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Literature summary for 2.3.1.20 extracted from

  • Akao, T.; Kusaka, T.
    Solubilization of diglyceride acyltransferase from the membrane of Mycobacterium smegmatis (1976), J. Biochem., 80, 723-728.
    View publication on PubMed

General Stability

General Stability Organism
antichaotropic salts stabilize solubilized enzyme Mycolicibacterium smegmatis
asolectin, GSH, DTT do not stabilize Mycolicibacterium smegmatis
phosphate buffer, high concentration, stabilizes Mycolicibacterium smegmatis

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithio-bis(2-nitrobenzoic acid) i.e. DTNB, complete inhibition, DTT protects Mycolicibacterium smegmatis
cholate
-
Mycolicibacterium smegmatis
deoxycholate
-
Mycolicibacterium smegmatis
additional information diethyl-p-nitrophenylphosphate, diisopropyl fluorophosphate Mycolicibacterium smegmatis
N-ethylmaleimide inhibits solubilized enzyme, but not membrane-bound enzyme Mycolicibacterium smegmatis
p-chloromercuribenzene sulfonate complete inhibition, DTT protects Mycolicibacterium smegmatis
p-chloromercuribenzoate complete inhibition, DTT protects Mycolicibacterium smegmatis
sodiumdodecyl sulfate
-
Mycolicibacterium smegmatis
Triton WR1339
-
Mycolicibacterium smegmatis
Triton X-100
-
Mycolicibacterium smegmatis
Tween 20
-
Mycolicibacterium smegmatis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
palmitoyl-CoA
-
Mycolicibacterium smegmatis
0.014
-
1,2-diolein
-
Mycolicibacterium smegmatis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane firmly bound Mycolicibacterium smegmatis 16020
-

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone aqueous, stable to Mycolicibacterium smegmatis

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
solubilized by aqueous acetone Mycolicibacterium smegmatis

Storage Stability

Storage Stability Organism
0°C, inactivation after 4 days in Tris-HCl buffer Mycolicibacterium smegmatis
0°C, more than 50% of activity retained after 4 days in 0.25 M phosphate buffer Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-diolein + palmitoyl-CoA
-
Mycolicibacterium smegmatis 1,2-dioleoyl-3-palmitoyl-sn-glycerol + CoA
-
?
acyl-CoA + 1,2-diacylglycerol
-
Mycolicibacterium smegmatis CoA + triacylglycerol
-
?
palmitoyl-CoA + 1,2-diacylglycerol
-
Mycolicibacterium smegmatis CoA + 1,2-diacyl-3-palmitoylglycerol
-
?
palmitoyl-CoA + 1,2-dioleoyl-sn-glycerol
-
Mycolicibacterium smegmatis CoA + 1,2-dioleoyl-3-palmitoylglycerol
-
?
palmitoyl-CoA + 1,2-dipalmitin
-
Mycolicibacterium smegmatis CoA + tripalmitin
-
?
palmitoyl-CoA + 1,2-distearin
-
Mycolicibacterium smegmatis CoA + 3-palmitoyl-1,2-distearoylglycerol
-
?
palmitoyl-CoA + 1,3-diolein
-
Mycolicibacterium smegmatis CoA + 1,3-dioleoyl-2-palmitoylglycerol
-
?
palmitoyl-CoA + 1,3-dipalmitin
-
Mycolicibacterium smegmatis CoA + tripalmitin
-
?
palmitoyl-CoA + 1-monoolein
-
Mycolicibacterium smegmatis CoA + ?
-
?
palmitoyl-CoA + 1-monopalmitin
-
Mycolicibacterium smegmatis CoA + ?
-
?
palmitoyl-CoA + 1-monostearin
-
Mycolicibacterium smegmatis CoA + ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
Mycolicibacterium smegmatis