Application | Comment | Organism |
---|---|---|
biotechnology | evaluation of the possibility of using the enzyme in biotechnological approaches where a reduction of polyunsaturated fatty acids in the oil is desired | Macadamia tetraphylla |
Cloned (Comment) | Organism |
---|---|
gene DGAT1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, semiquantitative RT-PCR enzym expression analysis, functional recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 and complementation, lipid analysis, overview | Macadamia tetraphylla |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum membrane | - |
Macadamia tetraphylla | 5789 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1,2-diacyl-sn-glycerol | Macadamia tetraphylla | - |
CoA + 1,2,3-triacylglycerol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Macadamia tetraphylla | A0A0U3E0G8 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Macadamia tetraphylla | - |
additional information | tissue expression analysis by semiquantitative RT-PCR, ubiquitous expression is observed among different vegetative organs of the plant, although it seems to be higher in developing seeds and particularly in germinating seed cotyledons | Macadamia tetraphylla | - |
seed | developing | Macadamia tetraphylla | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1,2-diacyl-sn-glycerol | - |
Macadamia tetraphylla | CoA + 1,2,3-triacylglycerol | - |
? | |
additional information | the enzyme MtDGAT1 has a substrate preference for monounsaturated over polyunsaturated fatty acids | Macadamia tetraphylla | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme protein structure is composed of a strongly hydrophilic N-terminal region of about 120 amino acids in MtDGAT1 followed by a long hydrophobic stretch (ca. 400 residues) with a predicted arrangement of 9-10 transmembrane helices that are likely to anchor the protein to the endoplasmic reticulum (ER) membrane | Macadamia tetraphylla |
Synonyms | Comment | Organism |
---|---|---|
MtDGAT1 | - |
Macadamia tetraphylla |
type 1 acyl-CoA:diacylglycerol acyltransferase | - |
Macadamia tetraphylla |
type I diacylglycerol acyltransferase | - |
Macadamia tetraphylla |
General Information | Comment | Organism |
---|---|---|
evolution | enzyme MtDGAT1 belongs to the DGAT1 family | Macadamia tetraphylla |
additional information | relevant is the presence of the acyl-CoA binding signature R122-G141 close to residues R156-N161 that have been involved in the active site and a DAG/phorbol ester binding motif. Two amino acid changes, G155 and T152, are found in MtDGAT1 within the putative acyl-CoA binding domain, replacing the highly conserved Ala and Ser residues that might affect acyl specificity of the enzyme. A previously reported leucine zipper motif is also found | Macadamia tetraphylla |
physiological function | type I diacylglycerol acyltransferase (MtDGAT1) is involved in triacylglycerol (TAG) biosynthesis and may contribute to biochemical mechanisms determining the particular fatty acid composition of Macadamia oil. Major contribution of DGAT enzymes to TAG biosynthesis in seed plants | Macadamia tetraphylla |