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Literature summary for 2.3.1.20 extracted from
- The type 2 acyl-CoA diacylglycerol acyltransferase family of the oleaginous microalga Lobosphaera incisa (2018), BMC Plant Biol., 18, 298 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene DGAT1, single copy gene, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis of DGAT isozymes, recombinant expression in the TAG-deficient Saccharomyces cerevisiae strain H1246, LiDGATs of type 1 and 2 do not co-localize with each other at least in yeast | Lobosphaera incisa |
| gene DGAT2.1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis of DGAT isozymes, recombinant expression in the TAG-deficient Saccharomyces cerevisiae strain H1246, LiDGATs of type 1 and 2 do not co-localize with each other at least in yeast | Lobosphaera incisa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | the TAG-deficient Saccharomyces cerevisiae strain H1246 is complemented with LiDGAT1, LiDGAT2.1, LiDGAT2.2 and LiDGAT2.3 as well as with their double constructs followed by analysis of synthesized TAGs | Lobosphaera incisa |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | the DGAT2 isozymes all localize to distinct endoplasmic reticulum domains | Lobosphaera incisa | 5783 | - |
| endoplasmic reticulum | the DGAT2 isozymes all localize to distinct ER domains | Lobosphaera incisa | 5783 | - |
| membrane | integral transmembrane enzyme | Lobosphaera incisa | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + 1,2-diacyl-sn-glycerol | Lobosphaera incisa | - |
CoA + triacylglycerol | - |
? |  |
| acyl-CoA + 1,2-diacyl-sn-glycerol | Lobosphaera incisa | - |
CoA + 1,2,3-triacylglycerol | - |
? | |
| acyl-CoA + 1,2-diacyl-sn-glycerol | Lobosphaera incisa SAG 2468 | - |
CoA + 1,2,3-triacylglycerol | - |
? | |
| acyl-CoA + 1,2-diacyl-sn-glycerol | Lobosphaera incisa SAG 2468 | - |
CoA + triacylglycerol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lobosphaera incisa | A0A2H4UKL3 | the oleaginous microalgae has been first isolated from a glacier in Japan and named Myrmecia incisa to be renamed later to Parietochloris incisa in 2005 | - |
| Lobosphaera incisa | A0A386RTI5 | the oleaginous microalgae has been first isolated from a glacier in Japan and named Myrmecia incisa to be renamed later to Parietochloris incisa in 2005 | - |
| Lobosphaera incisa | A0A386RU68 | the oleaginous microalgae has been first isolated from a glacier in Japan and named Myrmecia incisa to be renamed later to Parietochloris incisa in 2005 | - |
| Lobosphaera incisa | A0A3Q8PZB6 | the oleaginous microalgae has been first isolated from a glacier in Japan and named Myrmecia incisa to be renamed later to Parietochloris incisa in 2005 | - |
| Lobosphaera incisa SAG 2468 | A0A2H4UKL3 | the oleaginous microalgae has been first isolated from a glacier in Japan and named Myrmecia incisa to be renamed later to Parietochloris incisa in 2005 | - |
| Lobosphaera incisa SAG 2468 | A0A386RTI5 | the oleaginous microalgae has been first isolated from a glacier in Japan and named Myrmecia incisa to be renamed later to Parietochloris incisa in 2005 | - |
| Lobosphaera incisa SAG 2468 | A0A386RU68 | the oleaginous microalgae has been first isolated from a glacier in Japan and named Myrmecia incisa to be renamed later to Parietochloris incisa in 2005 | - |
| Lobosphaera incisa SAG 2468 | A0A3Q8PZB6 | the oleaginous microalgae has been first isolated from a glacier in Japan and named Myrmecia incisa to be renamed later to Parietochloris incisa in 2005 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + 1,2-diacyl-sn-glycerol | - |
Lobosphaera incisa | CoA + triacylglycerol | - |
? | |
| acyl-CoA + 1,2-diacyl-sn-glycerol | - |
Lobosphaera incisa SAG 2468 | CoA + triacylglycerol | - |
? | |
| acyl-CoA + 1,2-diacyl-sn-glycerol | - |
Lobosphaera incisa | CoA + 1,2,3-triacylglycerol | - |
? | |
| acyl-CoA + 1,2-diacyl-sn-glycerol | - |
Lobosphaera incisa SAG 2468 | CoA + 1,2,3-triacylglycerol | - |
? | |
| additional information | recombinant isozyme LiDGAT2.2 shows significant triacylglycerol (TAG) production in response to exogenous arachidonic acid | Lobosphaera incisa | ? | - |
- |
|
| additional information | recombinant isozyme LiDGAT2.2 shows significant triacylglycerol (TAG) production in response to exogenous arachidonic acid | Lobosphaera incisa SAG 2468 | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acyl-CoA:1,2-diacylglycerol O-acyltransferase | UniProt | Lobosphaera incisa |
| DGAT | - |
Lobosphaera incisa |
| DGAT1 | - |
Lobosphaera incisa |
| DGAT2.1 | - |
Lobosphaera incisa |
| DGAT2.2 | - |
Lobosphaera incisa |
| DGAT2.3 | - |
Lobosphaera incisa |
| DGAT2A | - |
Lobosphaera incisa |
| DGAT2b | - |
Lobosphaera incisa |
| type 1 acyl-CoA:diacylglycerol acyltransferase | - |
Lobosphaera incisa |
| type 2 acyl-CoA:diacylglycerol acyltransferase | - |
Lobosphaera incisa |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | genome analysis reveals the presence of five putative DGAT isoforms in Lobosphaera incisa, including one DGAT of type 1, three DGATs of type 2 (isozymes LiDGAT2.1, LiDGAT2.2, and LiDGAT2.3), and a single isoform of a type 3 DGAT. Five DGATs encoded by the Lobosphaera incisa genome cluster differently within the eukaryotic DGAT family | Lobosphaera incisa |
| evolution | genome analysis reveals the presence of five putative DGAT isoforms in Lobosphaera incisa, including one DGAT of type 1, three DGATs of type 2, i.e. isozymes LiDGAT2.1, LiDGAT2.2, and LiDGAT2.3, and a single isoform of a type 3 DGAT. Five DGATs encoded by the Lobosphaera incisa genome cluster differently within the eukaryotic DGAT family | Lobosphaera incisa |
| metabolism | DGAT is considered as rate-limiting enzyme of TAG synthesis and accumulation in animals, plants and microbes | Lobosphaera incisa |
| additional information | in silico analyses of Lobosphaera incisa transcriptome data reveal 3 isoforms of DGAT type 2 | Lobosphaera incisa |
| physiological function | analysis of functional and cellular nature of type 1 and type 2 DGATs from Lobosphaera incisa, with LiDGAT1 being a major contributor to the TAG pool. LiDGATs of type 2 might be in turn involved in the incorporation of unusual fatty acids into TAG and thus regulate the composition of TAG | Lobosphaera incisa |
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