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Literature summary for 2.3.1.20 extracted from

  • Arroyo-Caro, J.A
    ; Manas-Fernandez, A.; Alonso, D.L.; Garcia-Maroto, F. Type I diacylglycerol acyltransferase (MtDGAT1) from Macadamia tetraphylla cloning, characterization, and impact of Its heterologous expression on triacylglycerol composition in yeast (2016), J. Agric. Food Chem., 64, 277-285 .
    View publication on PubMed

Application

Application Comment Organism
biotechnology evaluation of the possibility of using the enzyme in biotechnological approaches where a reduction of polyunsaturated fatty acids in the oil is desired Macadamia tetraphylla

Cloned(Commentary)

Cloned (Comment) Organism
gene DGAT1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, semiquantitative RT-PCR enzym expression analysis, functional recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 and complementation, lipid analysis, overview Macadamia tetraphylla

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum membrane
-
Macadamia tetraphylla 5789
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + 1,2-diacyl-sn-glycerol Macadamia tetraphylla
-
CoA + 1,2,3-triacylglycerol
-
?

Organism

Organism UniProt Comment Textmining
Macadamia tetraphylla A0A0U3E0G8
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
Macadamia tetraphylla
-
additional information tissue expression analysis by semiquantitative RT-PCR, ubiquitous expression is observed among different vegetative organs of the plant, although it seems to be higher in developing seeds and particularly in germinating seed cotyledons Macadamia tetraphylla
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seed developing Macadamia tetraphylla
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + 1,2-diacyl-sn-glycerol
-
Macadamia tetraphylla CoA + 1,2,3-triacylglycerol
-
?
additional information the enzyme MtDGAT1 has a substrate preference for monounsaturated over polyunsaturated fatty acids Macadamia tetraphylla ?
-
-

Subunits

Subunits Comment Organism
More the enzyme protein structure is composed of a strongly hydrophilic N-terminal region of about 120 amino acids in MtDGAT1 followed by a long hydrophobic stretch (ca. 400 residues) with a predicted arrangement of 9-10 transmembrane helices that are likely to anchor the protein to the endoplasmic reticulum (ER) membrane Macadamia tetraphylla

Synonyms

Synonyms Comment Organism
MtDGAT1
-
Macadamia tetraphylla
type 1 acyl-CoA:diacylglycerol acyltransferase
-
Macadamia tetraphylla
type I diacylglycerol acyltransferase
-
Macadamia tetraphylla

General Information

General Information Comment Organism
evolution enzyme MtDGAT1 belongs to the DGAT1 family Macadamia tetraphylla
additional information relevant is the presence of the acyl-CoA binding signature R122-G141 close to residues R156-N161 that have been involved in the active site and a DAG/phorbol ester binding motif. Two amino acid changes, G155 and T152, are found in MtDGAT1 within the putative acyl-CoA binding domain, replacing the highly conserved Ala and Ser residues that might affect acyl specificity of the enzyme. A previously reported leucine zipper motif is also found Macadamia tetraphylla
physiological function type I diacylglycerol acyltransferase (MtDGAT1) is involved in triacylglycerol (TAG) biosynthesis and may contribute to biochemical mechanisms determining the particular fatty acid composition of Macadamia oil. Major contribution of DGAT enzymes to TAG biosynthesis in seed plants Macadamia tetraphylla