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Literature summary for 2.3.1.195 extracted from

  • D'Auria, J.C.; Pichersky, E.; Schaub, A.; Hansel, A.; Gershenzon, J.
    Characterization of a BAHD acyltransferase responsible for producing the green leaf volatile (Z)-3-hexen-1-yl acetate in Arabidopsis thaliana (2007), Plant J., 49, 194-207.
    View publication on PubMed
Search for more literature for 2.3.1.195

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, the coding region of At3g03480 is cloned into a vector that introduces an N-terminal 6His tag Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 5 mM, strong inhibition Arabidopsis thaliana
Co2+ 5 mM, strong inhibition Arabidopsis thaliana
Cu2+ 5 mM, strong inhibition Arabidopsis thaliana
Mg2+ 5 mM, strong inhibition Arabidopsis thaliana
Zn2+ 5 mM, strong inhibition Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 acetyl-CoA pH 7.7, 25°C, native enzyme, partially purified Arabidopsis thaliana
0.0385
-
 acetyl-CoA pH 7.7, 25°C, recombinant His6-tagged enzyme Arabidopsis thaliana
0.126
-
 (3Z)-hex-3-en-ol pH 7.7, 25°C, native enzyme, partially purified Arabidopsis thaliana
0.174
-
 1-Octanol pH 7.7, 25°C, recombinant His6-tagged enzyme Arabidopsis thaliana
0.251
-
 (3Z)-hex-3-en-ol pH 7.7, 25°C, recombinant His6-tagged enzyme Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 native plant protein, gel filtration Arabidopsis thaliana
50400
-
 1 * 50400, native plant protein, calculated from sequence Arabidopsis thaliana
51000
-
 His-tagged protein, gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + (3Z)-hex-3-en-ol Arabidopsis thaliana the major volatile released upon mechanical wounding of the leaves is (Z)-3-hexen-1-yl acetate (3Z)-hex-3-en-1-yl acetate + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9SRQ2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf enzyme activity in the leaves following wounding increases over time, but this response lagges significantly behind (Z)-3-hexen-1-yl acetate release. A maximum specific activity is reached 12 h after the initial wounding event. CHAT enzyme activity declines by 35% over the course of the next 12 h Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + (3Z)-hex-3-en-ol the major volatile released upon mechanical wounding of the leaves is (Z)-3-hexen-1-yl acetate Arabidopsis thaliana (3Z)-hex-3-en-1-yl acetate + CoA
-
 ?
acetyl-CoA + (3Z)-hex-3-en-ol the enzyme has the ability to accept several medium-chain-length aliphatic and benzyl-derived alcohols, but has highest catalytic efficiency with (Z)-3-hexen-1-ol. The catalytic efficiency with 1-octanol is 0.2% of the catalytic efficiency with (Z)-3-hexen-1-ol Arabidopsis thaliana (3Z)-hex-3-en-1-yl acetate + CoA
-
 ?
acetyl-CoA + 1-octanol the enzyme has the ability to accept several medium-chain-length aliphatic and benzyl-derived alcohols, but has highest catalytic efficiency with (Z)-3-hexen-1-ol. The catalytic efficiency with 1-octanol is 0.2% of the catalytic efficiency with (Z)-3-hexen-1-ol Arabidopsis thaliana octanyl acetate + CoA
-
 ?
additional information no activity with malonyl CoA and benzoyl CoA Arabidopsis thaliana ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 50400, native plant protein, calculated from sequence Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
acetyl CoA:(Z)-3-hexen-1-ol acetyltransferase
-
 Arabidopsis thaliana
At3g03480
-
 Arabidopsis thaliana
ChAT
-
 Arabidopsis thaliana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 30 min, stable Arabidopsis thaliana
37
-
 30 min, 13% loss of activity Arabidopsis thaliana
50
-
 30 min, complete loss of activity Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.125
-
 1-Octanol pH 7.7, 25°C, recombinant His6-tagged enzyme Arabidopsis thaliana
11.3
-
 (3Z)-hex-3-en-ol pH 7.7, 25°C, recombinant His6-tagged enzyme Arabidopsis thaliana
23
-
 acetyl-CoA pH 7.7, 25°C, recombinant His6-tagged enzyme Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1 7.3
-
 Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
6.3 9 pH 6.3: 89% of maximal activity, pH 9.0: about 45% of maximal activity Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction RNAi transgenic plants exhibiting a severe reduction in At3g03480 transcript levels emitt no (Z)-3-hexen-1-yl acetate Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.072
-
 1-Octanol pH 7.7, 25°C, recombinant His6-tagged enzyme Arabidopsis thaliana
45
-
 (3Z)-hex-3-en-ol pH 7.7, 25°C, recombinant His6-tagged enzyme Arabidopsis thaliana
597
-
 acetyl-CoA pH 7.7, 25°C, recombinant His6-tagged enzyme Arabidopsis thaliana