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Literature summary for 2.3.1.20 extracted from

  • Weselake, R.J.; Madhavji, M.; Szarka, S.J.; Patterson, N.A.; Wiehler, W.B.; Nykiforuk, C.L.; Burton, T.L.; Boora, P.S.; Mosimann, S.C.; Foroud, N.A.; Thibault, B.J.; Moloney, M.M.; Laroche, A.; Furukawa-Stoffer, T.L.
    Acyl-CoA-binding and self-associating properties of a recombinant 13.3 kDa N-terminal fragment of diacylglycerol acyltransferase-1 from oilseed rape (2006), BMC Biochem., 7, 24.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.20

Cloned(Commentary)

Cloned (Comment) Organism
expression of the poly-His-tagged N-terminal fragment of the enzyme, i.e. BnDGAT1(1–116)His6, in Escherichia coli strain BL21(DE3), BnDGAT1(1–116)His6 fails to react with protein in microsomal vesicles following treatment with proteinase K, and thus might be localized to the cytosolic side of the ER Brassica napus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information acyl-CoA substrate binding kinetics of the recombinant N-terminal fragment, overview Brassica napus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Brassica napus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13278
-
 x * 13278, recombinant poly-His-tagged N-terminal enzyme fragment comprising residues 1-116, sequence calculation Brassica napus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-1,2-diacylglycerol + acyl-CoA Brassica napus
-
 triacylglycerol + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Brassica napus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant poly-His-tagged N-terminal fragment of the enzyme, i.e. BnDGAT1(1–116)His6, from Escherichia coli strain BL21(DE3) Brassica napus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-diacyl-sn-glycerol + erucoyl-CoA
-
 Brassica napus 1,2-diacyl-3-erucoyl-sn-glycerol + CoA
-
 ?
1,2-diacyl-sn-glycerol + oleoyl-CoA
-
 Brassica napus 1,2-diacyl-3-oleoyl-sn-glycerol + CoA
-
 ?
sn-1,2-diacylglycerol + acyl-CoA
-
 Brassica napus triacylglycerol + CoA
-
 ?
sn-1,2-diacylglycerol + erucoyl-CoA i.e. a 22:1cisDELTA13-CoA Brassica napus triacylglycerol + CoA
-
 ?

Subunits

Subunits Comment Organism
? x * 13278, recombinant poly-His-tagged N-terminal enzyme fragment comprising residues 1-116, sequence calculation Brassica napus
More analysis of self-associating properties of BnDGAT1(1–116)His6 in an gel filtration approach, the recombinant N-terminal fragment associates to form tetramers Brassica napus

Synonyms

Synonyms Comment Organism
DGAT1
-
 Brassica napus
diacylglycerol acyltransferase-1
-
 Brassica napus