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Literature summary for 2.3.1.20 extracted from

  • Zhang, N.; Mao, Z.; Luo, L.; Wan, X.; Huang, F.; Gong, Y.
    Two bifunctional enzymes from the marine protist Thraustochytrium roseum biochemical characterization of wax ester synthase/acyl-CoA diacylglycerol acyltransferase activity catalyzing wax ester and triacylglycerol synthesis (2017), Biotechnol. Biofuels, 10, 185 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene WSD4, phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain Rosetta (DE3), recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 results in the accumulation of wax esters, but not any detectable triacylglycerols (TAGs), indicating a predominant wax synthase (WS) activity in yeast Thraustochytrium roseum
gene WSD5, phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain Rosetta (DE3), recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 results in the accumulation of wax esters, but not any detectable triacylglycerols (TAGs), indicating a predominant wax synthase (WS) activity in yeast Thraustochytrium roseum

Inhibitors

Inhibitors Comment Organism Structure
oleoyl-CoA substrate inhibition; substrate inhibition Thraustochytrium roseum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics of wax synthase activity, overview Thraustochytrium roseum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + 1,2-diacyl-sn-glycerol Thraustochytrium roseum
-
CoA + 1,2,3-triacylglycerol
-
?
acyl-CoA + 1,2-diacyl-sn-glycerol Thraustochytrium roseum ATCC28210
-
CoA + 1,2,3-triacylglycerol
-
?

Organism

Organism UniProt Comment Textmining
Thraustochytrium roseum A0A1Z2RWY4
-
-
Thraustochytrium roseum A0A3G1ICG7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography Thraustochytrium roseum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + 1,2-diacyl-sn-glycerol
-
Thraustochytrium roseum CoA + 1,2,3-triacylglycerol
-
?
acyl-CoA + 1,2-diacyl-sn-glycerol
-
Thraustochytrium roseum ATCC28210 CoA + 1,2,3-triacylglycerol
-
?
additional information the recombinant protein purified from Escherichia coli strain Rosetta (DE3) has substantial wax synthase (WS) and lower diacylglycerol acyltransferase (DGAT) activity. Acyl-CoA substrate specificity of wax synthase activity, overview Thraustochytrium roseum ?
-
-
additional information the recombinant protein purified from Escherichia coli strain Rosetta (DE3) has substantial wax synthase (WS) and lower diacylglycerol acyltransferase (DGAT) activity. Acyl-CoA substrate specificity of wax synthase activity, overview Thraustochytrium roseum ATCC28210 ?
-
-
palmitoyl-CoA + 1,2-diacyl-sn-glycerol
-
Thraustochytrium roseum CoA + 1,2-diacyl-3-palmitoylglycerol
-
?
palmitoyl-CoA + 1,2-diacyl-sn-glycerol
-
Thraustochytrium roseum ATCC28210 CoA + 1,2-diacyl-3-palmitoylglycerol
-
?

Synonyms

Synonyms Comment Organism
bifunctional WS/DGAT
-
Thraustochytrium roseum
More see also EC 2.3.1.75 Thraustochytrium roseum
TrWSD4
-
Thraustochytrium roseum
TrWSD5
-
Thraustochytrium roseum
wax ester synthase/acyl-CoA diacylglycerol acyltransferase
-
Thraustochytrium roseum
wax ester synthase/acyl-CoA:diacylglycerol acyltransferase
-
Thraustochytrium roseum
WS/DGAT
-
Thraustochytrium roseum
WSD4
-
Thraustochytrium roseum
WSD5
-
Thraustochytrium roseum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Thraustochytrium roseum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Thraustochytrium roseum

General Information

General Information Comment Organism
evolution distinct evolutionary origin of Thraustochytrium roseum bifunctional wax ester synthase/acyl-CoA diacylglycerol acyltransferases (WS/DGATs) from that of plant and bacterial WS/DGATs. Both TrWSD4 and TrWSD5 contain the conserved acyltransferase active-site motif (HHXXXDG), whose first histidine residue is substituted to serine and aspartate, respectively Thraustochytrium roseum
physiological function two bifunctional enzymes, TrWSD4 and TrWSD5, from the marine protist Thraustochytrium roseum show wax ester synthase/acyl-CoA:diacylglycerol acyltransferase activity catalyzing wax ester and triacylglycerol synthesis. The WS/DGAT shows both in vitro wax synthase (WS) and DGAT activity, it is characterized as unspecific acyltransferase accepting a broad range of acyl-CoAs and fatty alcohols as substrates for WS activity but displaying substrate preference for medium-chain acyl-CoAs. In vivo characterization shows that the WS/DGAT predominantly functions as wax synthase Thraustochytrium roseum