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Literature summary for 2.3.1.20 extracted from
- Characterization of type-2 diacylglycerol acyltransferases in the green microalga Chromochloris zofingiensis (2019), J. Agric. Food Chem., 67, 291-298 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 in microsomes | Chromochloris zofingiensis |
| recombinant expression of CzDGAT2 isozymes in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 in microsomes | Chromochloris zofingiensis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | when CzDGAT2s are individually expressed in a TAG-deficient Saccharomyces cerevisiae mutant strain H1246, only CzDGAT2C can restore the TAG biosynthesis and complement the strain | Chromochloris zofingiensis |
| additional information | when CzDGAT2s are individually expressed in a TAG-deficient Saccharomyces cerevisiae mutant strain, only CzDGAT2C can restore the TAG biosynthesis and complement the strain | Chromochloris zofingiensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Chromochloris zofingiensis | |
| 0.00307 | - |
oleoyl-CoA | isozyme CzDGAT2C, pH 7.4, 30°C | Chromochloris zofingiensis |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Chromochloris zofingiensis | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + 1,2-diacyl-sn-glycerol | Chromochloris zofingiensis | - |
CoA + 1,2,3-triacylglycerol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chromochloris zofingiensis | - |
- |
- |
| Chromochloris zofingiensis | A0A411PNH6 | - |
- |
| Chromochloris zofingiensis | A0A411PNL0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| preparation of TAG-deficient Saccharomyces cerevisiae mutant strain H1246 in microsomes with recombinant CzDGAT2 isozymes | Chromochloris zofingiensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + 1,2-diacyl-sn-glycerol | - |
Chromochloris zofingiensis | CoA + 1,2,3-triacylglycerol | - |
? | |
| additional information | isozyme CzDGAT2C displays typical DGAT activity | Chromochloris zofingiensis | ? | - |
- |
|
| additional information | isozyme CzDGAT2C displays typical DGAT activity, substrate specificity of isozyme CzDGAT2C, overview | Chromochloris zofingiensis | ? | - |
- |
|
| oleoyl-CoA + 1,2-diacyl-sn-glycerol | - |
Chromochloris zofingiensis | CoA + 1,2-diacyl-3-oleoyl-glycerol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | isozyme CzDGAT2C contains a short N-terminal hydrophilic tail (3 amino acid), followed by 2 adjacent transmembrane domains (positions 4-23 and 30-52) and a large C-terminal hydrophilic fragment | Chromochloris zofingiensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CzDGAT2 | - |
Chromochloris zofingiensis |
| CzDGAT2A | - |
Chromochloris zofingiensis |
| CzDGAT2B | - |
Chromochloris zofingiensis |
| CzDGAT2C | - |
Chromochloris zofingiensis |
| CzDGAT2D | - |
Chromochloris zofingiensis |
| CzDGAT2E | - |
Chromochloris zofingiensis |
| CzDGAT2F | - |
Chromochloris zofingiensis |
| CzDGAT2G | - |
Chromochloris zofingiensis |
| DGAT1A | - |
Chromochloris zofingiensis |
| DGAT1B | - |
Chromochloris zofingiensis |
| type-2 diacylglycerol acyltransferase | - |
Chromochloris zofingiensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Chromochloris zofingiensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Chromochloris zofingiensis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | comparison and analysis of functional motifs and evolutionary relationships of DGAT2s, protein-protein interaction analysis among CzDGAT2s, overview. N- and C-terminals are important for the enzyme activity of isozyme CzDGAT2C. Membrane yeast two-hybrid assay reveals a possible DGAT2 activity modulation via the formation of homodimer/heterodimer among different DGAT2 isoforms | Chromochloris zofingiensis |
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Release 2023.1 (January 2023)
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