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Literature summary for 2.3.1.20 extracted from

  • Lee, S.W.; Rho, M.C.; Park, H.R.; Choi, J.H.; Kang, J.Y.; Lee, J.W.; Kim, K.; Lee, H.S.; Kim, Y.K.
    Inhibition of diacylglycerol acyltransferase by alkamides isolated from the fruits of Piper longum and Piper nigrum (2006), J. Agric. Food Chem., 54, 9759-9763.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
(2E)-1-[2,4-dihydroxy-6-methoxy-3-[(2R)-5-methyl-2-(1-methylethenyl)hex-4-en-1-yl]phenyl]-3-(2,4-dihydroxyphenyl)prop-2-en-1-one IC50: 0.0098 mM Rattus norvegicus
(2E,4Z,8E)-N-[9-(3,4-methylenedioxyphenyl)-2,4,8-nonatrienoyl]piperidine IC50: 0.0298 mM, isolated from the extracts of fruits of Piper longum and Piper nigrum Rattus norvegicus
dehydropipernonaline noncompetitive inhibition, IC50: 0.0212 mM, isolated from the extracts of fruits of Piper longum and Piper nigrum Rattus norvegicus
additional information inhibition mechanism, the inhibitory activity of Piper fruits is influenced by the presence of the piperidine group rather than by isobutyl group, overview Rattus norvegicus
oleoyl-CoA substrate inhibition Rattus norvegicus
pipernonaline IC50: 0.0372 mM, isolated from the extracts of fruits of Piper longum and Piper nigrum Rattus norvegicus
piperrolein B IC50: 0.0201 mM, isolated from the extracts of fruits of Piper longum and Piper nigrum Rattus norvegicus
retrofractamide C IC50: 0.9 mM, above, isolated from the extracts of fruits of Piper longum and Piper nigrum Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0046
-
oleoyl-CoA pH 8.0, 25°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
male sprague-dawley rats
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Synonyms

Synonyms Comment Organism
DGAT
-
Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information inhibition kinetics Rattus norvegicus
0.0054
-
oleoyl-CoA pH 8.0, 25°C Rattus norvegicus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0098
-
IC50: 0.0098 mM Rattus norvegicus (2E)-1-[2,4-dihydroxy-6-methoxy-3-[(2R)-5-methyl-2-(1-methylethenyl)hex-4-en-1-yl]phenyl]-3-(2,4-dihydroxyphenyl)prop-2-en-1-one
0.0201
-
IC50: 0.0201 mM, isolated from the extracts of fruits of Piper longum and Piper nigrum Rattus norvegicus piperrolein B
0.0212
-
noncompetitive inhibition, IC50: 0.0212 mM, isolated from the extracts of fruits of Piper longum and Piper nigrum Rattus norvegicus dehydropipernonaline
0.0298
-
IC50: 0.0298 mM, isolated from the extracts of fruits of Piper longum and Piper nigrum Rattus norvegicus (2E,4Z,8E)-N-[9-(3,4-methylenedioxyphenyl)-2,4,8-nonatrienoyl]piperidine
0.0372
-
IC50: 0.0372 mM, isolated from the extracts of fruits of Piper longum and Piper nigrum Rattus norvegicus pipernonaline
0.9
-
IC50: 0.9 mM, above, isolated from the extracts of fruits of Piper longum and Piper nigrum Rattus norvegicus retrofractamide C