Cloned (Comment) | Organism |
---|---|
gene WSD4, phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain Rosetta (DE3), recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 results in the accumulation of wax esters, but not any detectable triacylglycerols (TAGs), indicating a predominant wax synthase (WS) activity in yeast | Thraustochytrium roseum |
gene WSD5, phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain Rosetta (DE3), recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 results in the accumulation of wax esters, but not any detectable triacylglycerols (TAGs), indicating a predominant wax synthase (WS) activity in yeast | Thraustochytrium roseum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
oleoyl-CoA | substrate inhibition; substrate inhibition | Thraustochytrium roseum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of wax synthase activity, overview | Thraustochytrium roseum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1,2-diacyl-sn-glycerol | Thraustochytrium roseum | - |
CoA + 1,2,3-triacylglycerol | - |
? | |
acyl-CoA + 1,2-diacyl-sn-glycerol | Thraustochytrium roseum ATCC28210 | - |
CoA + 1,2,3-triacylglycerol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thraustochytrium roseum | A0A1Z2RWY4 | - |
- |
Thraustochytrium roseum | A0A3G1ICG7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography | Thraustochytrium roseum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1,2-diacyl-sn-glycerol | - |
Thraustochytrium roseum | CoA + 1,2,3-triacylglycerol | - |
? | |
acyl-CoA + 1,2-diacyl-sn-glycerol | - |
Thraustochytrium roseum ATCC28210 | CoA + 1,2,3-triacylglycerol | - |
? | |
additional information | the recombinant protein purified from Escherichia coli strain Rosetta (DE3) has substantial wax synthase (WS) and lower diacylglycerol acyltransferase (DGAT) activity. Acyl-CoA substrate specificity of wax synthase activity, overview | Thraustochytrium roseum | ? | - |
- |
|
additional information | the recombinant protein purified from Escherichia coli strain Rosetta (DE3) has substantial wax synthase (WS) and lower diacylglycerol acyltransferase (DGAT) activity. Acyl-CoA substrate specificity of wax synthase activity, overview | Thraustochytrium roseum ATCC28210 | ? | - |
- |
|
palmitoyl-CoA + 1,2-diacyl-sn-glycerol | - |
Thraustochytrium roseum | CoA + 1,2-diacyl-3-palmitoylglycerol | - |
? | |
palmitoyl-CoA + 1,2-diacyl-sn-glycerol | - |
Thraustochytrium roseum ATCC28210 | CoA + 1,2-diacyl-3-palmitoylglycerol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
bifunctional WS/DGAT | - |
Thraustochytrium roseum |
More | see also EC 2.3.1.75 | Thraustochytrium roseum |
TrWSD4 | - |
Thraustochytrium roseum |
TrWSD5 | - |
Thraustochytrium roseum |
wax ester synthase/acyl-CoA diacylglycerol acyltransferase | - |
Thraustochytrium roseum |
wax ester synthase/acyl-CoA:diacylglycerol acyltransferase | - |
Thraustochytrium roseum |
WS/DGAT | - |
Thraustochytrium roseum |
WSD4 | - |
Thraustochytrium roseum |
WSD5 | - |
Thraustochytrium roseum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Thraustochytrium roseum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Thraustochytrium roseum |
General Information | Comment | Organism |
---|---|---|
evolution | distinct evolutionary origin of Thraustochytrium roseum bifunctional wax ester synthase/acyl-CoA diacylglycerol acyltransferases (WS/DGATs) from that of plant and bacterial WS/DGATs. Both TrWSD4 and TrWSD5 contain the conserved acyltransferase active-site motif (HHXXXDG), whose first histidine residue is substituted to serine and aspartate, respectively | Thraustochytrium roseum |
physiological function | two bifunctional enzymes, TrWSD4 and TrWSD5, from the marine protist Thraustochytrium roseum show wax ester synthase/acyl-CoA:diacylglycerol acyltransferase activity catalyzing wax ester and triacylglycerol synthesis. The WS/DGAT shows both in vitro wax synthase (WS) and DGAT activity, it is characterized as unspecific acyltransferase accepting a broad range of acyl-CoAs and fatty alcohols as substrates for WS activity but displaying substrate preference for medium-chain acyl-CoAs. In vivo characterization shows that the WS/DGAT predominantly functions as wax synthase | Thraustochytrium roseum |