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Literature summary for 2.3.1.20 extracted from
- A highly sensitive assay for microsomal diacyglycerol acyltransferase activity from isolated fat cells (1976), Anal. Biochem., 71, 436-443.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| bovine serum albumin | 65% activation at 1 mg/ml, microsomal preparation | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Rattus norvegicus |  |
| ethanol | 43% inhibition when 0.04 ml are added to reaction mixture | Rattus norvegicus | |
| Tween 20 | 99% inhibition at 5 mg/ml, 89% inhibition at 1 mg/ml, 49% inhibition at 0.5 mg/ml | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Rattus norvegicus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 2.4fold at 8 mM | Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + 1,2-diacylglycerol | Rattus norvegicus | last reaction in triacylglycerol synthesis | CoA + triacylglycerol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
female Charles River CD | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adipocyte | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.05 | - |
- |
Rattus norvegicus |
| 0.05 | - |
above | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + 1,2-diacylglycerol | - |
Rattus norvegicus | CoA + triacylglycerol | - |
? | |
| acyl-CoA + 1,2-diacylglycerol | last reaction in triacylglycerol synthesis | Rattus norvegicus | CoA + triacylglycerol | - |
? | |
| palmitoyl-CoA + 1,2-diacylglycerol | diacylglycerol diluted in ethanol | Rattus norvegicus | CoA + 1,2-diacyl-3-palmitoylglycerol | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
assay at various temperatures from 24°C to 40°C | Rattus norvegicus |
| 25 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
microsomal preparation | Rattus norvegicus |
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Release 2023.1 (January 2023)
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